Sequence Analysis of Rifampicin Resistance Determining Region (RRDR) of Mycobacterium tuberculosis

Mitesh Shrestha, Hemanta Kumari Chaudhary, Pradip Kumar Chaudhary, Bal Hari Poudel


Mycobacterium tuberculosis has become the cause for one of the most dreadful disease which the mankind has ever known i.e. Tuberculosis. The organism holds the ability to infect multiple organs at a time resulting in multiple symptomatic presentations in pathogenic condition while in non-pathogenic condition, it can lay dormant and remain asymptomatic. The research work presented here aimed at sequencing of Rifampicin Resistance Determining Region (RRDR) of the rpoB gene present in phenotypically multidrug resistant M. tuberculosis. The findings showed that the major point of mutations to be present within this region was at codon 516, 526, and 531. Early diagnosis of multidrug resistance in any pathogen has become a pre – requisite for proper treatment and efficient elimination of pathogenic organisms from the host with minimal toxicity. Similarly, understanding the mutation dynamics of target genes also help in novel drug design and discovery.


Mycobacterium tuberculosis, Rifampicin, rpoB, RRDR, Sequence

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Chadha VK (2009) Progress towards millennium development goals for TB control in seven Asian countries. Indian Journal of Tuberculosis 56 (8): 30 – 43.

Farer LS, Lowell AM, Meador MP (1979) Extrapulmonary tuberculosis in the United States. American Journal of Ep-idemiology 109 (2): 205 – 217. doi: 10.1093/oxfordjournals.aje.a112675.

Zaman K (2010) Tuberculosis: A global health problem. Journal of Health, Population and Nutrition 28 (2): 111 – 113. doi: 10.3329/jhpn.v28i2.4879.

Smith I (2003) Mycobacterium tuberculosis pathogenesis and molecular determinants of virulence. Clinical Microbiology Review 16 (3): 463 – 496. doi: 10.1128/CMR.16.3.463 -496.2003.

Heep M, Rieger U, Beck D, Lehn N (2000) Mutations in the beginning of the rpoB gene can induce resistance to rifamycins in both Helicobacter pylori and Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 44 (4): 1075 – 1077. doi: 10.1128/AAC.44.4.1075-1077.2000.

Ramaswamy S, Musser JM (1998) Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tubercle and Lung Disease 79 (1): 3 – 29. doi: 10.1054/tuld.1998.0002.

Telenti A, Imboden P, Marchesi F et al. (1993) Direct, automated detection of rifampin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysis. Antimicrobial Agents and Chemotherapy 37 (10): 2054 – 2058. doi: 10.1128/AAC.37. 10.2054.

Rattan A, Kalia A, Ahmad N (1998) Multidrug-resistant Mycobacterium tuberculosis: molecular perspectives. Emerging Infectious Diseases 4 (2): 195 – 209. doi: 10.3201/eid0402.980207.

Musser JM (1995) Antimicrobial agent resistance in Mycobacteria: Molecular genetic insights. Clinical Microbiolo-gy Reviews 8 (4): 496 – 514.

Fan XY, Hu ZY, Xu FH et al. (2003) Rapid detection of rpoB gene mutations in Rifampin-resistant Mycobacterium tuberculosis isolated in shanghai by using the amplication refractory mutation system. Journal of Clinical Microbiology 41 (3): 993 – 997. doi: 10.1128/JCM.41.3.993-997.2003.

Cavusoglu C, Hilmioglu S, Guneri S, Bilgic A (2002) Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. Journal of Clinical Microbiology 40 (12): 4435 – 4438. doi: 10.1128/JCM.40.12. 4435-4438.2002

Kim BJ, Lee KH, Park BH et al. (1997) Mutations in the rpoB gene of Mycobacterium tuberculosis that interfere with PCR–Single-Strand conformation polymorphism analysis for rifampin susceptibility testing. Journal of Clini-cal Microbiology 35 (2): 492 – 494. doi: 10.1128/JCM.39.7.2610-2617. 2001.


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