Potential of Wild Yeast from Banana to Control Colletotrichum musae Fungi Caused Anthracnose Disease and Its Short Antagonistic Mechanism Assay

Authors

Keywords:

Colletotricum musae, Pichia sp., Metchnikowia sp., antagonist mechanism

Abstract

Anthracnose caused by Colletotrichum musae is one of several important disease in banana fruit. An effective, cheap, and safe control method are necessary as a postharvest disease control alternative. This research aimed to obtain and identify wild yeast from banana which was isolated from its peel that has antagonist ability effectively in controlling anthracnose disease of banana fruit.  This research was started with an isolation of C. musae and yeast from banana, followed by in vivo assay with count percentage antagonist level, slide culture, and in vivo assay to know pathogen incubation stage and incident disease level. The yeast that isolated from ambon variety was Candida sp. and Pichia sp. Metchnikowia sp. from kepok variety respectively.

Author Biography

Anton Muhibuddin, Brawijaya University

Plant and Disease Department, Faculty of Agriculture, University of Brawijaya

References

Ara I, Razwana H, Al-Othman M R, Bakir M A (2012) Studies of actinomycetes for biological control of Colletotrichum musae pathogen during post harvest antracnose of banana. African Journal of Microbiology Research 6 (17): 3879-3886.

Barnett J, Barnett L (2011) Yeast research: a historical approach. ASM Press. Washington.

Bergman LW (2001) Growth and maintenance of yeast. p: 9-32. in MacDonald PN (ed.) Method in molecular biology volume 177 two hybrid systems: methods and protocols. Humana Press Inc. New Jersey.

Dhiani H P (2012) Kemampuan antagonisme khamir filum Ascomycota dari tanaman saeh (Broussonetia papyrifera Vent.) asal Trowulan terhadap Aspergilus spp. UICC. Skripsi. Universitas Indonesia. Depok.

Droby S (2011) Postharvest pathology of tropical fruit and strategies for decay control p: 194-216. in Yahia EM (ed.). Postharvest biology and technology of tropical and subtropical fruits volume 1: fundamental isues. Woodhead Publishing. Cambridge.

Fenina S (2012) Kemampuan antagonisme khamir filum Basidiomycota dari tanaman saeh (Broussonetia papyfera Vent.) asal Trowulan terhadap Aspergilus spp. UICC. Skripsi. Universitas Indonesia. Depok.

Fitriati Y, Wiyono S, Sumaraw IO (2013) Khamir antagonis untuk pengendalian penyakit antraknosa pada buah avokad selama penyimpanan. Jurnal Fitopatologi Indonesia 9 (5): 153-159.

Jumiyati S, Bintari H, Mubarok I (2012) Isolasi dan identifikasi khamir secara morfologi di tanah kebun wisata pendidikan Universitas Negeri Semarang. Jurnal Biosaintifika 4 (1): 27-35.

Kardos L, Juhasz A, Palko GY, Olah J, Barkacs K, Zaray GY (2011) Comparing of mesophilic and thermophilic anaerobic fermentated sewage sludge based on chemical and biochemical test. Journal of Applied Ecology and Environmental Research 9 (3): 293-302

Kora, C, McDonald M R, Boland GJ (2008) New progress in the integrated management of sclerotinia rot of carrot p: 243-270. In Aiancio AK, Mukerji G (eds.) Integrated management of diseases caused by fungi, phytoplasma and bacteria. Springer Science. New York.

Li Z, Vizeacoumar FJ, Bahr S, Li J, Warringer J, Vizeacoumar FS et al. Systematic exploration of essential yeast gene function with temperature-sensitive mutants. Nature Biotechnology. 2011 Apr; 29(4):361-367

Martoredjo T (2013) Ilmu penyakit pasca panen. PT Bumi Aksara. Jakarta.

Muhibuddin A (2009) Kajian hubungan populasi Glomus fasciculatum dengan faktor lingkungan. AGRIVITA, Journal of Agricultural Science. 30 (1): 56-63.

Nurhalimah S, Nurhatika S, Muhibuddin A, (2015) Eksplorasi Mikoriza Vesikular Arbuskular (MVA) Indigenous Pada Tanah Regosol Di Pamekasan, Madura. Jurnal Sains dan Seni ITS. 3 (1): 30-34.

Prabawati S, Suyanti D, Setyabudi A (2008) Teknologi pasca panen dan teknik pengolahan buah pisang. Balai Besar Litbang Pasca Panen Pertanian. Jakarta Selatan.

Puspitasari AE, Abadi AL, Sulistyowati L (2014) Potensi khamir sebagai agens pengendali hayati patogen Colletotrichum sp. pada buah cabai, buncis, dan stroberi. Jurnal HPT 2 (3): 92-101.

Rumahlewang W, Amanupunyo HRD (2012) Patogenesitas Colletotrichum musae penyebab penyakit antraknosa pada beberapa varietas buah pisang. Jurnal Agrologia 1 (1): 76-81.

Sadat A, Tamrin S, Cicih (2015) Pengaruh pemeraman menggunakan batu karbit (CaC2) terhadap sifat fisik dan kimia buah pisang ambon kuning (Musa paradisiaca var. sapientum (L.) Kunt). Jurnal Rekayasa Pangan dan Pertanian 3 (4): 417-423.

Schisler DA, Jenisiewicz WJ, Boekhout T, Kurtzman CP. Agriculturally important yeast: biological control of field and postharvest disease using yeast antagonists, and yeast phathogens of plants the yeast. p: 45-52. in Kurztman CP, Fell JW, Boekhout T (eds.) The yeast a taxonomic study volume 1 fifth edition. Elsevier. London.

Silva CFB, Michereff SJ (2013) Biology of Colletotrichum spp. and epodemiologi of anthracnose in tropical fruit trees. Jornal of Revista Caatinga 26 (4): 130-138.

Spadaro D, Droby S (2015) Development of biocontrol products for postharvest diseases of fruit: the importance of elucidating the mechanism of action of yeast antagonist. Journal of Trends in Foos Science and Technology. 47 (2016): 39-49.

Sugipriatini D (2009) Potensi penggunaan khamir dan kitosan untuk pengendalian busuk buah Lasiodiplodia theobromae (Pat.) Griffon & Maubl. (syn. Botryodiplodia theobromae Pat.) pada buah mangga selama penyimpanan Tesis. Institut Pertanian Bogor. Bogor.

Thangamani PR, Kuppusamy P, Peeran MF, Gandhi K, Raguchander T (2011) Morphological and psysiological characterization of Colletotrichum musae the causal organism of banana anthracnose. World Journal of Agricultural Sciences 7 (6): 743-754.

Yarrow D (1998) Method for the isolation, maintenence, and identification of yeast p: 77-100 in Kurtzman CP, Feel JP. The Yeast a Taxonomic Study. Elsevier. Amsterdam.

Downloads

Published

2018-11-09

Issue

Section

Articles