Anthropometry, Fatty Liver, Plasma Lipid, and Adipose Tissue on Rat Wistar Induced Low-Protein Diet
DOI:
https://doi.org/10.11594/jtls.07.01.08Keywords:
Protein nergy malnutrition (PEM), low protein diet, anthropometry, fatty liverAbstract
Protein energy malnutrition problem still exists in developing country. In fact, Indonesia has faced it as double burden of nutrition problems. This study aims to develop a standardized diet for developing protein energy malnutrition (PEM) on the rat to support nutrition research. Low-protein modified AIM-93M diet (LP) has been given to rats for 12 weeks intervention compared to normal protein diet (ND). Anthropometry, plasma lipid, fatty liver and the weight of adipose tissue have been analysed. The LP group showed a significantly lower body weight, body length and waist circumference (p < 0.05), a more fatty liver marker shown in LP compared to ND (p < 0.05). This study suggests that giving low protein modified AIN-M93 diet promotes protein energy malnutrition in the rat.References
Fouque D, Kalantar-Zadeh K, Kopple J et al (2008) A proposed nomenclature and diagnostic. Kidney International 73 (4): 391-398. doi: 10.1038/sj.ki.5002585.
Gurung G (2010) Social determinants of protein-energy malnutrition: need to attack the causes of the causes. Journal of Health, Population, and Nutrition 28 (3): 308-309.
Purwestri RC, Scherbaum S, Inayati DA et al (2013) Impact of daily versus weekly supply of locally produced ready-to-use food on growth of moderately wasted children on Nias island, Indonesia. ISRN Nutrition 2013 (2013): 1–10. doi: 10.5402/2013/412145
Burchi F, Fanzo F, Frison E (2011) The role of food and nutrition system approaches in tackling hidden hunger. International Journal of Environmental Research and Public Health 8 (2): 358-373. doi: 10.3390/ijerph8020358.
Reeves PG (1993) AIN-93 purified diets for laboratory rodents: final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. The Journal of Nutrition 123 (11): 1939-1951.
Riesanti D, Masiana C, Herawati (2012) Kadar HDL, kadar LDL dan gambaran histopatologi aorta pada hewan model tikus (Rattus novergicus) hiperkolsterolemia dengan terapi ekstrak air benalu mangga (Dendrophthoe pentandra). Undergraduate Final Assignment. Brawijaya University, Veterinary Department.
Kudo T, Tamagawa T, Kawashima M et al. (2007) Attenuating effect of clock mutation on tryglyceride contents in the ICR mouse liver under a high high-fat diet. Journal of Biological Rhythms 22: 312-323. doi: 10.1177/0748730407302625.
Perhimpunan Dokter Spesialis Patologi Indonesia(2008) Pedoman Penanganan Bahan Pemeriksaan untuk Histologi. Jakarta, PT Roche Indonesia.
Rolls B (2000) The role of energy density in the over-consumption of fat. American Society for Nutritional Sciences 130 (2S Suppl): 268S-271S.
Muhammad I (2011) Manajemen Pemeliharaan Ayam Petelur di CV. Sari Makmur Farm Kabupaten Sukoharjo. Surakarta, Universitas Sebelas Maret.
Malta A, de Oliveira JC, Ribeiro TA et al. (2014) Low-protein diet in adult male rats has long-term effects on metabolism. Journal of Endocrinology 221: 285-295. doi: 10.1530/JOE-13-0473.
de Oliveira JC, Lisboa PC, de Moura EG et al. (2013) Poor pubertal protein nutrition disturbs glucose-induced insulin secretion process in pancreatic islets and programs rats in adulthood to increase fat accumulation. Journal of Endocrinology 216 (2): 195-206. doi: 10.1530/JOE-12-0408.
Tangendjaja B (2008) Distiller`s Dried Grains With Solubles (DDGS) untuk pakan. Wartazoa: Buletin Ilmu Peternakan Indonesia 18: 137–148.
Ahmadian M, Duncan RE, Sul SH (2008) Triacylglycerol metabolism in adipose tissue. Future Lipidol 2 (2): 229–237. doi:10.2217/17460875.2.2.229.
MacKay H, Khazall R, Patterson ZR et al. (2013) Rats perinatally exposed to food restriction and high-fat diet show differences in adipose tissue gene expression under chronic caloric restriction. Adipocyte 2 (4): 237–245. doi: 10.4161/adip.24752.
Leon (1993) Disease of the liver volume 7. Philadelphia, B Lippincott Company.
Kneeman JM, Misdraji J, Corey KE (2012) Secondary causes of nonalcoholic fatty liver disease. Therapeutic Advances in Gastroenterology 5 (3): 199–207. doi: 10.1177/1756283X11430859.
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