Improvement of Trimethylamine Uptake by Euphorbia milii: Effect of Inoculated Bacteria


  • Dian Siswanto Brawijaya University King Mongkut's University of Technology Thonburi
  • Paitip Thiravetyan King Mongkut's University of Technology Thonburi



Trimethylamine, Euphorbia milii, indole-3-acetic acid, bacteria, absorption


In the last few years, a great emphasis has been placed on phytoremediation of indoor air pollution studies. However, limited work has been addressed to observe the bacteria potential to assist the phytoremediation process of trimethylamine (TMA). In this work, the ability of 4 different bacteria for individual TMA removal and IAA production were observed. In addition, the enhancement of TMA removal efficiency by Euphorbia milii with various inoculating bacteria were investigated. Bacillus thuringiensis, Citrobacter amalonaticus Y19, Bacillus nealsonii, and white colony-soil bacteria (WCSB) were able to absorb TMA and produce IAA individually. B. thuringiensis and C. amalonaticus Y19 were the two most effective bacteria to improve TMA removal efficiency by the plant. Since concentrations of IAA production by individual bacterium were highly correlated with TMA removal efficiency by plants in early periods of fumigation and highly correlated with leaf IAA production of bacterially inoculated plants, two predicted mechanisms on improving TMA uptake by bacterially inoculated plants are presented: (1) bacteria migration from plant roots to leaves increases leaf IAA concentration and (2) increasing concentration of bacterially inoculated root IAA inhibits transportation of IAA from leaves to roots, resulting in higher leaf IAA concentration. The higher concentration of leaf IAA is suggested to be a factor to increase stomatal opening which improves TMA removal efficiency of the plant.


Khaksar G, Treesubsuntorn C, Thiravetyan P (2016) Endophytic Bacillus cereus ERBP – Clitoria ternatea interactions: Potentials for enhancement of gaseous formaldehyde removal. Environ Exp Bot 126: 10-20.

Boraphech P, Thiravetyan P (2015) Removal of trimethylamine (fishy odor) by C3 and CAM plants. Environ Sci Pollut R 22: 11543-11557.

Liffourrena A, Lucchesi G (2014) Degradation of trimethylamine by immobilized cells of Pseudomonas putida A (ATCC 12633). Int Biodeter Biodegr 90: 88-92.

Ma Y, Prasad MNV, Rajkumar M et al. (2011) Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnol Adv 29: 248-258.

Hardoim PR, Overbeek LS van, Elsas JD van (2008) Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol 16(10): 463-471.

Kim SG, Bae HS, Lee ST (2001) A novel denitrifying bacterial isolate that degrades trimethylamine both aerobically and anaerobically via two different pathways. Arch Microbiol 176: 271-277.

Kim SG, Bae HS, Oh HM et al. (2003) Isolation and characterization of novel halotolerant and/or halophilic denitrifying bacteria with versatile metabolic pathways for the degradation of trimethylamine FEMS Microbiol Lett 225: 263-269.

Ho KL, Chung YC, Lin YH et al. (2008) Biofiltration of trimethylamine, dimethylamine, and methylamine by immobilized Paracoccus sp. CP2 and Arthrobacter sp. CP1. Chemosphere 72: 250-256.

Rappert S, Müller R (2005) Microbial degradation of selected odorous substances. Waste Manage 25: 887-907.

Etesami H, Alikhani HA, Hosseini HM (2015) Indole-3-acetic acid (IAA) production trait, a useful screening to select endophytic and rhizosphere competent bacteria for rice growth promoting agents. MethodsX 2: 72-78.

Sriprapat W, Thiravetyan P (2016) Efficacy of ornamental plants for benzene removal from contaminated air and water: Effect of plant associated bacteria. Int Biodeter Biodegr. In Press.

Siswanto D, Chhon Y, and Triravetyan P (2016) Uptake and degradation of trimethylamine by Euphorbia milii. Environ Sci Pollut Res. In Press.

Gordon SA, Weber RP (1951) Colometric estimation of indolacetic acid. Plant Physiol 26: 192-195.

Tanuja, Bisht SC, Mishra PK (2013) Ascending migration of endophytic Bacillus thuringiensis and assessment of benefits to different legumes of N.W. Himalayas. Eur J Soil Biol 56: 56-64.

Shin MN, Shim J, You Y et al. (2012) Characterization of lead resistant endophytic Bacillus sp. MN3-4 and its potential for promoting lead accumulation in metal hyperaccumulator Alnus firma. J Hazard Matter 199-200:314-320.

Leveau JHJ, Lindow SE (2005) Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl Environ Microb 71 (5): 2365-2371.

Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbio Rev 31: 425-448.

Kawaguchi M, Syono K (1996) The excessive production of indole-3-acetic acid and its significance in studies of biosynthesis of this regulator of plant growth and development. Plant Cell Physiol 37(8): 1043-1048.

Kasan K (2013) Auxin and the integration of environmental signals into plant root development. Annals of Botany. 11 pages. doi:10.1093/aob/mct229.

Vidal-Quist JC, Rogers HJ, Mahenthiralingam E et al. (2013) Bacillus thuringiensis colonises plant roots in a phylogeny-dependent manner. FEMS Microbiol Eco 86: 474-489.

Bahgat MMM, Kawasthy SA, Bous MME et al. (2014) Characterization of endophytic bacteria isolated from medicinal plant Capparissinaica Veill. and analyze its bioactive flavonoid. Indian Journal of Applied Research 4 (11): 5-13.

Tan ZY, Peng GX, Xu PZ et al. (2009) Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryza rufipogon Griff. Chinese Sci Bull 54: 2839-2848.

Grunewald W, Noorden GV, Isterdael GV et al. (2009) Manipulation of auxin transport in plant roots during Rhizobium symbiosis and nematode parasitism. The Plant Cell 21: 2553-2562.

Willmer C, Fricker M (1996) Stomata. Second edition. Editors: M. Black and B. Charlwood. London, Springer-Science+Business Media.

Ramnath L, Tamara B, Govinden R (2014) Method optimization for denaturing gradient gel electrophoresis (DGGE) analysis of microflora from Eucalyptus sp. wood chips intended for pulping. African journal of biotechnology 13(3): 256-265.

Sansinenea E, Ortiz A (2013) An antibiotic from Bacillus thuringiensis against Gram-negative bacteria. Biochem & Pharmacol 2: 1-2.