Bioactivity of Sweet Flag (Acorus calamus Linnaeus) Essential Oils against Spodoptera litura Fabricius (Lepidoptera: Noctuidae)

Dewi Melani, Toto Himawan, Aminudin Afandhi

Abstract


The study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a com-pletely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and anti-feedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatog-raphy-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone. Keywords:  Acorus calamus, essential oils, mortality,  antifeedant,  Spodoptera litura

The study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a completely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and antifeedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatography-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone.

Keywords


Acorus calamus; antifeedant; essential oils; mortality; Spodoptera litura

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References


Singh SP, Jalali SK (1997) Management of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). In: Spodoptera litura in India. Proc. Natl. Sci. Forum Spodoptera litura, 2-4 Apr 1996.

Direktorat Perlindungan Tanaman Pangan (2012) Laporan Luas dan Serangan Hama Penyakit Tanaman Pangan di In-donesia. Direktorat Perlindungan Tanaman Pangan. Jakarta.

Jacobson M (1989) Botanical pesticides, past present and future. In: Insecticides of plant origin. (Ed. Arnason, J.T.). Proceeding of the American Chemical Society, Washington, D.C.: 1-10.

Adeyemi M, Hassan M (2010) The potential of secondary metabolites in plant material as deterents against insect pests: A review. Afr. J. Pure Appl. Chem. 4(11) : 243-246.

Rattan RS (2010) Mechanism of Action of Insecticidal Sec-ondary Metabolites of Plant Origin. Crop Protect., 29(9): 913-920.

Hasan MU, Sagheer M, Ullah E et al (2006) Insecticidal activity of different doses of Acorus calamus oil against Tro-goderma granarium (everts). J. Agriculture Science. 43 (1-2): 55-58.

Jiyavorranant T, Chanbang Y, Supyen D et al (2003) The Effect of Acorus calamus Linn ands Stemona tuberosa Lour. Extract on the Insect Pest, Pluttela xylostella (Linnaeus). Ac-ta Hort. 597: 223-229.

Koul O (1987) Antifeedant and growth inhibitory effect of calamus oil and neem oil on Spodoptera litura under labor-atory conditions. Phytoparasitica.15 (3): 169-180.

Koul O, Smirle MJ, Isman MB (1990) Asarones from Aco-rus calamus L. oil their effect on feeding behaviour and die-tary dtilization Peridroma saucia. J. Chem. Ecol. 16(6) : 1911-1920.

Tariq RM, Naqvi SNH, Chundhary MI et al (2010) Im-portance and implemetation of esential oil of Pakistanian Acorus calamus Linn, as a biopesticide. Pak. J.Bot. 42(3) : 2043-2050.

Senthilkumar A, Venkatesalu V (2012) Larvicidal potential of Acorus calamus L. essential oil against filarial vector mosquito Cx. quinquefasciatus (Diptera: Culicidae). Asian Pacific Journal of Tropical Disease. 2 (4) : 324–326.

Adfa M, Fio L, Neva PM et al (2015) Termiticidal activity of Acorus calamus Linn. rhizomes and its main constituents against Coptotermes curvignathus Holmgren. Journal of Asia-Pacific Entomology. 18 (1) : 47–50.

Sahayaraj K (1998) Antifeedant effect of some Plant extracts on the Asian armyworm, Spodoptera litura (Fabricius)”, Curent Science, 74(6): 532-526.

Bentley MD, Leonard DE, Stoddard WF et al (1984) Pyrrol-izidinalkaloids as larval feeding deterrents for spruce bud worm, Choristoneura fumiferana (Lepidoptera: Tortricidae) Annals of the Entomological Society of America, 77(4): 393-397.

Arivoli S, Samuel T (2012) Antifeedant activity of plant extracts against Spodoptera litura (Fab.) (Lepidoptera: Noc-tuidae). American-Eurasian J. Agric. & Environ. Sci., 12 (6): 764-768.

Abbot WS (1925) A method of computing the effectiveness of incesticide. J.Econ. Entomol. 18:265-267.

Finney DJ (1971) Probit analysis. London, Cambridge Uni-versity Press.

Boucias DG, Pendland JC (1998) Principles of insect pa-thology. London, Kluwer Academic Publisher.

Hung SY, Boucias DG (1996) Phenoloksidase activity in hemolymph of naïve and Beauveria bassiana-Infected Spodoptera exigua Larvae. Florida, Academic Press, Inc.

Oh MH, Houghton PJ, Whang WK et al (2004) Screening of Korean herbal medicines used to improve cognitive func-tion for anti-cholinesterase activity. Phytomedicine. 11 (6) : 544–548.

Matsumura F (1976) Toxicology of insecticides. New York, Plenum Press.

Sharma PR, Om PS, Bhaskar PS (2008) Effect of sweet flag rhizome oil (Acorus calamus) on hemogram and ultrastruc-ture of hemocytes of the tobacco armyworm, Spodoptera litura (Lepidoptera: Noctuidae). Micron. 39 (5): 544–551.

Isman M (2002) Insect antifeedants: In pesticide outlook. Royal Soc.Chem.: 152-157.

Meyer JR (2006) Chemoreceptors. NC State University. Available online at http://www.cals.ncsu.edu/, Accessed: 2016.

Lee HK, Chan P, Young-Joon A (2002) Insecticidal activi-ties of asarones identified in Acorus gramineus rhizome against Nilaparvata lugens (Homoptera: Elphacidae) and Plutella xylostella (Lepidoptera: Yponomeutoidae). Appl. Entomol. Zool., 37(3): 459-464.




DOI: http://dx.doi.org/10.11594/jtls.06.02.04

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