Bleach Solution Requirement for Hatching of Daphnia magna Resting Eggs
Keywords:Bleach solution, Daphnia magna resting eggs, hatching
AbstractDaphnia (water fleas) belong to the zooplankton group called Cladocerans have sexual reproduction when conditions less favorable that produce diapausing eggs are enclosed in the ephippium. Hatching ephippial eggs in the laboratory is important in ecological, toxicology, genetical, and evolutionary studies. This study aims to improve the current methods of egg hatching from ephippium. Each of 50 ephippium were treated together by placing them in a glass jar and adding 50 mL bleach solution (sodium hypochlorite). Concentrations of sodium hypochlorite used in this experiment were 0%, 0.5%, 1%, 2%, 4% and 8%. These concentration treatments were crossed with the following exposure times (1, 2, 4, 8, 16, and 32 minutes). Culturing was done in 80 mL of artificial Daphnia medium, incubated in constant light and temperatures 20Â°C for 25 days. There were two repetitions in this experiment that were run at the same time. Result of this experiment showed that pretreatment with 0.5-8% bleach solution significantly increases the yield of total hatch rate of Daphnia magna resting eggs by about 21% over unbleached control. However, there was no significant difference among the bleach treatments. Concentration of bleach solution 0.5%, 1% and 4% significantly accelerated the time period until the first hatching (first day hatching). Difference of exposure time (1 - 32 minutes) at each concentration treatments were not influence the yield of total hatch and the time period until first hatching.
Haag CR, Hottinger JW, Riek M, Ebert D (2002) Strong inbreeding depression in a Daphnia metapopulation. Evolution 56 (3): 518-526.
Curtis H, Barnes NS (1989) Biology: Fifth Edition. Worth Publishers Inc., New York. New York.
Terra NR, Feiden IR (2003) Reproduction and survival of Daphnia magna Straus, 1820 (Crustacea: Cladocera) under different hardness conditions. Acta Limnol. Bras. 15
Balcer MD, Korda NL, Dodson SI (1984) Zooplankton of the Great Lakes. University of Wisconsin Press. Madioson.
HobÃ¦k A, Larsson P (1990). Sex determination in Daphnia magna. Ecology 71 (6): 2255-2268.
Winsor GL, Innes DJ (2002) Sexual reproduction in Daphnia pulex (Crustacea: Cladocera): observations on male mating behaviour and avoidance of inbreading. Freshwater Biology 47: 441-450.
Haag CR, SakwiÃ±ska O, Ebert D (2003) Test of synergistic interaction between infection and inbreeding in Daphnia magna. Evolution 57 (4): 777-783.
Hebert PDN (1978)The population biology of Daphnia (Crustacea, Daphnidae). Biological Reviews 53: 387-426.
Innes DJ (1997) Sexual reproduction of Daphnia pulex in a temporary habitat. Oecologia 111: 53-60.
Hutchinson GE (1967) A treatise on limnology Vol II. John Wiley and Sons. New York.
Mellors WK (1975) Selective predation of ephippial Daphnia and the resistance of ephippial eggs to digestion. Ecology 56: 974-980.
Slusarczyk M (2001) Food threshold for diapause in Daphnia under the threat of fish predation. Ecology 82(4): 1089-1096.
Pancella JR, Stross RG (1963) Light induced hatching of Daphnia resting eggs. Chesapeake Sciences 4 (3): 135-140
Stross RG (1966) Light and temperature requirements for diapause development and release in Daphnia. Ecology 47: 368-374.
Stollewerk A (2010). The water flea Daphnia â€“ a â€˜newâ€™ model system for ecology and evolution?. Journal of Biology 9: 21.
Roulin AC, Routtu J, Hall MD et al. (2013) Local adaptation of sex induction in a facultative sexual crustacean: insight from QTL mapping and natural populations of Daphnia magna. Molecular Ecology 22: 3567-3579.
Davison J (1969) Activation of the ephippial egg of Daphnia pulex. The Journal of General Physiology 53: 562-575.
The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Journal of Tropical Life Science is licensed under Creative Commons Attribution-NonCommercial 4.0 International License