Evaluation of Pulasan (Nephelium ramboutan-ake) Genetic Diversity in Bogor, West Java, Using Microsatellite Markers

Amelia Luisyane Puhili, Tatik Chikmawati, Nina Ratna Djuita


Pulasan (Nephelium ramboutan-ake (Labill.) Leenh) fruit is highly similar to rambutan rapiah (Nephelium lappaceum) fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA)9(CA)2(GA)4 dan LMLY12 (CT)11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na), the number of effective alleles (Ne), Shannon information index (I), heterozygosity (He), and the percentage of polymorphism (PLP). The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313). The genetic diversity within a population (61%) was higher than that among populations (39%). A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA). The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.


Bogor, genetic diversity, Nephelium ramboutan-ake, microsatellite

Full Text:



[BK-Kehati] Balai Kliring Keanekaragaman Hayati Indonesia (2013) Tenggaring. [Internet]. [diunduh pada 24 November 2013]. Tersedia pada: http://www.bk.menlh.go.id/.

Lim TK (2013) Edible Medicinal and Non Medicinal Plants: Volume 6, Fruits. Canberra (AU): Springer Netherlands.

Boer D (2007) Keragaman dan Struktur Genetik Populasi Jati Sulawesi Tenggara Berdasarkan Marka Mikrosatelit. PHD thesis. Institut Pertanian Bogor.

Qian W, Ge S, Hong DH (2001) Genetic variation within and among populations of a wild rice (Oryza granulate) from China detected by RAPD and ISSR markers. Theor Appl Genet. 102: 440-449.

Brown SM, Hopkins MS, Mitchell SE et al (1996) Multiple methods for the identification of polymorphic simple sequence repeats (SSRs) in sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet. 93: 190r-198.

Gianfranceschi L, Seglias N, Tarchini R, Komjanc M, Gessler C (1998) Simple sequence repeats for the genetic analysis of apple. Theor Appl Genet. 96: 1069-1076.

Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus.12: 13-15.

Sim CC, Mahani MC, Choong YC, Salma I (2005) Transferability of SSR markers from lychee (Litchi chinensis Sonn.) to pulasan (Nephelium ramboutan-ake L.) Fruits. 60:379-384.

Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research. Bioinformatics 28: 2537-2539.

Rohlf FJ (2000) NTSYS pc Numerical Taxonomy and Multivariate Analysis System Version 2.1. New York (US): Applied Biostatistic Inc.

Li WH, Graur D (1991) Fundamentals of Molecular Evolution. Massachusetts (US): Sinaur Associates Inc.

Hamrick JL, Godt MJW, and Sherman-Broyles SL (1992) Factors influencing levels of genetic diversity in woody plantspecies. New For. 6: 95–124.

Ferguson A (1980) Biochemical Systematics and Evolution. London (GB): The Queens University of Belfast.

Nei M (1987) Molecular Evolutionary Genetics. New York (US): Columbia Press.

Pai AC (1992) Dasar-Dasar Genetika. Jakarta (ID): Penerbit Erlangga.

Hartati D, Rimbawanto A, Taryono et al (2007) Pendugaan keragaman genetik di dalam dan antar provenan pulai (Alstonia scholaris (L) R. Br.) menggunakan penanda RAPD. Jurnal Pemuliaan Tanaman Hutan 2: 98-98.

DOI: http://dx.doi.org/10.11594/jtls.06.03.09

Copyright (c) 2016 Journal of Tropical Life Science