Effects of Artemisin and Moringa oleifera Extract Combination on CD4+ and CD8+ Percentage of Mice Infected with Plasmodium berghei
Abstract
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This research aims to examine the effect of Artemisin and Moringa oleifera leaf extract combination on the percentage of CD4+ and CD8+ T cell of mice infected with P.berghei. CD4+ and CD8+ T cells have important role in eliminating Plasmodium intracellular parasite that causes malaria infection. Artemisin is a potent antimalarial that kills the parasite through free radicals production. Excessive free radicals damage the immune cells, including CD4+ and CD8+ T cells. Flavonoid (quercetin and kaempferol) bioactive on Moringa leaves is a powerful antioxidant and anti-inflammatory, and is expected to prevent and decrease the adverse effects of Artemisin. This experimental post-test group research was conducted on six groups, i.e. normal mice (negative control), P.berghei infected mice without treatment (positive control), and four other groups, i.e. P.berghei infected mice and treated with Artemisin 0.004mg/gBW (A), and combinations of Artemisin 0.004mg/gBW and Moringa leaf extract 0.125mg/gBW (DK1), 0.250mg/gBW (DK2), and 0.500mg/gBW (DK3). On day 3 and 7, blood samples from each group were drawn randomly, parasitemia degree was calculated microscopically (magnification 1000 times), the percentage of CD4+ and CD8+ T cells was determined using flowcytometry. The results of this study indicated that the administration of Artemisin and Moringa leaf extract combination for 7 days significant increased the percentage of CD4 + T cells in DK2 (p=0.001) and DK3 (p=0.000), and decreased the degree of parasitemia in DK1 (p=0.000), DK2 (p=0.000), and DK3 (p=0.000), however CD8 + T cells show no difference. There was a relationship between Artemisin and Moringa leaf extract combination with the degree of parasitemia (p=0.000) and the percentage of CD4+ T cells (p = 0.000), but not on CD8+ T cells.
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Keywords: parasitemia, CD4+ and CD8+ T cells, Moringa oleifera
References
WHO. World Malaria Report 2011.
Erdman, L. K. Host Inflammatory Pathways in Malaria Infection: Potential Therapeutic Targets and Biomarkers of Disease Severity. Doctor of Philosophy, University of Toronto; 2011: p. 236-306.
Kassa, D., Petros, B., Mesele, T., Hailu, E. & Wolday, D. Characterization of Peripheral Blood Lymphocyte Subsets in Patients with Acute Plasmodium falciparum and P. vivax Malaria Infections at Wonji Sugar Estate, Ethiopia. Clinic and Vaccine Immunology. 2006; 13: 376-379.
Dogruman-Al, F., Fildan, I., Kustimur, S., Ceber, K. & Imir, T. Determination of the expression of lymphocyte surface markers and cytokine levels in a mouse model of Plasmodium berghei. New Microbiologica. 2009; 32: 285-291.
Roihatul M. Aktivitas Antimalaria ekstrak Batang Talikuning (Anamirta cocculus) dan Kombinasinya dengan Artemisin pada Mencit yang diinfeksi Plasmodium berghei. Pascasarjana, Universitas Brawijaya Malang, 2010: p. 20-44.
Hadzic, T. Malaria and Reactive Oxgygen Species: The Dance of Death. Departement of Pathology. Iowa City: The University of Ipwa. 2005: 1-8.
Fahey, J. W. Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1. Trees for Life Journal. 2005: 2120-2185.
Coppin, J. A Study of The Nutritional and Medicinal Values of Moringa oleifera Leaves from Sub-Saharan Africa : Ghana, Rwanda, Senegal and Zambia. Master of Science, The State University of New Jersey. 2008: 1-101.
Kasolo, J. N., Bomenya, G. S., Ojok, L., Ochieng, J. & Ogwal-Okeng, J. W. Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. Journal of Medicinal Plants Research. 2010; 4 (9): 753-755.
Goyal, B. R., Agrawal, B. G., Goyal, R. K. & Metha, A. A. Phyto-pharmacology of Moringa oleifera lam. Natural Product Radiance. 2007; 6: 347-353.
Sudha, Asdaq, S. M. B., Sunil, Dhamingi & Chandrakala, G. K. Immunomodulatory Activity Of Methanolic Leaf Extract Of Moringa Oleifera Animals. Indian J Physiol Pharmacol. 2007: 54: 133-140.
Siddhuraju, P. & Becker, K. Antioxidant properties of various extracts of total phenolic constituens from three different agriclimatic origins of drumstick tree (Moringa oleifera lam.) leaves. J. Agric. Food Chem. 2003; 51: 2144-2155.
Schmidt. Induction of pro-inflammatory mediators in Plasmodium berghei infected BALB/c mice breaks blood-brain-barrier and leads to cerebral malaria in an IL-12 dependent manner. Microbes and Infection. 2011; 13 (10): 828-36.
White, N. J. & Breman, J. G. Malaria and Babeosis: Diseases Caused by Red Blood Cell Parasites. In: Kasper, D. L., Fauci, A. S., Dan L. Longo, M., Braunwald, E., Hauser, S. L. & Jameson, J. L. (eds.) Harrison's Principal of Internal Medicine. 16th ed. New York, Chicago: McGraw-Hill; 2008: p. 1219-1231.
Patel, JP., Gami, B., and Patel, K. Evaluation of in vitro schizonticidal properties of acetone extract of some Indian medicinal plants. Advances in Biological Research. 2010; 4 (5): 253-258.
Frita, R., Carapau, D., Mota, M. M. & Hanscheid, T. In Vivo Hemozoin Kinetics after Clearance of Plasmodium berghei Infection in Mice. Malaria Research and Treatment. 2012: 1-9.
Gaikwad, S. B., Mohan, D. G. K. & Reddy, K. J. Moringa oleirefa Leaves: Immunomodulation in Winstar Albino Rats. International Journal of Pharmaceutical Sciences. 2011; 3 (5): 426-430.
Jung, J.-H., Kang, J.-I. & Kim, H.-S. Effect of Quercetin on impaired function in mice exposed to irradiation. Nutrition Research and Practice. 2012; 6 (4): 301-307.
Gargouri, B., Mansour, R. B., Abdallah, F. B., Elfekih, A., Lassoued, S. & Khaled, H. Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral. 2011: 10-149.
Caldeon-Montano, J. M., Burgos-Moron, E., Perez-Guerrero, C. & Lopez-Lazaro, M. A Review on the Dietary Flavonoid Kaempferol. Medical Chemistry. 2011; 11 (4): 298-344.
Acroute D. V. A., Lafitte S., Dive D., Khalife J. & Pierrot C. A Role For CD4+ and CD8+ Cells and Not For CD25+ Cells In The Control Of Plasmodium Berghei Anka Blood Stage Parasites In Rats. Parasite Journal. 2010; 10: 1051-1053.
Banji, O. J. F, Banji, D, Kavitha R. Immunomodulatory effects of alcoholic and hydroalcoholic extracts of Moringa olifera Lam leaves. Indian Journal of Experimental Biology. 2012; 50: 270-276.
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