DEGRADATION OF PETROLEUM REFINERY WASTE BY A CONSORTIUM OF HYDROCARBONOCLASTIC BACTERIA ON SEVERAL C:N:P RATIO

Abstract

Bioremediation is an alternative method to treat petroleum waste using microorganism into nontoxic end
product. The method is relatively cheap, effective, and environmental friendly. A key factor influencing
bioremediation process for petroleum refinery waste treatment is C:N:P ratio of bacterial growth medium. The
objective of this research was to obtain C:N:P ratio of Stone Mineral Salt Solution (SMSS) medium that allow
optimal degradation of petroleum refinery waste by consortium of hydrocarbonoclastic bacteria. C:N:P ratio of
SMSS medium was adjusted to ratio of 100:10:1, 100:10:0.5, 100:5:1, and 100:5:0.5. We demonstrate that optimal
degradation of petroleum refinery waste by a consortium of hydrocarbonoclastic bacteria was achieved in SMSS
medium with C:N:P ratio of 100:5:1. It allowed 66.55% degradation of total petroleum hydrocarbon (TPH) and
85.18 % decrease of chemical oxygen demand (COD) value.