Genome-wide investigation and characterization of heat shock transcription factors in papaya (Carica papaya): Mining of the HSF transcription factor in papaya

Thi Man Le; Sahar Azar; Huyen Thi Thanh Tran; Ha Duc Chu; Quyen Thi Xuan Vu; Lan Thi Mai Tran; Hong Viet La; Chi Toan Le; Phi Bang Cao

doi:10.11594/jtls.14.03.02

Authors

Thi Man Le Faculty of Natural Sciences, Hung Vuong University, Phu Tho Province 35000, Vietnam https://orcid.org/0009-0008-9839-6117
Sahar Azar Faculty of Sciences, Fanar Campus Lebanese University, Baabda, 1003, Lebanon https://orcid.org/0000-0003-1760-7901
Huyen Thi Thanh Tran Faculty of Biology, Hanoi National University of Education, Hanoi City 122300, Vietnam https://orcid.org/0000-0002-5351-6401
Ha Duc Chu Faculty of Agricultural Technology, University of Engineering and Technology, Vietnam National University Hanoi, Xuan Thuy Road, Cau Giay District, Hanoi City 122300, Vietnam https://orcid.org/0000-0003-0087-9994
Quyen Thi Xuan Vu Faculty of Natural Sciences, Hung Vuong University, Phu Tho Province 35000, Vietnam https://orcid.org/0009-0006-2967-3315
Lan Thi Mai Tran Faculty of Natural Sciences, Hung Vuong University, Phu Tho Province 35000, Vietnam https://orcid.org/0009-0004-5222-7431
Hong Viet La Institute of Research and Application, Hanoi Pedagogical University 2, Phuc Yen City, Vinh Phuc Province 280000, Vietnam https://orcid.org/0000-0002-6016-5857
Chi Toan Le Faculty of Biology and Agricultural Engineering, Hanoi Pedagogical University 2, Vinh Phuc Province 280000, Vietnam https://orcid.org/0000-0002-5339-4440
Phi Bang Cao Faculty of Natural Sciences, Hung Vuong University https://orcid.org/0000-0001-9374-3839

DOI:

https://doi.org/10.11594/jtls.14.03.02

Keywords:

Carica papaya, characteristic, gene expression, genome-wide identification, heat shock transcription factor

Abstract

Heat shock transcription factors (Hsf-s) have been considered one of the major regulatory proteins that play important roles in various biological processes during the growth and development of plants. Unfortunately, no comprehensive studies of the Hsf family in papaya (Carica papaya), a popular tropical fruit crop, have been performed, even the papaya genome assembly has been released recently. In this study, a total of 18 members of the Hsf family, namely CpHsf-s have been identified in papaya. Based on various computational tools, a systematic characterization of the CpHsf family, such as gene structure, physic-chemical features, categorization, and protein-protein interaction, has been conducted. We found that the physic-chemical properties of the CpHsf proteins were highly variable, while all proteins were hydrophilic and localized in the nucleus. Our classification indicated that the CpHsf proteins could be categorized into three groups, including HsfA, HsfB, and HsfC, and members in the same clade share similar gene structure. According to the previous RNA-Seq datasets, our re-analysis revealed that the CpHsf genes exhibited different expressions in various major organs or tissues during fruit ripening and under the ultra-low temperature stress. Taken together, our study could provide a list of candidate genes for further functional characterization towards the improvement of stress tolerance in papaya.

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Genome-wide investigation and characterization of heat shock transcription factors in papaya (Carica papaya)

Mining of the HSF transcription factor in papaya

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