Effect of Nigella Sativa Extract on Inflammatory Cells, Interleukin-10, Interferon-γ and Histological of Kidney in Monosodium Glutamate-Induced Rats

Abdalrauf A Mahmud Yousif, Hidayat Sujuti, Edi Widjajanto


There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), a flavor enhancer was unhealthy. Herbal medicine Nigella sativa (NS) has antioxidant properties able to cure the toxic induced by MSG. This study aimed to evaluate the risks of excessive use of MSG and to study the role of NS to inhibit inflammation and renal damage. Treated rats (twenty four male wistar rats) were divided into six group and analyzed by measuring the cells in blood, interleukin-10, interferon-γ serum levels by ELISA method and remove kidneys for histological examination. Histological of kidney for all groups except control, were showed different abnormalities include congestion of some blood vessels, hemorrhage  between tubules, widening in the renal tubules, revealed severe dilatation of Bowman's capsule and shrinkage of glomeruli, and areas of huge vacuole, were observed compared with control. Interleukin-10 was reduced in Groups 2,3,4 and 5, whereas increase in NS group compared with control. Interferon-γ was increased in groups 2,3,4 and reduced in groups 5,6 compared with control.  Eosinophil was increased in groups 2,5 and reduced in groups 3,4, 6 compared with control. This present study showed that administration of MSG to rats induced many changes effects on inflammatory cells, cytokines and histological of kidneys. NS has benefit in blood parameters, whereas harmful on kidney at these doses.

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Husarova V, and Ostatnikova D. Monosodium Glutamate Toxic Effects and Their Implications For Human Intake: A Review. IBIMA Publishing JMED Research 2013; DOI: 10.5171/2013.608765.

Ilegbedion I, Onyije F, Chibuike O. Infiltration of Inflammatory Cells in the Ovary Following Oral Administration of Monosodium Glutamate. Bangladesh Journal of Medical Science 2013; 12:413-418

Savcheniuk O, Virchenko O, Falalyeyeva T, Beregova T, Babenko L, Lazarenko L, Demchenko O, Bubnov R, Spivak M. The Efficacy of Probiotics for Monosodium Glutamate-Induced Obesity: Dietology Concerns and Opportunities for Prevention. The EPMA Journal 2014; 5:2

Hassan Z, Arafa M, Soliman W, Atteia H, Al-Saeed H. The Effects of Monosodium Glutamate on Thymic and Splenic Immune Functions and Role of Recovery (Biochemical and Histological Study). J Cytol Histol 2014; 5:6.

He Ka L, Daviglus M, Dyer A, Horn L, Garside D, Zhu L, Guo D, Wu Y, Zhou B., And Stamler J. Association of Monosodium Glutamate Intake with Overweight in Chinese Adults: The Intermap Study. Obesity 2008; 16(8): 1875–1880

Abass M. And Abd El-Haleem M. Evaluation of Monosodium Glutamate Induced Neurotoxicity and Nephrotoxicity in Adult Male Albino Rats. Journal of American Science 2011; 7(8): 264-276

Shivasharan, Nagakannan P, Thippeswamy B, Veerapur V. Protective Effect of Calendula Officinalis L. Flowers Against Monosodium Glutamate Induced Oxidative Stress and Excitotoxic Brain Damage in Rats. Ind J Clin Biochem 2013; 28(3): 292–298

Euteum P, Kim DK, Kim S, Sapkota K, Kim S, Kim CS, Chun HS. Protective Effects of N-Acetylcysteine Against Monosodium Glutamate-Induced Astrocytic Cell Death, Food And Chemical Toxicology 2014; 67:1–9

Xu L, Sun J, Lu R, Ji Q, Xu J. Effect of Glutamate on Inflammatory Responses of Intestine and Brain after Focal Cerebral Ischemia. World Journal of Gastroenterology 2005; 11(5): 733-736

Yaqub H, Abdel Baky N., Attia H. And Faddah L. Hepatoprotective Effect of N-Acetyl Cysteine and/or β-Carotene on Monosodium Glutamate-Induced Toxicity in Rats. Research Journal of Medicine and Medical Sciences 2008; 3(2): 206-215

Shimada A, Cairns B, Vad N, Ulriksen K, Pedersen A, Svensson P, Baad-Hansen L. Headache and Mechanical Sensitization of Human Pericranial Muscles After Repeated Intake of Monosodium Glutamate (MSG). The Journal of Headache and Pain 2013; 14:2

Pavlovic V, and Sarac M. The Role of Ascorbic Acid and Monosodium Glutamate in Thymocyte Apoptosis. Bratisl Lek Listy 2010; 111(6): 357-360

Sharma A, Wongkham C, Prasongwattanav, Boonnate P, Thanan R, Reungjui S, Cha’on U. Proteomic Analysis of Kidney in Rats Chronically Exposed to Monosodium 2014.

Afeefy A, Mahmoud M, Arafa M. Effect of Honey on Monosodium Glutamate Induced Nephrotoxicity (Histological and Electron Microscopic Studies). Journal of American Science 2012; 8(1s): 146-156

Paarakh P, and Linn NS. A Comprehensive Review. Indian Journal of Natural Products and Resources 2010; 1(4): 409-429

Sandhu KS, and Rana AC. A Review of Plant Nigella Sativa: A Brief Consideration of Its Pharmacognostic Characters. Chemical Constituents and Therapeutic Benefits. An International Journal of Pharmaceutical Sciences 2013; 4

Salem M. Immunomodulatory and Therapeutic Properties of the Nigella Sativa L. Seed. International Immunopharmacology 2005; 5 : 1749–1770

Gholamnezhad Z, Boskabady M and Hosseini M. Effect of Nigella Sativa on Immune Response in Treadmill Exercised Rat. BMC Complementary and Alternative Medicine 2014;14:437

Dollah M, Parhizkar S, Abdul Latiff L, Bin Hassan M. Toxicity Effect of Nigella Sativa on The Liver Function of Rats. Advanced Pharmaceutical Bulletin 2013; 3(1): 97-102

Hadi V, Alizadeh M, Hosseini H. Effects of Nigella Sativa Oil Extract on Inflammatory Cytokine Response and Oxidative Stress Status in Patients With Rheumatoid Arthritis: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial. Avicenna J Phytomed 2014.

Panahi M, Namjoyan F, Shakerin Z. Evaluation of Antioxidant Effects of Nigella Sativa Extract on The Ultra Structure of Neural Tube Defects in Diabetic Rats's Offspring. Jundishapur Journal of Natural Pharmaceutical Products 2011; 6(1): 16-23

Fahmy H, Noor N, Faten F, Elsayed A, Radwan N. Nigella Sativa as An Anti-Inflammatory and Promising Remyelinating Agent in The Cortex and Hippocampus of Experimental Autoimmune Encephalomyelitis-Induced Rats. The Journal of Basic & Applied Zoology 2014; 67:182–195

Ibrahim Z, Ishizuka M, Soliman M, Elbohi K, Sobhy W, Muzandu K, Elkattawy A, Sakamoto K., Fujita S. Protection by Nigella Sativa Against Carbon Tetrachloride-Induced Downregulation of Hepatic Cytochrome P450 Isozymes in Rats. Japanese Journal of Veterinary Research 2008; 56(3): 119-12

Liua X, Jong-Hyouk P, Abd El-Aty A, Assayed M, Minoru S, Jae-Han S. Isolation of Volatiles From Nigella Sativa Seeds Using Microwave-Assisted Extraction: Effect of Whole Extracts on Canine and Murine CYP1A, Biomed. Chromatogr 2013; 27: 938–945

Dixit S, Rani P, Anand A, Khatri K, Chauhan R, Bharihoke V. To Study The Effect of Monosodium Glutamate on Histomorphometry of Cortex of Kidney in Adult Albino Rats. Renfail 2014; 36(2): 266–270

Ortiz G, Bitzer-Quintero O, Beas Zarate C, Rodriguez-Reynoso S, Larios- Arceo F, Velazquez- Brizuela I, Pacheco-Moises F, Rosales-Corral S. Monosodium Glutamate-Induced Damage in Liver And Kidney: A Morphological and Biochemical Approach, Biomedicine & Pharmacotherapy 2006; 60: 86-91

Moussa E, Al Mulhim J. Modulating Effect of Nigella Sativa on Renal Structural Changes by Monosodium Glutamate in Female Mice, Egypt. Acad. J. Biolog. Sci 2013; 5(2): 33-45

Inuwa H, Aina V, Gabi B, Aim Ola I, Ja’afaru L. Determination of Nephrotoxicity and Hepatoxicity of Monosodium Glutamate (MSG) Consumption. British Journal of Pharmacology And Toxicology 2011; 2(3): 148-153

Attia H, Faddah L, Yaqub H. Trans-Retinol Precursor and/or N-Acetyl Cysteine Protects Against Monosodium Glutamte-Induced Nephrotoxicity in Rats. Journal of Applied Sciences Research 2008; 4(12): 2108-2119.

Onaolapo A, Onaolapo O, Mosaku T, Akanji O, Abiodun OA. Histological Study of the Hepatic and Renal Effects of Subchronic Low Dose Oral Monosodium Glutamate in Swiss Albino Mice. British Journal Of Medicine & Medical Research 2013; 3(2): 294-306

Zaghlol D, Kamel E, Mohammed D. And Abbas N. The Possible Toxic Effect of Different Doses of Nigella Sativa Oil on the Histological Structure of the Liver And Renal Cortex of Adult Male Albino Rats. The Egyptian Journal of Histology 2012; 35:127-136

Yildiz F, Coban S, Terzi A, Savas M, Bitiren M, Celik H, Aksoy N. Protective effects of Nigella sativa against ischemia-reperfusion injury of Kidneys, Renal Failure 2010; 32(1):126-31. 414-3141170

Alarcon-Aguilar F, Almanza-Perez J, Blancas G, Angeles S, Garcia-Macedo R, Roman R, Cruz M. Glycine Regulates The Production of Pro-Inflammatory Cytokines in Lean And Monosodium Glutamate-Obese Mice. European Journal of Pharmacology 2008; 599: 152–158

Chaparro-Huerta V, Flores-Soto M, Gudino-Cabrera G, Rivera-Cervantes M, Bitzer-Quintero O, Beas-Zarate C. Role of P38 MAPK and Pro-Inflammatory Cytokines Expression in Glutamate-Induced Neuronal Death of Neonatal Rats. Int. J. Devl Neuroscience 2008; 26: 487–495

Roman-Ramos R, Almanza-Perez J, Garcia-Macedo R, Blancas-Floresg, Fortis Barrera A, Jasso E, Garcia-Lorenzana M, Campos-Sepulveda A, Cruz M, Alarcon-Aguilar F. Monosodium Glutamate Neonatal Intoxication Associated With Obesity in Adult Stage Is Characterized By Chronic Inflammation And Increased mRNA Expression of Peroxisome Proliferator-Activated Receptors in Mice. Basic & Clinical Pharmacology & Toxicology 2010; 108: 406–413

Hernández-Bautista R, Alarcón-Aguilar F, Escobar-Villanueva M, Almanza-Pérez J, Merino-Aguilar J, Fainstein M, López-Diazguerrero N. Biochemical Alterations During The Obese-Aging Process in Female and Male Monosodium Glutamate (MSG)-Treated Mice. Int. J. Mol. Sci. 2014;15: 11473-11494

Zhang J, and Jianxiong A. Cytokines, Inflammation and Pain. Int Anesthesiol Clin. 2009; 45(2): 27–37

Keyhanmanesh R, Pejman L, Omrani H, Mirzamohammadi Z, Shahbazfar A. The Effect of Single Dose of Thymoquinone, the Main Constituents of Nigella Sativa in Guinea Pig Model of Asthma. Bioimpacts 2014; 4(2): 75-81. Doi: 10.5681/Bi.2014.006

Behm C, and Ovington K. The Role of Eosinophils in Parasitic Helminth Infections: Insights from Genetically Modified Mice. Parasitology Today 2000; 16(5): 202-209

Stevenson D. Monosodium Glutamate and Asthma, American Society for Nutitional Sciences 2000; 10678-10738.

Nakanishi Y, Tsuneyama K, Fujimoto M, Salunga T, Nomoto K, Jun-Ling An, Takanoy., Iizuka S., Nagata M., Suzuki W., Shimada T., Aburada M., Nakano M., Selmi C., Gershwin M. Monosodium Glutamate (MSG): A Villain and Promoter of Liver Inflammation and Dysplasia. Journal of Autoimmunity 2008; 30: 42-50

Jovic Z, Veselinovic M, Vasic K, Stankovic-Djordjevic D, Cekic S, Milovanovic M, Sarac M. Monosodium Glutamate Induces Apoptosis in Naïve and Memory Human B Cells. Bratisl Lek Listy 2009; 110(10): 636-640.

Al-Sa'aidi J, Dawood K, and Latif A. Immunomodulatory Effect of Nigella Sativa Seed Extract in Male Rabbits Treated With Dexamethasone, Iraqi Journal of Veterinary Sciences 2012; 26

Kamil Z. Effect of Crude Oil of Black Seeds (Nigella Sativa) on White Blood Cell and Hematocrit of Male Albino Mice Treated With Low Toxic Dose of Paracetamol. Medical Journal of Babylon 2013; 10(4).

You Lu. Eosinophils, Their Progenitors and T Helper Cells in Allergic Airway Inflammation. Institute of Medicine at Sahlgrenska Academy. University of Gothenburg 2011.

Suzukawa M, Nagaseh, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M., and Ohta K. Leptin Enhances Survival and Induces Migration, Degranulation, and Cytokine Synthesis of Human Basophils. The Journal of Immunology 2011.

Siracusa M, Kim B, Spergel J, and Artis D. Basophils and Allergic Inflammation. J Allergy Clin Immunol 2013; 132:789-801.

Al-Zendi S, Jasim A, AL-Mousawi A. The Effect of Hot Water and Ethanol Extract of Nigella Sativa in Immune System of Albino Mice. Journal of College of Science For Salmawomen 2009; (2) 6

Falcone F, Haas H, and Gibbs B. The Human Basophil: A New Appreciation of Its Role in Immune Responses, The American Society of Hematology 2000; 96(13): 4028-4038

Naz H. Nigella Sativa: The Miraculous Herb. Pak. J. Biochem. Mol. Biol. 2011; 44(1): 44-48

Tayman C, Cekmez F, Kafa L, Canpolat F, Cetinkaya M, Tonbul A, Uysal S, Tunc T, Saricia S. Protective Effects of Nigella Sativa Oil in Hyperoxia-Induced Lung Injury. Arch Bronconeumol 2012.

Ashaolu J, Ukwenya V, Okonoboh A, Ghazal O, Jimoh A. Effect Of Monosodium Glutamate on Hematological Parameters in Wistar Rats. International Journal of Medicine and Medical Sciences 2011; 3(6): 219-222

Butterfield T, Best T, Merrick M. The Dual Roles of Neutrophils and Macrophages in Inflammation: A Critical Balance between Tissue Damage and Repair. Journal of Athletic Training 2006; 41(4): 457–465

Kolaczkowska E, and Kubes P. Neutrophil Recruitment and Function in Health and Inflammation, Nature Reviews Immunology 2013; 13: 159-175.

Hriscu M, Saulea G, Vidraşcu N, Baciu I. Effects of Monosodium Glutamate on Blood Neutrophils Phagocytic Activity and Phagocytic Responsein Mice. Rom J Physiol. 1997; 34(1-4): 95-101.

Gordon S, and Taylor P. Monocyte and Macrophage Heterogeneity. Nature Reviews Immunology 2005; 5: 953-964

Wrigley B, Lip G, and Shantsila E. The Role of Monocytes and Inflammation in The Pathophysiology of Heart Failure, European Journal of Heart Failure 2011; 13: 1161–1171

DOI: http://dx.doi.org/10.11594/jtls.06.01.04

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