Identification of Two Enzymes for Trehalose Synthesis and Their Potential Function in Growth and Development in Peanut (Arachis hypogaea): Genome Analysis of the Encoding Trehalose Synthesis Enzymes in Peanut

Ha Duc Chu; Yen Thi Hai Tran; Chau Thuy Pham; Thi Ngoc Quynh Le; Thi Thanh Huyen Tran; Trung Quoc Nguyen; Huy Gioi Dong; Van Tien Tran; Phi Bang Cao

doi:10.11594/jtls.14.01.10

Authors

Ha Duc Chu Faculty of Agricultural Technology, University of Engineering and Technology, Vietnam National University Hanoi, Xuan Thuy Road, Cau Giay District, Hanoi City 122300, Vietnam https://orcid.org/0000-0003-0087-9994
Tran Thi Hai Yen Faculty of Agricultural Technology, University of Engineering and Technology, Vietnam. Faculty of Biology, Hanoi National University of Education, Hanoi City 122300, Vietnam National University Hanoi, Xuan Thuy Road, Cau Giay District, Hanoi City 122300, Vietnam https://orcid.org/0009-0007-2895-311X
Chau Thuy Pham Faculty of Agricultural Technology, University of Engineering and Technology, Vietnam National University Hanoi, Xuan Thuy Road, Cau Giay District, Hanoi City 122300, Vietnam https://orcid.org/0000-0001-9076-911X
Le Thi Ngoc Quynh Department of Biotechnology, Thuyloi University, Hanoi City 122300, Vietnam https://orcid.org/0009-0006-2839-3776
Tran Thi Thanh Huyen Faculty of Biology, Hanoi National University of Education, Hanoi City 122300, Vietnam https://orcid.org/0000-0002-5351-6401
Nguyen Quoc Trung Faculty of Biotechnology, Vietnam National University of Agriculture, Gia Lam District, Hanoi City 122300, Vietnam https://orcid.org/0000-0003-1123-3419
Dong Huy Gioi Faculty of Biotechnology, Vietnam National University of Agriculture, Gia Lam District, Hanoi City 122300, Vietnam https://orcid.org/0000-0003-4977-3576
Tran Van Tien Faculty of Social Management, National Academy of Public Administration, Hanoi City 122300, Vietnam https://orcid.org/0000-0001-7407-318X
Phi Bang Cao Faculty of Natural Sciences, Hung Vuong University https://orcid.org/0000-0001-9374-3839

DOI:

https://doi.org/10.11594/jtls.14.01.10

Keywords:

Gene identification, trehalose-6-phosphate synthase, trehalose-6-phosphate phosphatase, peanut (Arachis hypogaea)

Abstract

Plant trehalose has been regarded to play a key role in various biological processes during the growth and development stages. Trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) are two important enzymes for the synthesis of plant trehalose. Up till now, the TPS and TPP gene families have been identified and characterized in numerous higher plant species, but are rarely recorded in peanuts (Arachis hypogaea). In this study, a comprehensive search was performed to identify all putative TPS and TPP proteins in the peanut genome using Arabidopsis TPS and TPP proteins as queries. We then analyzed the characteristics of TPS and TPP members, including physic-chemical parameters, subcellular localization, phylogeny relationships, gene duplication, and expression patterns by various computational tools. As a result, a total of 17 ArahyTPS and 15 ArahyTPP genes were identified and annotated in the peanut genome, which was expanded by segmental duplication events. Our Neighbor-Joining based phylogenetic tree indicated that the ArahyTPS and ArahyTPP proteins could be categorized into three and two major branches. Gene structures and protein features analysis exhibited that the ArahyTPS and ArahyTPP proteins shared high structural and functional similarities. Based on previous RNA-Seq datasets, a majority of the ArahyTPS and ArahyTPP genes were found to specifically express in at least one major organ/tissue during the growth and development. This work will not only lead to a solid foundation on reveal the potential roles of ArahyTPS and ArahyTPP gene families in peanuts but also provide evidence to related trehalose research in other higher plant species.

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Identification of Two Enzymes for Trehalose Synthesis and Their Potential Function in Growth and Development in Peanut (Arachis hypogaea)

Genome Analysis of the Encoding Trehalose Synthesis Enzymes in Peanut

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