Alterations in Blaood Profiles and Spleen Tissue Following Purple Sweet Pota-to Anthocyanin Extract Treatment in Restraint-Stressed Mice

Blood Profiles and Spleen Tissue Following Purple Sweet Potato Anthocyanin Extract Treatment


  • Nia Kurnianingsih Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia Smart Molecule and Natural Genetic Resources Research Centre, Universitas Brawijaya Malang, 65145, Indonesia
  • Dian Artamevia Bachelor Program of Medicine, Faculty of Medicine, Universitas Brawijaya, 65145, Indonesia
  • Dian Hasanah Department of Physiology, Faculty of Medicine, Universitas Brawijaya, 65145, Indonesia
  • Novita Titis Harbiyanti Department of Physiology, Faculty of Medicine, Universitas Brawijaya, 65145, Indonesia
  • Retty Ratnawati Department of Physiology, Faculty of Medicine, Universitas Brawijaya, 65145, Indonesia



anthocyanin, blood, caspase, stress, purple potato, spleen


Stress is associated with broad changes in blood and immune cell dysfunction, which are correlated with the spleen as a secondary lymphoid organ. Anthocyanin, a plant bioactive compound from purple sweet potatoes, has previously been reported to have a beneficial effect on stress response behavior. However, the limited studies evaluated anthocyanin's effect on blood profiles and spleen tissue under stress. Therefore, this study aimed to explore the effect of total anthocyanin extract (ANC) from purple sweet potatoes (PSP) on the blood profiles and spleen tissue of restraint stressed mice. A total of 20 male adult BALB/c mice were divided into control (CTRL), stress (STR), stress + ANC 10 mg/kgBW (STR+ANC10), stress + ANC 20 mg/kgBW (STR+ANC20), and stress + ANC 40 mg/kgBW (STR+ANC10). Restraint stress was applied two hours/day for 14 days. An automatic blood analyzer was used for blood profiling. Immunoblotting of caspase-3 and hematoxylin-eosin-stained histological slides were observed for spleen tissue analysis. Combination of STR and ANC treatment reduced the erythrocyte count, hemoglobin level, and leucocyte count. The ANC-treated groups had a higher percentage of lymphocytes and a lower percentage of granulocytes. The expression of caspase-3 declined among ANC-treated groups. Spleen extramedullary erythropoiesis, vacuolization, and white pulp involution were demonstrated in stress and ANC-treated animals. In summary, restraint stress modified the blood cell profiles and spleen tissue in the mice model. Further research is necessary to evaluate the mechanism of ANC during stress on blood cells and splenic tissue repair enhancement.

Author Biography

Nia Kurnianingsih, Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia Smart Molecule and Natural Genetic Resources Research Centre, Universitas Brawijaya Malang, 65145, Indonesia

Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia


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