The Effect of Various Photoperiodic Conditions and Zn2+ Concentrations on Growth Rate and Metabolite Content in Euglena sp: Effect of Photoperiod and Zn2+ on Euglena sp.

Eko Agus Suyono; Arief Budiman; Rejeki Siti Ferniah; Adam Astiti; Deviko Mardyansah; Fitri Natalia; Maya Cindiati; Khusnul Qonita Maghfiroh; Tia Erfianti; Istini Nurafifah; Ria Amelia; Dedy Kurnianto; Brilian Ryan Sadewo; Revata Maggandari

doi:10.11594/jtls.14.02.04

Authors

Eko Agus Suyono Universitas Gadjah Mada
Arief Budiman Universitas Gadjah Mada
Rejeki Siti Ferniah Universitas Diponegoro
Adam Astiti Universitas Gadjah Mada
Deviko Mardyansah Universitas Gadjah Mada
Fitri Natalia Universitas Gadjah Mada
Maya Cindiati Universitas Gadjah Mada
Khusnul Qonita Maghfiroh Universitas Gadjah Mada
Tia Erfianti Universitas Gadjah Mada
Istini Nurafifah Universitas Gadjah Mada
Ria Amelia Universitas Gadjah Mada
Dedy Kurnianto National Research and Innovation Agency
Brilian Ryan Sadewo Universitas Gadjah Mada
Revata Maggandari Universitas Gadjah Mada

DOI:

https://doi.org/10.11594/jtls.14.02.04

Keywords:

Euglena sp, Growth, Metabolites, Photoperiodic, Zn2

Abstract

The application of Euglena as a carbon capture organism has generated considerable interest among scientists. Through the photosynthesis process, many kinds of metabolites are produced by Euglena, such as lipids, proteins, and pigments. Due to the metabolites produced by Euglena, it is vital to optimize the carbon capture ability and cell growth rate by adding Zn²⁺ content and giving photoperiodic into Euglena culture. The purpose of this study is to identify the optimal photoperiod and Zn²⁺ concentration to increase the growth rate, biomass, and metabolite content of Euglena sp. This study is a laboratory experiment involving the cultivation of Euglena sp. in various photoperiod cycles (light:dark), namely 24:0, 12:12, 14:10, and 16:8. In addition, Euglena sp. was also cultivated using different concentrations of Zn²⁺ (0 ppm, 5 ppm, 10 ppm, and 15 ppm). The growth of Euglena sp. was monitored for 18 days before being harvested every three days to measure the research parameters, including primary and secondary metabolites. The results showed that the photoperiod treatment and various concentrations of Zn²⁺ had a significant impact (P<0.05) on the growth rate, biomass, lipid, carbohydrate, protein, chlorophyll, and carotenoid levels of Euglena sp.

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