Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L.

Nina Artanti, Muhammad Hanafi, Rina Andriyani, Vienna Saraswaty, Linar Zalinar Udin, Puspa D Lotulung, Ken Ichi Fujita, Yoshinosuke Usuki


Hedyotis corymbosa, with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine.  The ethanol extract of H. corymbosa L. shows inhibitory activity to human YMB-1 breast cancer cell line with an IC50 of 6.51 mg/ml.  The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 mg/ml.  To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 mg/ml, respectively.  Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.

Full Text:



Ahmad, R., Ali, A.M., Israf, D.A., Ismail, N. H. Saari, K., and Lajis, N.H., 2004. Life Sciences, 76,1953-1964.

Liou, W-C., Lin, Y-C., Lin Y-M. and Chen, F-C. 1979. Chemistry (Taipei) (3), 72-73

Ho, T-I, Chen, G-P, Lin, Y-C, and Chen F-A, 1986, Phytochemistry 25(8), 1988-1989

Jiang, W. Kuang L-S, Hou, A-J, Qian, M. and L, J-Z., 2007, Helvetica Chimica Acta. 90 (7), 1296-1301.

Q. M. Yuan, H.W. Yang, F. G. Zhang, X.Wang, B. Z. Zheng, J. Yunnan Univ. 2004, 26 (suppl.), 171.

Y. H. Chen, F. R. Chang, C. C.Wu, M. H. Yen, C. C. Liaw, H. C. Huang, Y. H. Kuo, Y. C.Wu. 2006. Planta Med., 72, 75;

J. F. Zhao, Q. M. Yuan, X. D. Yang, H. B. Zhang, L. Li, . 2005. Helv. Chim. Acta, 88, 2532-2536

R. Ahmad, K. Shaari, N. H. Lajis, A. S. Hamzah, N. H. Ismail, M. Kitajima, 2005. Phytochemistry, 66, 1141.

Jiangsu College of New Medicine, in HA Dictionary of the Traditional Chinese MedicinesI, Shanghai Scientific and Technical Publishers, Shanghai, 1977, p. 535.

Sini Sadasivan, P.G. Latha, J.M. Sasikumar , S. Rajashekaran, S. Shyamal, V.J. Shine, 2006. J. Ethnopharmacology, 106, 245–249.

Rina Andriyani*, Chandra Risdian, and Zalinar Udin. 2011. Indonesian Journal of Cancer Chemoprevention (IJCC), 2(1):182-186.

Jae Hyeok Lee, Chung Hwan Ku, Nam-In Baek, Sung-Hoon Kim, Hee Wook Park, and Dae Keun Kim., 2004, Arch. Pharm. Res., 27 (1), 40-43.

Jiang –Nan Peng, Xiao-Zhang Feng, and Xiao-Tian Liangnan Peng., 1998, 47 (8), 1657-1659.


Copyright (c) 2015 Journal of Tropical Life Science