Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L.

Nina Artanti, Muhammad Hanafi, Rina Andriyani, Vienna Saraswaty, Linar Zalinar Udin, Puspa D Lotulung, Ken Ichi Fujita, Yoshinosuke Usuki

Abstract


Hedyotis corymbosa, with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine.  The ethanol extract of H. corymbosa L. shows inhibitory activity to human YMB-1 breast cancer cell line with an IC50 of 6.51 mg/ml.  The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 mg/ml.  To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 mg/ml, respectively.  Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.

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DOI: http://dx.doi.org/10.11594/jtls.05.02.06

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