The Effectiveness of Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo (Bubalus bubalis)

Adding Different Sucrose in Tris-Egg Yolk Diluent on the Sperm Fertility of Sexing Swamp Buffalo

Authors

  • Yendraliza Yendraliza UIN Suska Riau
  • Allif Bukhori Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau
  • Edi Erwan Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau
  • Ria Sari Gail Sianturi Indonesian Research Institute for Animal Production - PO Box 221 Bogor 16002
  • Diana Andrianita Kusumaningrum Indonesian Research Institute for Animal Production - PO Box 221 Bogor 16002
  • Muhamad Rodiallah Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau
  • Zumarni Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

DOI:

https://doi.org/10.11594/jtls.13.02.17

Keywords:

Acrosome, Intact membrane plasm, Motility, Sexing, Spermatozoa, Sucrose

Abstract

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets to determine the effectiveness of adding sucrose to tris-egg yolk diluent on the sperm quality of the swamp buffaloes produced by sexing with the egg albumin method. Semen was collected once a week for 12 consecutive weeks with an artificial vagina from three male buffaloes at the Indonesian Center for Animal Research and Development (ICARD) in April - June 2021. Semen was separated by sedimentation using 10% and 30% egg albumins. Semen from sexing was divided into four diluent treatments, namely 20% tris-egg yolk (TEY), TEY+0% sucrose, TEY+0.2% sucrose, TEY+0.3% sucrose, and TEY+0.4% sucrose. Parameters measured included: motility, viability, intact plasm membrane, and intact acrosome cap of buffalo sperm. The results showed the motility of X sperm: 43.75-47.5%, Y sperm: 40-45%, the viability of X sperm: 78.25-79.75% and Y sperm: 77.25-79.75%, intact plasm membrane of X sperm: 70.5-71.5% and Y sperm: 70.5-71%, and viable acrosome-intact for X sperm: 79-80% and Y sperm: 78.75-79.5%. The addition of sucrose up to 0.4% into tris-egg yolk diluent was proven can maintain motility, viability, and integrity of cell membrane and acrosome of spermatozoa of water buffalo that was sexed with egg albumin.

Author Biographies

  • Allif Bukhori, Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

    Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

  • Edi Erwan, Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

    Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

  • Ria Sari Gail Sianturi , Indonesian Research Institute for Animal Production - PO Box 221 Bogor 16002

    Indonesian Research Institute for Animal Production - PO Box 221 Bogor 16002

  • Diana Andrianita Kusumaningrum, Indonesian Research Institute for Animal Production - PO Box 221 Bogor 16002

    Indonesian Research Institute for Animal Production - PO Box 221 Bogor 16002

  • Muhamad Rodiallah, Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

    Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

  • Zumarni, Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

    Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, H.R. Soebrantas KM. 15 Street, Pekanbaru, 28293, Riau

References

Statistics Indonesia (BPS). (2021). Buffalo Population Indonesia. Jakarta.

Yendraliza, Yuliana E, Rodiallah M, Zumarni (2019) Kualitas Semen Kerbau pada Waktu Ekuilibrasi dan Inkubasi yang Berbeda dalam Larutan Hipoosmotic Swelling TEST. Agripet, 19(1): 22–30. DOI: 10.17969/agripet.v19i1.13191.

Singh I, Balhara AK (2016) New approaches in buffalo artificial insemination programs with special reference to India. Theriogenology 86(1): 194–199. DOI: 10.1016/j.theriogenology.2016.04.031.

Afiati F (2004) Proporsi dan Karakteristik Spermatozoa X dan Y Hasil Separasi Kolom Albumin. Media Peternakan 27(1): 14–17. DOI: 10.5398/medpet.v27i1.695.

Maulana T, Said S, Arifiantini RI, Setiadi MA (2019) Sex sorting sperm of sumba Ongole bulls by using snakehead fish (Channa striata) albumin extract. Journal of the Indonesian Tropical Animal Agriculture 44(1): 106–113. DOI: 10.14710/jitaa.41.1.106-113.

Situmorang P, Sianturi RG, Kusumaningrum DA, Ross R, Maidaswar M (2013) Female calves born resulted from an artificial inseminatioan (AI) using sexed sperm seperated with egg albumin coloum. Jurnal Ilmu Ternak Dan Veteriner 18(3): 185–191. DOI: 10.14334/jitv.v18i3.320.

Garner DL, Hafez ESE Spermatozoa and Seminal Plasm. In Reproduction in Farm Animals Lippincott Williams & Wilkins. DOI: 10.1002/9781119265306.ch7.

Andrabi S (2009) Factors Affecting the Quality of Cryopreserved Buffalo (Bubalus bubalis) Bull Spermatozoa. Reproduction in Domestic Animals 44(3): 552–569. DOI: 10.1111/j.1439-0531.2008.01240.x.

Holt WV (2000a). Basic aspects of frozen storage of semen. Animal Reproduction Science 62(1): 3–22. DOI: 10.1016/S0378-4320(00)00152-4.

Bearden HJ, Fuquay JW (2004) Applied Animal Reproduction. 6th edition. Reston Publishing Company, INC. https://www.pearson.com/us/higher-education/program/Bearden-Applied-Animal-Reproduction-6th-Edition/PGM62964.html.

Akhter S, Rakha BA, Ansari MS, Iqbal S, Khalid M (2018) Evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology 119: DOI: 10.1016/j.theriogenology.2018.06.019.

Souhoka DF, Matatula MJ, Mesang-Nalley WM, Rizal M (2009) Role of Lactose in Maintaining Viability of Peranakan Etawah Goat Spermatozoa Preserved with Pri-angan Ram Seminal Plasm. Journal Veteriner 10: 135-142.

Woelders H, Matthijs A, Engel B (1997) Effects of Trehalose and Sucrose, Osmolality of the Freezing Medium, and Cooling Rate on Viability and Intactness of Bull Sperm after Freezing and Thawing. Cryobiology 35: 93–105.

Rizal M, Herdis, Yulnawati, Maheswari H (2007) The Quality Enhancement of Epididymal Spermatozoa of spotted Buffalo Cryopreserving with various Sucrose Concentrations. Journal Veteriner 8: 188-193.

Yulnawati, Herdis (2009) The Quality of Garut Ram Liquid Semen in Tris Egg Yolk Extender to the Sucrose Supplementation. JITV 14: 45-49.

Akhter S, Ansari MS, Rakha BA et al. (2010) Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell® extender. Theriogenology 74(6): 951–955. DOI: 10.1016/j.theriogenology.2010.04.024.

Kusumaningrum DA, Sianturi RG (2018) Pengaruh Waktu Pemisahan Spermatozoa Terhadap Kualitas Perma Kerbau Hasil Sexing. JNP 5: 368–374. http://jnp.fapet.unsoed.ac.id/index.php/psv/article/view/60.

Susilawati T. Spermatology. Universitas Brawijaya (UB) Press Malang, Malang.

Jeyendran RS, Van Der Ven HH, Perez-Pelaez M et al. (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70(1): 219–228. DOI: 10.1530/jrf.0.0700219.

Bhalakiya N, Haque N, Patel D et al. (2018) Sperm sex-ing and its application in livestock sector. Int. J. Curr. Mirobial. App. Scie Special Issues 7: 259-272.

Sianturi R, Purwantara B, Supriatna I, Amrozi, Situ P (2012) Pengaruh Glutation dan Penggantian Plasm Semen Kerbau dengan Plasm Semen Sapi terhadap Kualitas Semen Beku Kerbau Rawa (Bubalus bubalis). Jurnal Ilmu Ternak Dan Veteriner 17(3): 169–178.

Herbowo MT, Arifiantini RI, Karja NWK, Sianturi RG (2019) Cryopreservation of swamp buffalo semen in skim milk yolk-based diluent with two different cryoprotectants. Tropical Animal Science Journal 42(1): 13–18. DOI: 10.5398/tasj.2019.42.1.13.

Ghodasara SN, Gajbhiye PU, Ahlawat AR, Murthy KS (2016) Evaluation Of Fresh Semen Quality And Predicting The Number Of Frozen Semen Doses In Jaffrabadi Buffalo Bull. Buffalo Bulletin 35(1): 65–72.

Bhave K, Koilpillai TPJ, Ragothaman V et al. (2020) Semen production and semen quality of indigenous buffalo breeds under hot semiarid climatic conditions in India. Tropical Animal Health and Production 52(5): 2529–2539. DOI: 10.1007/s11250-020-02284-9.

Standardisasi Nasional Indonesia (SNI). (2008) SNI 4869.2-2008 Semen Beku Kerbau Semen Beku Kerbau. https://id.scribd.com/document/345026647/SNI-4869-2-2008-Semen-Beku-Kerbau.

Jainudeen MR, Hafez ESE (2016) Cattle and Buffalo. In Reproduction in Farm Animals. Lippincott Williams & Wilkins. DOI: 10.1002/9781119265306.ch11.

Bhakat M, Mohanty TK, Raina VS et al. (2011) Effect of Age and Season on Semen Quality Parameters in Sahiwal Bulls. Trop. Anim. Health Pro 43: 1161-1168.

Eriani K, Azhar A, Ihdina M et al. (2018) Quality enhancement of aceh swamp buffalo (bubalus bubalis) frozen semen by supplementing ß-carotene. Tropical Animal Science Journal 41(1): 1–7. DOI: 10.5398/tasj.2018.41.1.1.

El-Sisy GA, Shahba MI, El-Sheshtawy RI (2016) Freezability of buffalo semen with TRIS extender enriched with disaccharides (trehalose or sucrose) and different glycerol concentrations. Asian Pacific Journal of Reproduction 5(5): 416–418. DOI: 10.1016/j.apjr.2016.07.007.

Holt WV (2000b) Basic aspects of frozen storage of semen. Animal Reproduction Science 62(1–3): 3–22. DOI: 10.1016/S0378-4320(00)00152-4.

Watson PF (2000) The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science 60: 481–492. DOI: 10.1016/S0378-4320(00)00099-3.

Boro P, Naha BC, Madkar A, Prakash C (2016) Sexing of semen in bulls : A mini review. International Journal of Applied Research 2(4): 460–462.

SNI (Indonesian National Standardization) (2017) Frozen sperm Part 2 Buffalo Sperm. BSN. Jakarta.

Uchida T, Nagayama M, Shibayama T, Gohara K (2007) Morphological investigations of disaccharide molecules for growth inhibition of ice crystals. Journal of Crystal Growth 299(1): 125–135. DOI: 10.1016/j.jcrysgro.2006.10.261.

Farstad W (1996) Semen cryopreservation in dogs and foxes. Animal Reproduction Science 42(96): 251–260.

Bergeron A, Brindle Y, Blondin P, Manjunath P (2007) Milk Caseins Decrease the Binding of the Major Bovine Seminal Plasm Proteins to Sperm and Prevent Lipid Loss from the Sperm Membrane During Sperm Storage 1. Biology of Reproduction 77(April): 120–126. DOI: 10.1095/biolreprod.106.058248.

Akhter S, Rakha BA, Ansari MS et al. (2017) Evaluation of quail and turkey egg yolk for cryopreservation of Nili-Ravi buffalo bull semen. Theriogenology 87: DOI: 10.1016/j.theriogenology.2016.09.002.

Asma-ui-Husna A, Awan MA, Mehmood A et al. (2017) Sperm sexing in Nili-Rapvi buffalo through modified swim up Validation using SYBR green real-time PCR. Animal Reproduction Science 182: 69–76. DOI: 10.1016/j.anireprosci.2017.04.011.

Balao da Silva CM, Ortega Ferrusola C, Morillo Rodriguez A et al. (2013) Sex sorting increases the permeability of the membrane of stallion spermatozoa. Animal Reproduction Science 138(3–4): 241–251. DOI: 10.1016/j.anireprosci.2013.02.021.

Dorado J, Muñoz-Serrano A, Hidalgo M (2010) The effect of cryopreservation on goat semen characteristics related to sperm freezability. Animal Reproduction Science 121(1–2): 115–123. DOI: 10.1016/j.anireprosci.2010.04.182

Fahy GM (1984) Vitrification as an Approach to Cryopreservation. Cryobiology 21: 407–426.

Qadeer S, Khan MA, Ansari MS et al. (2015) Efficiency of antifreeze glycoproteins for cryopreservation of Nili-Ravi (Bubalus bubalis) buffalo bull sperm. Animal Reproduction Science 157: 56–62. DOI: 10.1016/j.anireprosci.2015.03.015.

Asma-ul-Husna, Awan MA, Mehmood A et al. (2017) Sperm sexing in Nili-Ravi buffalo through modified swim up: Validation using SYBR®green real-time PCR. Animal Reproduction Science 182. DOI: 10.1016/j.anireprosci.2017.04.011.

Vishwanath R, Moreno JF (2018) Review: Semen sexing - Current state of the art with emphasis on bovine species. Animal 12(s1): s85–s96. DOI: 10.1017/S1751731118000496.

Srivastava N, Jerome A, Srivastava SK et al. (2013) Bovine seminal PDC-109 protein: An overview of biochemical and functional properties. Animal Reproduction Science 138(1–2): 1–13. DOI: 10.1016/j.anireprosci.2013.02.008.

Aisen EG, Alvarez HI, Venturino A, Garde JJ (2000) Effect of Trehalose and Edta on Cryoprotective Action of Ram Semen Diluents. Theriogenology 53: 1053–1061. https://doi-org10.1016/S0093-691X(00)00251-X./.

Manjunath P (2012) New insights into the understanding of the mechanism of sperm protection by extender components. Animam Reproduction 9(4): 809–815. http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v9n4/p809-815.pdf.

Aitken RJ, Jones KT, Robertson SA (2012) Reactive oxygen species and sperm function-in sickness and in health. Journal of Andrology 33(6): 1096–1106. DOI: 10.2164/jandrol.112.016535.

Amirat, L., Anton, M., Tainturier, D., Chatagnon, G., Battut, I., & Courtens, J. L. (2005). Modifications of bull spermatozoa induced by three extenders: Biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing. Reproduction, 129(4), 535–543. DOI: 10.1530/rep.1.00011.

Oldenhof H, Gojowsky M, Wang S et al. (2013) Osmotic stress and membrane phase changes during freezing of stallion sperm: Mode of action of cryoprotective agents. Biology of Reproduction 88(3): 1–11. DOI: 10.1095/biolreprod.112.104661.

Rasad SD, Solihati N, Winangun K (2021) The quality of Etawah crossbreed sperm after sexing with different combination of Bovine Serum Albumin concentrations. IOP Conference Series: Earth and Environmental Science 888(1). DOI: 10.1088/1755-1315/888/1/012025.

Awan MA, Akhter S, Husna AU et al. (2018) Antioxidant activity of Nigella sativa Seeds Aqueous Extract and its use for cryopreservation of buffalo spermatozoa. Andrologia 50(6). DOI: 10.1111/and.13020.

Yildiz C, Aksoy AK, Tekeli T (2000) Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing. Theriogenology 54(4): 579–585.

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Published

2023-06-07

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Vol. 13 No. 2 (2023)

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