Effect of microbial inoculant on the oviposition and completion of life cycle of Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) infesting rabbit carcasses

Effect of microbial inoculant on Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart)


  • Naji Arafat Mahat Department of Chemistry, Faculty of Science, University Teknologi Malaysia, 81310 Skudai, Johor
  • Nur Amalina Omar Department of Chemistry, Faculty of Science, University Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
  • Heo Chong Chin Faculty of Medicine, Universiti Teknologi Mara, Sungai Buloh, Selangor, Malaysia
  • Mohammad Rahim Kamaluddin Centre for Research in Psychology and Human Well-Being, Faculty of Social Sciences and Humanities, Department of Universiti Kebangsaan Malaysia, 43600, Malaysia
  • Nor Wajihan Muda Department of Chemistry, Faculty of Science, University Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.




Chrysomya megacephala, Chrysomya rufifacie, forensic science, Forensic entomology, Microbial inoculant, Post mortem interval, Oviposition and development


This research was conducted to assess the influence of microbial inoculant (EM.1®) on the initial oviposition and developmental pattern of two prevalent necrophagous flies (Chrysomya megacephala and Chrysomya rufifacies) in Malaysia. Nine rabbit carcasses were equally divided into control and two treated groups. The control carcasses were sprayed with deionized water (about 100 mL), whereas the two other treated groups were individually sprayed with two concentrations of EM.1® at 1:500 and 1:100, respectively. Results revealed that the single application of EM.1® on both treated carcasses did not statistically impede oviposition by both necrophagous species, as well as their subsequent developmental patterns (P > 0.05) when compared with that of controls. Therefore, estimating the minimum post-mortem interval (mPMI) using empirical baseline data for the control animal model would remain appropriate for estimating mPMI in cases involving carcasses with a single application of EM.1®

Author Biography

Nor Wajihan Muda, Department of Chemistry, Faculty of Science, University Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.

Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti
Teknologi Malaysia, Johor Bahru, 81310, Johor, Malaysia.


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