The Selection and the Growth Condition Optimization of Ethanol‐Producing Microbes Isolated from Ragi Tapai
Selection and the Growth Condition Optimization of Ethanol‐Producing Microbes
Keywords:Cassava tapai, Ethanol-producing microbe, Optimization condition, Ragi Tapai
Ethanol is a biofuel produced from renewable resources, which potentially plays an important role in solving future fuel problems. This study aimed to select the highest ethanol-producing isolate from candidates obtained from previously isolated candidates from ragi and cassava tapai. The selection process was conducted in 2 stages, namely: 1) Selection of the highest ethanol-producing isolate from seven isolate candidates using PYG media containing peptone, yeast extract, and glucose at 0.75%, 0.75%, and 15%, respectively and was followed by: 2) Optimization of the growth conditions of the highest ethanol-producing isolate, which was conducted at various temperatures of 27, 30, 33, 35, 37, and 40°C with the combination of various pH of 3.5, 4.5, 5.5, and 6.5. The experimental results showed that the R5I3 isolate was the highest ethanol-producing performance isolate, which yielded approximately 4.69±0.25% (v/v). Following the temperature and pH optimization of the fermentation processes, the optimum growth conditions were at 35°C and pH 5.5, where the ethanol produced was increased to 8.63 ± 0.04% (v/v). With these results, this new strain has the potential to be used in bioethanol production processes and other industrial applications.
Mellicha SV, Gunam IBW, Antara NS, Arnata IW (2021) Production of bioethanol from wild cassava crude starch (Manihot glaziovii Muell. Arg) using different mi-crobial types and fermentation times. IOP Conf. Series: Earth and Environmental Science 913(2021)012032 doi:10.1088/1755 -1315/913/1/012032.
Arnata IW, Gunam IBW, Anggreni AAMD, Wijaya IMM, Sartika D (2021) Utilization of solid tapioca waste for bioethanol production by co-fermentation of baker’s and tapai yeast. IOP Conf. Series: Earth and Environmen-tal Science724(2021)012058 doi:10.1088/1755-1315/724/1/012058
Gunam IBW, Sitepu A, Antara NS, Triania IGAL, Arna-ta IW, Setiyo Y (2021) bacterial desulfurization of diben-zothiophene by Pseudomonas sp. strain KWN5 immobi-lized in alginate beads. Jurnal Teknologi 83(2): 107–115doi: 10.11113/jurnalteknologi.v83. 15080.
Gunam IBW, Dewi IAPK, Antara NS, Anggreni AAMD, Setiyo Y (2021) Bioethanol production from sugarcane bagasse by Saccharomyces cerevisiae ATCC 9763 immobilized in Na-alginate. IOP Conf. Series: Earth and Environmental Science 824(2021)012054doi:10.1088/1755-1315/82 4/1/012054.
Mohseni M, Ebrahimi H, Chaichi MJ (2013) Isolation and optimization of ethanol producing bacteria from natu-ral environments of Mazandaran province in Iran. Journal of Genetic Resources 1(1): 35–44 doi: 10.22080/jgr.2015.1120.
Pranata WS, Suryadarma P, Mangunwidjadja D (2018) Glycerol utilization as substrate for ethanol production in Escherichia coli recombinant under an aerobic condition. The Journal of Tropical Life Science (8(1): 81 – 86 doi: 10.11594/jtls.08.01.14
Soetarto ES, RN Putri (2016) Bioethanol fermentation from sugarcane bagasse using ragi tape. AIP Conference Proceedings 1744, 020020 doi:10.1063/1.4953494
Gunam IBW, Antara NS, Anggreni AAMD, Setiyo Y, Wiguna IPE, Wijaya IMM, Putra IWWP (2019) Chemi-cal pretreatment of lignocellulosic wastes for cellulase production by Aspergillus niger FNU 6018. AIP Confer-ence Proceedings 2155,020040 doi:10.1063/1.5125544
Azmi AS, Ngoh GC, Mel M, Hasan M (2010) Ragi tapai and Saccharomyces cerevisiae as potential coculture in viscous fermentation medium for ethanol production. Af-rican Journal of Biotechnology 9(42): 7122-7127 doi: 10.5897/AJB10.933.
Gunam IBW, Sujana IGA, Wijaya IMM, Setiyo Y, Putra IWWP, Suriati L (2021). Isolation and selection of amyl-ase-producing microbes isolated from ragi tape and cas-sava tape available on the markets. IOP Conf. Series: Earth and Environmental Science 913(2021)012041 doi:10.1088/1755-1315/913/1/012041
Sujaya IN, Mikumo D, Orikasa Y, Urashima T, Oda Y (2011) Baking properties of Saccharomyces cerevisiae strains derived from brem, a traditional rice wine from Bali. Food Science and Technology Research, 17 (4): 369–373 doi: 10.3136/fstr.17.369.
Buraimoh OM, Ogunyemi AK, Oyegbite IE, Amund OO, Ilori MO (2020) Potentials of hydrocarbon utiliza-tion by wood-digesting bacteria isolated from the Lagos Lagoon, Nigeria. The Journal of Tropical Life Science 10 (1): 1–10. doi: 10.11594/jtls.10.01.01
Azmi AS, Hasan M, Mel M, Ngoh GC (2009) Single step bioconversion of starch to bioethanol by the cocul-ture of ragi tapai and Saccharomyces cerevisiae. Chemical engineering transactions 18: 557–562. doi:10.3303/CET0918090
Sonwani RK, Pandey S, Yadav SK, Giri BS, Katiyar V, Singh RS, Rai BN (2021) Construction of integrated sys-tem for the treatment of Acid orange 7 dye from wastewater: Optimization and growth kinetic study. Bio-resource Technology 337(2021) 125478:1–7 doi:10.1016/j.biortech.2021.125478.
Hashem M, Alamri SA, Asseri TAY, Mostafa YS, Lyberatos G, Ntaikou I (2021) On the optimization of fermentation conditions for enhanced bioethanol yields from starchy biowaste via yeast co-cultures. Sustainability (13)1890: 1–13 doi:10.3390/ su13041890.
Hashem M, Asseri TYA, Alamri SA (2019) Feasibility and sustainability of bioethanol production from starchy restaurants’ bio-wastes by new yeast strains. Waste Bio-mass Valorization 10:1617–1626 doi:10.1007/s12649-017-0184-7.
Ma’As MF, Ghazali HM, Chieng S (2020) Bioethanol production from Brewer’s rice by Saccharomyces cere-visiae and Zymomonas mobilis: evaluation of process ki-netics and performance. Energy Sources, Part A: Recov-ery, Utilization, and Environmental Effects 1–14 doi:10.1080/15567036.2020.-1815901.
Khan RA, Nawaz A, Ahmed M, Khan MR, Azam FD, Ullah S, Sadullah F, Ahmad A, Shah MS, Khan N (2012) Production of bioethanol through enzymatic hy-drolysis of potato. African Journal of Biotechnology 11(25): 6739–6743 doi:10.5897/AJB11. 2791.
Lai YT, Cheng KC, Lai CN, Lai YJ (2019) Isolation and identification of aroma producing strain with esterification capacity from yellow water. PLoS ONE 14(2) doi:10.1371/journal.pone.0211356.
Chang YH, Chang KS, Chen CY, Hsu CL, Chang TC, Jang HD (2018) Enhancement of the efficiency of bioeth-anol production by Saccharomyces cerevisiae via gradu-ally batch-wise and fed-batch increasing the glucose con-centration. Fermentation 2018(4) doi:10.3390/fermentation4020045.
Ogbonda KH, Kiin-Kabari DB (2013) Effect of tempera-ture and pH on ethanol production by a Blastomyces spe-cies isolated from the intestine of oil palm weevil (Rhyn-chophorus palmarum, coleoptera). African Journal of Biotechnology 12(6): 588-591, doi: 10.5897/AJB10.147.
Napitupulu AMM, Suhendra L, Gunam IBW (2021) Effect of Saccharomyces cerevisiae ATCC 9763 concen-tration and fermentation time on bioethanol content from corn stover crude cellulose substrate. IOP Conf. Series: Earth and Environmental Science 913(2021)012026 doi:10.1088/1755-1315/913/1/012026.
Sarungallo RS, Mellolo A, Melawaty L, Djonny M (2020) The effect of boiler size of distillation to the con-centration of bioethanol and yield from fermentation of sweet sorghum juice. IOP Conference Series: Materials Science and Engineering 885(1): 12–14 doi:10.1088/1757-899X/885/1/ 012014.
Efeovbokhan, V. E., L. Egwari, E. E. Alagbe, J. T. Adeyemi and O.S. Taiwo. 2019. Production of bioethanol from hybrid cassava pulp and peel using microbial and acid hydrolysis. BioResources. 14(2):2596–2609 doi:10.15376/biores.14.2.2596.2609
Rusydi AF (2018) Correlation between conductivity and total dissolved solid in various type of water: A review. IOP Conf. Series: Earth and Environmental Science 118(2018)012019 doi:10.1088/1755-1315/118/1/012019.
Choi, Gi-Wook, Um HJ, Kim M, Kim Y, Kang HW, Chung BW, and Kim YH (2010) Isolation and characteri-zation of ethanol-producing Schizosaccharomyces pombe CHFY0201. Journal of Microbiology and Biotechnology 20(4): 828–834 doi:10.4014/ jmb.0910.10003
Narendranath NV, Thomas KC, Ingledew WM (2001) Effects of acetic acid and lactic acid on the growth of Saccharomyces cerevisiae in a minimal medi-um. Journal of Industrial Microbiology and Biotechnolo-gy 26(3): 171–177. doi:10.1038/sj.jim.7000090.
Esmaeili H Keikhosro K (2018) Optimization of fermen-tation conditions for efficient ethanol production by Mu-cor hiemalis. Turkish Journal of Biochemistry 43(6): 587-594. doi:10.1515/tjb-2017-0290.
Copyright (c) 2022 Journal of Tropical Life Science
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Journal of Tropical Life Science is licensed under Creative Commons Attribution-NonCommercial 4.0 International License