The Selection and the Growth Condition Optimization of Ethanol‐Producing Microbes Isolated from Ragi Tapai

Selection and the Growth Condition Optimization of Ethanol‐Producing Microbes

Authors

  • Ida Bagus Wayan Gunam Udayana University
  • I Gede Arya Sujana Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia
  • I Made Mahaputra Wijaya Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia
  • Yohanes Setiyo Department of Agricultural and Biosystem Engineering, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran, Badung Bali 80361, Indonesia
  • I Wayan Arnata Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia
  • I Wayan Wisma Pradnyana Putra Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia

DOI:

https://doi.org/10.11594/jtls.13.02.12

Keywords:

Cassava tapai, Ethanol-producing microbe, Optimization condition, Ragi Tapai

Abstract

Ethanol is a biofuel produced from renewable resources, which potentially plays an important role in solving future fuel problems. This study aimed to select the highest ethanol-producing isolate from candidates obtained from previously isolated candidates from ragi and cassava tapai. The selection process was conducted in 2 stages, namely: 1) Selection of the highest ethanol-producing isolate from seven isolate candidates using PYG media containing peptone, yeast extract, and glucose at 0.75%, 0.75%, and 15%, respectively and was followed by: 2) Optimization of the growth conditions of the highest ethanol-producing isolate, which was conducted at various temperatures of 27, 30, 33, 35, 37, and 40°C with the combination of various pH of 3.5, 4.5, 5.5, and 6.5. The experimental results showed that the R5I3 isolate was the highest ethanol-producing performance isolate, which yielded approximately 4.69±0.25% (v/v). Following the temperature and pH optimization of the fermentation processes, the optimum growth conditions were at 35°C and pH 5.5, where the ethanol produced was increased to 8.63 ± 0.04% (v/v). With these results, this new strain has the potential to be used in bioethanol production processes and other industrial applications.

Author Biographies

  • I Gede Arya Sujana, Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia

    Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia

  • I Wayan Wisma Pradnyana Putra, Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia

    Laboratory of Bioindustry, Faculty of Agricultural Technology, Udayana University, Bukit Jimbaran Badung Bali 80361, Indonesia

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Published

2023-05-25

Issue

Vol. 13 No. 2 (2023)

Section

Articles

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