Antioxidant Activity of Unripe Sapodilla Fruit Extract (Manilkara zapota L.) through Nrf2 and SOD Expression in Type 1 Diabetic Mice
Antioxidant activity of Manilkara zapota extract in T1DM
Keywords:Antioxidant, Free radical, Nrf2, SOD, Manilkara zapota
This research aims to analyze the effect of unripe sapodilla fruit extract on endogenous antioxidant expression in T1DM BALB/c mice and its free radical scavenging activity. Manilkara zapota extract (MzE) is an aqueous extract of unripe sapodilla fruit and was obtained by maceration and freeze-drying process. This study used 25 male BALB/c mice with 7-weeks-old of age. They were divided randomly into five groups (n=5) before treatment. A single high dose (145 mg/kg BW) of streptozotocin (STZ) was intraperitoneally injected to induce type 1 diabetes mellitus (T1DM). MzE was given orally once each day for 14 days. Liver cells were isolated and immunoassay with anti-superoxide dismutase (SOD) and anti-nuclear factor erythroid 2-related Factor 2 (Nrf2), and then the results were analyzed by flow cytometry. Diphenylpicrylhydrazyl (DPPH) assay was performed to analyze free radical scavenging. Data were analyzed statistically with one-way ANOVA (p<0.05). The result showed that the glucose levels in diabetic mice after MzE administration were significantly lower than in the DM group. MzE treatment increased the expression of Nrf2 and SOD in diabetic mice. MzE could scavenge DPPH with the IC50 value obtained at 48.35 μg/mL, while ascorbic acid as a control could scavenge DPPH with the IC50 value at 22.24 μg/mL. The increase in the scavenging activity is in line with the increase in extract concentration. In conclusion, this study revealed that MzE can be an endogenous antioxidant enhancer by improving the expression of Nrf-2, SOD and can inhibit free radicals as an exogenous antioxidant in T1DM.
American Diabetes Association (2010) Diagnosis and Classification of Diabetes Mellitus. Diabetes Care 33 (Supplement_1): S62–S69. doi: 10.2337/dc10-S062.
Volpe CMO, Villar-Delfino PH, dos Anjos PMF, Nogueira-Machado JA (2018) Cellular death, reactive oxygen species (ROS) and diabetic complications. Cell Death & Disease 9 (2): 119. doi: 10.1038/s41419-017-0135-z.
Phaniendra A, Jestadi DB, Periyasamy L (2015) Free Radicals: Properties, Sources, Targets, and Their Implica-tion in Various Diseases. Indian Journal of Clinical Bio-chemistry 30 (1): 11–26. doi: 10.1007/s12291-014-0446-0.
Delmastro MM, Piganelli JD (2011) Oxidative Stress and Redox Modulation Potential in Type 1 Diabetes. Clinical and Developmental Immunology 2011 1–15. doi: 10.1155/2011/593863.
Dworzański J, Strycharz-Dudziak M, Kliszczewska E et al. (2020) Glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity in patients with diabetes melli-tus type 2 infected with Epstein-Barr virus. PLOS ONE 15 (3): e0230374. doi: 10.1371/journal.pone.0230374.
Uruno A, Furusawa Y, Yagishita Y et al. (2013) The Keap1-Nrf2 System Prevents Onset of Diabetes Mellitus. Molecular and Cellular Biology 33 (15): 2996–3010. doi: 10.1128/MCB.00225-13.
Cui W, Min X, Xu X et al. (2017) Role of Nuclear Factor Erythroid 2-Related Factor 2 in Diabetic Nephropathy. Journal of Diabetes Research 2017 1–14. doi: 10.1155/2017/3797802.
Leelarungrayub J (2019) Potential Health Benefits of Thai Seasonal Fruits; Sapodilla and Star Fruit for Elderly Peo-ple. American Journal of Biomedical Science & Research 5 (1): 49–53. doi: 10.34297/AJBSR.2019.05.000873.
Lim WS, M.S. R, Uthumporn U (2017) Development of functional beverage from Sapodilla (Manilkara Zapota L.) fruit. Food Research 2 (2): 163–170. doi: 10.26656/fr.2017.2(2).227.
Shui G, Wong SP, Leong LP (2004) Characterization of Antioxidants and Change of Antioxidant Levels during Storage of Manilkara zapota L. Journal of Agricultural and Food Chemistry 52 (26): 7834–7841. doi: 10.1021/jf0488357.
Wang H, Liu T, Song L, Huang D (2012) Profiles and α-Amylase Inhibition Activity of Proanthocyanidins in Un-ripe Manilkara zapota (Chiku). Journal of Agricultural and Food Chemistry 60 (12): 3098–3104. doi: 10.1021/jf204715q.
Xie Z, Wu B, Shen G et al. (2017) Curcumin alleviates liver oxidative stress in type 1 diabetic rats. Mol Med Re-port. doi: 10.3892/mmr.2017.7911
Tapas A, Sakarkar D, Kakde R (2008) Flavonoids as Nutraceuticals: A Review. Tropical Journal of Pharma-ceutical Research 7 (3): 1089–1099. doi: 10.4314/tjpr.v7i3.14693.
Fukai T, Ushio-Fukai M (2011) Superoxide Dismutases: Role in Redox Signaling, Vascular Function, and Diseas-es. Antioxidants & Redox Signaling 15 (6): 1583–1606. doi: 10.1089/ars.2011.3999.
Lu M-C, Ji J-A, Jiang Z-Y, You Q-D (2016) The Keap1-Nrf2-ARE Pathway as a Potential Preventive and Thera-peutic Target: An Update: The Keap1-Nrf2-Are Pathway. Medicinal Research Reviews 36 (5): 924–963. doi: 10.1002/med.21396.
Velayutham R, Sankaradoss N, Ahamed KN (2012) Pro-tective effect of tannins from Ficus racemosa in hypercho-lesterolemia and diabetes induced vascular tissue damage in rats. Asian Pacific Journal of Tropical Medicine 5 (5): 367–373. doi: 10.1016/S1995-7645(12)60061-3.
Raish M, Ahmad A, Jan BL et al. (2016) Momordica charantia polysaccharides mitigate the progression of STZ induced diabetic nephropathy in rats. International Journal of Biological Macromolecules 91: 394–399. doi: 10.1016/j.ijbiomac.2016.05.090.
Reyazuddin M, Azmi S, Islam N, Rizvi A (2014) Oxida-tive stress and level of antioxidant enzymes in drug-naive schizophrenics. Indian Journal of Psychiatry 56 (4): 344. doi: 10.4103/0019-5545.146516.
Jiang T, Huang Z, Lin Y et al. (2010) The Protective Role of Nrf2 in Streptozotocin-Induced Diabetic Nephropathy. Diabetes 59 (4): 850–860. doi: 10.2337/db09-1342.
Tiwari BK, Pandey KB, Abidi AB, Rizvi SI (2013) Markers of Oxidative Stress during Diabetes Mellitus. Journal of Biomarkers 2013: 1–8. doi: 10.1155/2013/378790.
Bandeira S de M, Guedes G da S, Fonseca LJS da et al. (2012) Characterization of Blood Oxidative Stress in Type 2 Diabetes Mellitus Patients: Increase in Lipid Pe-roxidation and SOD Activity. Oxidative Medicine and Cellular Longevity 2012: 1–13. doi: 10.1155/2012/819310.
Stefanello N, Pereira LB, Schmatz R et al. (2015) Chlorogenic acid, caffeine and coffee reverse damages in liver, kidney and pancreas parameters of diabetic rats. Journal Diabetes Health 14.
Priska M, Peni N, Carvallo L (2019) Phytochemicals Screening and Antioxidant Effectiveness of Garlic (Alli-um sativum) from Timor Island. Biosaintifika: Journal of Biology & Biology Education 11 (1): 1–7. doi: 10.15294/biosaintifika.v11i1.17313.
Uluata S, McClements DJ, Decker EA (2015) How the Multiple Antioxidant Properties of Ascorbic Acid Affect Lipid Oxidation in Oil-in-Water Emulsions. Journal of Agricultural and Food Chemistry 63 (6): 1819–1824. doi: 10.1021/jf5053942.
Verma (2013) Phytochemical and Pharmacological Eval-uation of Selected Plants. American Journal of Biochem-istry and Biotechnology 9 (3): 291–299. doi: 10.3844/ajbbsp.2013.291.299.
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