Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Taguchi-optimized endoglucanase production by T. harzianum





Cellulase, Endoglucanase , Oil palm leaves, Solid-state fermentation, Taguchi orthogonal design, Trichoderma harzianum


The oil palm biomass produced by plantations and mills in Malaysia is the largest contributor to the nation’s agro-waste, with the oil palm leaves (OPL) topping the list. Nevertheless, the surplus of OPL might have applicability as the substrate for cultivating lignocellulolytic bio-degraders. Ipso facto, this study employed raw OPL as the carbon source for cultivating Trichoderma harzianum under solid-state fermentation (SSF). Optimization of the SSF process using the Taguchi orthogonal design to produce endoglucanase (CMCase) successfully established the optimal fermentation conditions as the following: 7.00 × 108 spore/g inoculum size, 50% moisture content, pH 12 Mandel’s medium, with 3-day incubation at 40°C. The crude enzyme cocktail exhibited the corresponding maximum activity of 417.49 U/g CMCase. The enzymatic activities were significantly affected by factors, moisture content, inoculum size, and initial pH (p-value < 0.05). In short, the high extracellular CMCase activity of the T. harzianum crude enzyme cocktail may prove valuable in accelerating the saccharification of cellulose for biofuel-and nanocellulose production.

Author Biographies

Hwee Li Teo, Miss

Ms. Hwee Li Teo was born in Batu Pahat, Johor, Malaysia in 1995. She obtained her Bachelor’s Degree in Chemistry in 2019. She was awarded the Academic Excellence Award by the Malaysian Institute of Chemistry (IKM) and the Dean’s Award by the Faculty of Science, Universiti Teknologi Malaysia (UTM) in 2019 upon graduation. Currently, she is pursuing her Ph.D at the Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia under the supervision of Associate Professor Dr. Roswanira Abdul Wahab. Her research focuses on the development of green protocol on biomass degradation and the production of oil palm leaves derived nanocellulose. 

Roswanira Abdul Wahab, Dept. of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia

Chemistry, Associate Professor


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