The Evaluation of Biochemical Parameters of Ethanolic Extract of Lepidium meyenii in Male Wistar Rats

Evaluation of Biochemical Parameters of Ethanolic Extract of Lepidium meyenii

Authors

  • Hamdalat Folake Muritala University of Illorin
  • Habeebat Oyewesi Department of Science Technology, Biochemistry unit, The Federal Polytechnic P.M.B 5351, Ado Ekiti, Ekiti State, Nigeria
  • Tobiloba ogunlana Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria
  • Loveth Ajakaiye Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria
  • Kehinde Aina Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria
  • Waleed Ibrahim Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria
  • Fiyinfoluwa Patricia Ogunyinka Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria
  • Halimat Aliyu Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria
  • Clement Olatunbosun Bewaji Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

DOI:

https://doi.org/10.11594/jtls.12.03.13

Abstract

The effects of ethanolic extract of Lepidium meyenii on some biochemical parameters were investigated. Forty male Wistar rats were grouped into five groups of eight animals each. Group 1 served as the control and received distilled water throughout the period of administration while groups 2, 3, 4 and 5 received 25, 50, 75 and 100 mg/kg body weight of ethanolic extract of L. meyenii respectively for seven and fourteen days. After seven days of administration, four animals from each of the groups were sacrificed and the rest were sacrificed after fourteen days, and biochemical alterations were monitored. The effects after the seven-day administration revealed that there was no significant (p > 0.05) difference in organ-body weight percentage, liver functional indices, kidney functional indices and the activity of some cytosolic and membrane bound enzymes studied but  following the fourteen-day administration, significant difference (p < 0.05) were observed with increase in organ-body weight percentage of selected organs, increase in serum activity of the enzymes: ALT, AST, ALP; and aberrant variation in the kidney functional indices studied at the 50, 75 and 100 mg/kg body weight showing selective toxicity of the extract. Inconclusive results were obtained in the analysis of the seminal parameters for both the seven- and fourteen-day administration while similar effects on hematological parameters were observed for both the seven- and fourteen-day administration. This study showed that the extract may be hepatotoxic and nephrotoxic when administered beyond seven days in male Wistar rats especially at 100 mg/kg body weight.

 

Keywords: Cytosolic, Haematology, Hepatotoxic, Membrane, Nephrotoxic

Author Biographies

Habeebat Oyewesi, Department of Science Technology, Biochemistry unit, The Federal Polytechnic P.M.B 5351, Ado Ekiti, Ekiti State, Nigeria

Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM
Johor, Malaysia

Department of Science Technology, Biochemistry unit, The Federal Polytechnic P.M.B 5351, Ado Ekiti, Ekiti
State, Nigeria

Tobiloba ogunlana, Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State,
Nigeria

Loveth Ajakaiye, Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State,
Nigeria

Kehinde Aina, Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State,
Nigeria

Waleed Ibrahim, Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State,
Nigeria

Fiyinfoluwa Patricia Ogunyinka, Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State,
Nigeria

Halimat Aliyu, Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State,
Nigeria

Clement Olatunbosun Bewaji, Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State, Nigeria

Department of Biochemistry, Faculty of Life Sciences, P.M.B. 1515, University of Ilorin, Ilorin, Kwara State,
Nigeria

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Published

2022-10-12

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