Effect of Different Plant Growth Regulators on Micropropagation of Some Pitaya Varieties : Micropropagation of Some Pitaya Varietie

Taner Bozkurt; Sezen Inan; Ijlal Dundar; Selda Kozak

doi:10.11594/jtls.12.02.04

Authors

Taner Bozkurt Tekfen Agricultural Research Production and Marketing Inc., Adana, Turkey
Sezen Inan Tekfen Agricultural Research Production and Marketing Inc., Adana, Turkey
Ijlal Dundar Tekfen Agricultural Research Production and Marketing Inc., Adana, Turkey
Selda Kozak Tekfen Agricultural Research Production and Marketing Inc., Adana, Turkey

DOI:

https://doi.org/10.11594/jtls.12.02.04

Keywords:

Pitaya, In vitro, Micropropagation, Royal Red, Seoul Kitchen, Siam Red, Physical Graffiti

Abstract

Pitaya belongs to the family Cactaceae and the genus Hylocereus. It is essential to
develop tissue culture protocols according to the appropriate variety to spread pitaya
commercial production and ensure healthy sapling production. This study aimed to
determine the best plant growth regulators (PGRs) in the micropropagation process,
and their effects on different pitaya cultivars were evaluated. Shoots of different pitaya
cultivars were cultured in Murashige and Skoog (MS) basal medium supplemented
with Indole-3-butyric (IBA), 6-benzyl amino purine (BAP), and gibberellic acid
(GA3). The highest micropropagation coefficient was determined in Physical Graffiti
variety cultured in MS medium supplemented with 2 mg/L BAP. The general evaluation based on the variety determined that the best micropropagation was in the Royal
Red variety. In in vitro rooting studies, the best rooting variety was Royal Red
(54.47%), followed by Siam Red (50.33%), Physical Graffiti (47.75%), and Seoul
Kitchen (44.82%). It was determined that the Royal Red variety is grown in R2 (MS
medium supplemented with 1 mg/l IBA) medium gave the best results in all criteria
of shoot length (4.28 cm), root length (6.45 cm), and root formation on the face
(74.48%). It was envisioned that these differences between the micropropagation,
growth, and rooting of the cultivars used in the studies resulted from the cultivar characteristics.
Keywords: Pitaya, In vitro, Micropropagation, Royal Red, Seoul Kitchen, Siam Red,
Physical Graffiti

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