In Silico Study of Eugenol and trans-Caryophyllene also Clove Oil Fumigant Toxicity on Tribolium castaneum

Authors

  • Silvi Ikawati Department of plant protection, Faculty of Agriculture, University of Brawijaya http://orcid.org/0000-0001-8731-2055
  • Toto Himawan Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia
  • Abdul Abadi Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia
  • Hagus Sarno Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia
  • Alvan Fajarudin Department of plant protection, Faculty of Agriculture, University of Brawijaya

DOI:

https://doi.org/10.11594/jtls.12.03.07

Keywords:

Docking, a botanical insecticide, Syzygium aromaticum, fumigant, eugenol, caryophyllene

Abstract

Alternative storage pest control that is more environmentally friendly than the use of synthetic chemical pesticides is to use botanical pesticides from plant essential oils, including clove (Syzygium aromaticum) which contains the main compounds eugenol and trans-caryophyllene. To study the various mechanisms of action of essential oils as botanical insecticides could use in silico approach through molecular docking. This study aims to predict the dominant binding mode(s) of a ligand with a protein of a known three-dimensional structure through docking. Then tested its fumigant activity on Tribolium castaneum. The docking results showed that the trans-caryophyllene and eugenol compounds had a more stable bond strength in the acetylcholinesterase enzyme T. castaneum than the control compound linalool. In addition, there is a synergy between eugenol and trans-caryophyllene when the two compounds interact with acetylcholinesterase. These results can be used as prediction material that trans-caryophyllene and eugenol have potential as protein acetylcholinesterase inhibitors of T. castaneum. After being tested in the laboratory, clove oil which contains two main compounds namely eugenol and trans-caryophylene has the potential to control T. castaneum with an LC50 value of 5,227 μL/L air. Keywords: Botanical insecticide, Caryophyllene, Docking, Eugenol, Fumigant,Syzygium aromaticum

Author Biographies

Silvi Ikawati, Department of plant protection, Faculty of Agriculture, University of Brawijaya

Department of plant protection, Faculty of Agriculture, University of Brawijaya

Toto Himawan, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia

Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145,Indonesia

Abdul Abadi, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia

Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145,Indonesia

Hagus Sarno, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia

Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang 65145,Indonesia

Alvan Fajarudin, Department of plant protection, Faculty of Agriculture, University of Brawijaya

Department of plant protection, Faculty of Agriculture, University of Brawijaya

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Published

2022-09-16