Prevalence of Antibiotic Resistance Bacteria in Aquaculture Sources in Johor, Malaysia
Prevalence of Antibiotic Resistance
DOI:
https://doi.org/10.11594/jtls.12.02.07Keywords:
Antibiotic resistance, multiple antibiotic resistance, aquaculture sources, MAR index value, 16S rRNA gene sequencingAbstract
The intensive use of antibiotics in aquaculture results in the proliferation of antibiotic.
In this study, antibiotic resistant bacteria from six different aquaculture sources (pond
of Fisheries Research Institute (FRI), and rivers of Kukup, Pulai, Pendas Laut, Sungai
Melayu and Kong Kong) were isolated. These isolates were tested for antibiotic resistance against seven antibiotics via the disc diffusion method. Finally, phenotypic
and genotypic identification via 16S rRNA sequencing and phylogenetic analysis
were carried out. The results show that 58 out of 118 bacterial isolates are resistant to
multiple antibiotics. The highest isolate resistance was observed towards rifampicin
(89.66%), followed by ampicillin (79.31%) and sulfafurazole (67.24%). The isolates
with multiple antibiotic resistant (MAR) index values with more than 20% were subjected to 16S rRNA gene sequencing. The majority of the bacterial strains exhibit
multiple antibiotic resistance, indicating that they were isolated from highly contaminated sources based on the tested water qualities profiles, which showed the high level
of turbidity and total dissolved solid (TDS) in most sampling sites with the high number of MAR bacteria obtained.
Keywords: Antibiotic resistance, Multiple antibiotic resistance, Aquaculture sources,
MAR index value, 16S rRNA gene sequencing
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