Environmental Conditions, Phytochemical Constituents, and Antibacterial Activities of Two Philippine Medicinal Vitaceae Species


  • Jeff Miranda Opena Cagayan State University - Gonzaga Campus, Cagayan, Philippines
  • Rachel Sotto




antibacterial property, Cayratia trifolia (Linn.) Domin, habitats and environment, plant secondary metabolites, Staphylococcus aureus, Tetrastigma harmandii Planch.


Plants are utilized for medicinal, therapeutic, nutritional, and industrial importance. This study investigated the phytochemical constituents and antibacterial activities of two medicinal Vitaceae species, namely: Cayratia trifolia and Tetrastigma harmandii. The growing environment (location, elevation, soil properties, and associated plants) of the species in northeastern Cagayan, Luzon, Philippines, were assessed and leaf samples were collected for phytochemical screening and antibacterial analysis. T. harmandii dominated the population found growing in various habitats such as hills, residential areas, near coastal areas and island shorelines, nipa plantations, agricultural and grasslands, near bodies of water, caves, and secondary growth forests while C. trifolia were only found growing in swamps, hills, and secondary growth forests. Both plants were found climbing in plant species like ipil-ipil (Leucaena leucocephala)and kakawate (Gliciridia sepium). T. harmandii populations were growing in soils with lower soil pH and higher nutrient content as compared to C. trifolia populations which grew in soils with higher pH and lower nutrients. Antioxidant properties were exhibited by the presence of secondary metabolites. Alkaloids, flavonoids, phenols, terpenoids, anthocyanins, tannins, and saponins were detected in C. trifolia ethanolic leaf extracts while phenols, terpenoids, tannins, and saponins were detected in T. harmandii. Both species showed inhibitory activity against Staphylococcus aureus. In addition, a slight activity against Klebsiella aerogenes was observed for T. harmandii. Thus, C. trifolia and T. harmandii also have antibacterial properties.

Author Biography

Jeff Miranda Opena, Cagayan State University - Gonzaga Campus, Cagayan, Philippines

College of Agriculture

Assistant Professor


Garden CA, Bennet HW (1956) The Toxic Plant of Western Australian Path. The West Australian News Paper.

Pelser PB, Nickrent DL, Barcelona JF (2016) Untangling a vine and its parasite: Host specificity of Philippine Rafflesia (Rafflesiaceae). Taxon 65(4): 739-758. doi: 10.12705/654.4.

Pranata S, Sulistijorini S, Chikmawati T. (2019) Ecology of Rafflesia arnoldii (Rafflesiaceae) in Pandam Gadang West Sumatra. Journal of Tropical Life Science 9(3): 243-252. doi: 10.11594/jtls.09.03.05.

Anita Jasuja ND, Mathur M (2019) Evaluation of Primary Metabolites and Antioxidant Potential Activity of Cayratia trifolia (Leaf and Stems). Journal of Drug Delivery and Therapeutics 9(4-A):367-372. doi: 10.22270/jddt.v9i4-A.3492.

Kumar D, Gupta J, Kumar S, Arya R, Kumar T, Gupta A (2012) Pharmacognostic evaluation of Cayratia trifolia (Linn.) leaf. Asian Pacific Journal of Tropical Biomedicine 2(1): 6-10. doi: 10.1016/S2221-1691(11)60180-9.

Arora J, Roat C, Goyal S, Ramawat KG (2009) High Stilbenes accumulation in root culture of Cayratia trifolia (L.) Domin grown in shake flask. Acta Physiologiae Plantarum 31: 1307-11. doi: 10.1007/s11738-009-0359-3.

Chellaperumal P, Sophia D, Arulraj C, Ragavendran P, Starlin T, Gopalakrishnan VK (2012) In vitro antioxidant activities and HPTLC analysis of ethanolic extract of Cayratia trifolia (L.). Asian Pacific Journal of Tropical Disease 952-956. doi: 10.1016/S2222-1808(12)60299-0.

Gupta J, Kumar D, Gupta A (2012) Evaluation of gastric anti–ulcer activity of methanolic extract of Cayratia trifolia in experimental animals. Asian Pacific Journal of Tropical Disease 2(2): 99-102. doi: 10.1016/S2222-1808(12)60024-3.

Kumar DG, Vattachanakkal MS, Rathi MA, Thirumoorthi L, Meenakshi P, Gopalakrishnan VK (2011) Hepatoprotective activity of Cayratia trifolia (L.) Domin against nitrobenzene induced hepatotoxicity. Latin American Journal of Pharmacy 30(3): 546-9.

Santoso D, Sudiana IK, Rahayu AS, Yunus M (2019) Anti-inflammatory effect of ethyl acetate fraction of galing plant extract (Cayratia trifolia) on male wistar rats induced by carrageenan. In Journal of Physics: Conference Series (Vol. 1146, No. 1, p. 012021). IOP Publishing. doi: 10.1088/1742-6596/1146/1/012021.

Arora J, Joshi A, Kanthaliya B, Khan F (2020) Effect of biotic elicitors on polyphenol production in Cayratia trifolia cell suspension cultures analyzed by HPLC. BioTechnologia 101(1): 35-43. doi: 10.5114/bta.2020.92926.

Batra S, Batra N, Nagori BP (2013) Preliminary phytochemical studies and evaluation of antidiabetic activity of roots of Cayratia trifolia (L.) Domin in alloxan induced diabetic albino rats. Journal of Applied Pharmaceutical Science 3(3), 97-100. doi: 10.7324/JAPS.2013.30319

Yusuf MI, Susanty S, Fawwaz M (2018) Antioxidant and antidiabetic potential of galing stem extract (Cayratia trifolia Domin). Pharmacognosy Journal 10(4): 686-689. doi: 10.5530/pj.2018.4.113.

Chungsamarnyart N, Sirinarumitr T, Chumsing W, Wajjawalku W (2007) In vitro study of antiviral activity of plant crude-extracts against the foot and mouth disease virus. Agriculture and Natural Resources 41(5): 97-103.

Rahmatullah M, Mollik MAH, Islam MK, Islam MR, Jahan FI, Khatun Z, Jahan R (2010) A survey of medicinal and functional food plants used by the folk medicinal practitioners of three villages in Sreepur Upazilla, Magura district, Bangladesh. American Eurasian Journal of Sustainable Agriculture 4(3): 363-373.

Quisumbing E (1951) Medicinal plants of the Philippines. Manila (Philippines): Bureau of Printing.

Brown, W.H. 1920. Minor products of Philippine forests Vol. 1, No.3. Department of Agriculture and Natural Resources, Bureau of Forestry Bulletin No. 22, Volume 3, Manila, Philippines. 329p. https://quod.lib.umich.edu/p/philamer/ADR0460.0001.003?rgn=main;view=fulltext. Accessed date: December 2020.

Motsara MR, Roy RN (2008) Guide to laboratory establishment for plant nutrient analysis. Rome, Italy. Food and Agriculture Organization of the United Nations.

Horneck DA, Sullivan DM, Owen JS, Hart JM (2011) Soil test interpretation guide. Oregon State University Extension Service, Corvallis, USA, 12 pp. https://ir.library.oregonstate.edu/downloads/00000020g. Accessed date: December 2020.

Pelser PB, Barcelona JF, Nickrent DL (2011) Vitaceae: Co’s Digital Flora of the Philippines. https://www.philippineplants.org/Families/Vitaceae.html?fbclid=IwAR0HTwRjTONNMRPGsbQvIuhGed0LMQBqMP5WZxt_qiLq3WTjtHMeMzIw7ok. Accessed date: November 2020.

Guevarra BQ (2005) A Guidebook to Plant Screening: Phytochemical and Biological. España, Manila, Philippines. UST Publishing House.

Edeoga HO, Okwu DE, Mbaebie BO (2005) Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4(7): 685-688. doi: 10.5897/AJB2005.000-3127.

Singh R, Singh S, Kumar S, Arora S (2007) Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food and Chemical Toxicology 45(7): 1216-1223. doi: 10.1016/j.fct.2007.01.002.

Shi J, Arunasalam K, Yeung D, Kakuda Y, Mittal G, Jiang Y (2004) Saponins from edible legumes: chemistry, processing, and health benefits. Journal of Medicinal Food 7(1): 67-78. doi: 10.1089/109662004322984734.

Cushnie TT, Lamb AJ (2005) Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents 26(5): 343-356. doi: 10.1016/j.ijantimicag.2005.09.002.

Popova MP, Chinou IB, Marekov IN, Bankova VS (2009) Terpenes with antimicrobial activity from Cretan propolis. Phytochemistry 70(10): 1262-1271. doi: 10.1016/j.phytochem.2009.07.025.

Akiyama H, Fujii K, Yamasaki O, Oono T, Iwatsuki K (2001) Antibacterial action of several tannins against Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 48(4): 487–491. doi: 10.1093/jac/48.4.487.

Avato P, Bucci R, Tava A, Vitali C, Rosato A, Bialy Z, Jurzysta M (2006) Antimicrobial activity of saponins from Medicago sp.: structureâ€activity relationship. Phytotherapy Research 20(6): 454-457. doi: 10.1002/ptr.1876.

Khan MI, Ahhmed A, Shin JH, Baek JS, Kim MY, Kim JD (2018) Green tea seed isolated saponins exerts antibacterial effects against various strains of gram positive and gram negative bacteria, a comprehensive study in vitro and in vivo. Evidence-Based Complementary and Alternative Medicine 2018: 1-12. doi: 10.1155/2018/3486106.

Lowy FD (1998) Staphylococcus aureus infections. New England Journal of Medicine 339(8): 520-532. doi: 10.1056/NEJM199808203390806.



2022-02-17 — Updated on 2022-02-17