Protein Profiling of Orthosiphon Stamineus Embryogenic Callus Using a Proteomic Approach

Protein Profiles of Orthosiphon stamineus Embryogenic Callus

Authors

  • Dayangku Dalilah Mamat Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.
  • Zaidah Rahmat Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.
  • Tsun-Thai Chai Department of Chemical Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia. Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia.
  • Fazilah Abd Manan Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.

DOI:

https://doi.org/10.11594/jtls.12.02.01

Keywords:

Embryogenic, Callus, Herb, Orthosiphon stamineus, Proteins

Abstract

Orthosiphon stamineus is one of the most prominent medicinal herbs widely grown
in Southeast Asia. Propagation of O. stamineus using tissue culture technique helps to
rapidly produce samples for research purposes, increase the production of secondary
metabolites and is considered as one of the strategies in plant genetic improvement.
Despite vast information on O. stamineus pharmacological properties, the protein profile of this species is currently underexplored. In the present study, proteins expressed
in embryogenic callus developed from O. stamineus leaves in Murashige and Skoog
medium supplemented with 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) were identified via Liquid chromatography- tandem mass
spectrometry (LC-MS/MS). The presence of embryogenic callus was confirmed with
microscope observation and Evan Blue assay. Using this proteomic approach, we
identified peptides that represent 22 proteins localized to different cell compartments
and involved in various biological and metabolic functions in plants. This finding is
useful to improve our understanding of protein functions, thus their future applications
could be further explored

 

Keywords: Callus, Embryogenic, Herb, Orthosiphon stamineus,Proteins

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Published

2022-05-17

Issue

Vol. 12 No. 2 (2022)

Section

Articles

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