Secondary Metabolite Ceiba pentandra Gaertn. as Biological Control to Canker Disease on Dragon Fruit
DOI:
https://doi.org/10.11594/jtls.11.03.10Keywords:
Canker, inhibitation, Neoscytalidium dimidiatum, severityAbstract
Canker diseases caused by Neoscytalidium dimidiatum are a serious threat for dragon fruit production. Comprehensive and environmentally friendly control efforts are needed to reduce yield losses due to this disease. Cottonwood (Ceiba pentandra) is one of the plants that contain complex phytochemicals that can control phyto-pathogens. This study aimed to examine the potential of secondary metabolites produced from the leaves and bark of the C. pentandara as botanical fungicides. Parts of dragon fruit that are attacked by canker are isolated on Potatoes Dextrose Agar media. Observation of conidia and fungal spores using a binocular microscope with a magnification of 100×. The hyphal form of the N. dimidiatum is rectangular and clear. As botanical fungicides, the leaves and the bark of the C. pentandara are dried for 14 days. The extract solution then analyzed for it’s secondary metabolites. The results of the qualitative analysis showed that each leaf extract and bark (+) compound of flavonoids, alkaloids, tannins, and saponins. The quantitative results of secondary metabolites detected 304.3 mg.kg-1, Flavonoids and Tannins of 1.6 mg.kg-1. Secondary metabolites obtained were tested on the fungus N. dimidiatum with inhibitory parameters in vitro and in vivo. This study followed a factorial completely randomized design consisting of concentration factors and types of extract parts. The data obtained were then analyzed by ANOVA and significant treatments were further tested by Duncan Multiple Range Test (DMRT) at α = 5%. The combination treatment with a concentration of 40 mg ml-1 is more effective in controlling canker in indicators of inhibitory, the extent of the attack, and intensity of disease severity.
References
Jumjunidang, Yanda RP, Rizka, Emilda D (2019) Identification and characterization of stem and fruit canker diseases on dragon fruit (Hylocereus spp.) in Indonesia. Journal of Horticulture 29(1): 103–110.
Mohd MH, Salleh B, Zakaria L (2013) Identification and molecular characterizations of Neoscytalidium dimidiatum causing stem canker of red-fleshed dragon fruit (Hylocereus polyrhizus) in Malaysia. Journal of Phytopatholo-gy 161: 841–849. doi: 10.1111/jph.12146.
Florez-Munoz SV, Gómez-Velásquez JC, Loaiza-DÃaz N et al. (2019) ITS rDNA gene analysis versus MALDI-TOF MS for identification of Neoscytalidium dimidiatum isolated from Onychomycosis and Dermatomycosis cases in Medellin (Colombia). Microorganism 7 (306): 1-10. doi:10.3390/microorganisms7090306.
Nouri MT, Laurence DP, Yaghmour MA et al. (2018) Neoscytalidium dimidiatum causing canker, shoot blight and fruit rot of almond in California. Plant Disease X(X): 1–10. doi: 10.1094/PDIS-12-17-1967-RE.
Al Raish SM, Saeed EE, Sham A et al. (2020) Molecular characterization and disease control of stem canker on royal poinciana (Delonix regia) caused by Neoscytalidium dimidiatum in the United Arab Emirates. International Journal of Molecular Science 21(1033): 1–17. doi:10.3990/ijms21031033.
Afrifa AB, Boateng AY (2012) Controlling dieback disease and its effect on the growth performance of a six-year Ceiba pentandra Stand. Journal of Agriculture & Social Sciences 8 (3): 106–108.
Ray JD, Burgess T, Lanoiselet V M (2010) First record of Neoscytalidium dimidiatum and N. novaehollandiae on Mangifera indica and N. dimidiatum on Ficus carica in Australia. Australasian Plant Disease Notes 5: 48–50. doi:10.1071/DN10018.
Jacobsen RE, Fantke P, Stefan T (2015) Analyzing half-lives for pesticide dissipation in plants. SAR and QSAR in Environmental Research 26(4): 325–342. doi: 10.1080/1062936X.2015.1034772.
Widiastuti A, Agustina W, Wibowo A, Sumardiyono C (2011) In vitro test pesticides efectiveness against some pathogens of important diseases in dragon fruit (Hylocerus spp). Jurnal Perlindungan Tanaman Indonesia 17 (2): 73-76.
Pathak R, Singh AK, Alkes T, Gehlot P (2018) Impact of climate change on host, pathogen, and plant diseases adaptation regime: a review. Bioscience Biotechnology Research Asia 15(3): 529-540. doi:10.13005/bbra/2658.
Yáñez-López R, Torres-Pacheco I, Guevara-González RG et al. (2012) The effect of climate change on plant diseases. African Journal of Biotechnology 11(10): 2417–2428. doi: 10.5897/AJB10.2442.
Hieu NT, Thu NTA, Linh DT et al. (2018) Effect of various degree of canopy pruning on plant growth, yield, and control of canker disease of dragon fruit crop: 2018; In-ernational Conference on Tropical Fruit Pests and Diseases.
Fullerton RA, Sutherland PA, Rebstock RS, Giang T (2015) The life cycle of dragon fruit canker caused by Neoscytalidium dimidiatum and implications for control. Dragon Fruit Regional Network Initiation Workshop. 71–80.
Hidayat NA, Sofian, Akhsan N (2018) Intensity of stem rot disease in dragon fruit (Hylocereus polyrhizus) at Samboja Regency. Agroteknologi Lembab 1(1): 53-60.
Peraza-ku SA, Cervantes-Uc JM, Morales BE, Calderon JAU (2020) Modification of ceiba pentandra cellulose for drug release applications. e-Polymare 20: 194–202. doi: 10.1515/epoly-2020-0025.
Kar S, Nehru J, Vishwavidyalaya et al. (2020) Cotton wood tree [Ceiba pentandra (L.) Gaertn]-an overview: 22 June 2020; Tikamgarh, College of Agriculture.
Syihabudin V, Sari BL, Utami NF, Apriliani NA (2018). Inhibitory activity of α -glucosidase of bark of Ceiba pentandra. Indonesian Journal of Pharmacology 29(4): 206–213. doi:10.14499/indonesianjpharm29iss4pp206.
Pratiwi RH (2014) Potential cottonwood (Ceiba pentandra) GAERTN. on traditional medicine service. E-Journal Widya Kesehatan dan Lingkungan 1 (1): 53-60.
Raji P, Samrot AV, Rohan DB et al. (2019) Extraction, characterization and invitro bioactivity evaluation of alkaloids, flavonoids, saponins and tannins of Cassia Alata, Thespesia Populnea, Euphorbia Hirta and Wrightia Tinctoria. Rayasan Journal Chemical 12 (1): 123–137. doi: 10.31788/RJC.2019.1214054.
Ajayi OE, Awala SI, Olalekan OT, Alabi OA (2012) Evaluation antimicrobial potency and phytochemical screening Persea americana leaf extract against selected bacterial and fungal isolates of clinical important. Microbiology Research Journal International 20 (1): 1-11. doi: 10.9734/MRJI/2017/24508.
Athipunyakom P, Seemadua S, Doungsaard C (2012) Dragon fruit (Pitaya) diseases in Thailand : 2012: Improving Pitaya Production and Marketing, Thailand.
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