Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction

Authors

  • Mohamad Rafi Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl Taman Kencana No. 3, Bogor 16128, Indonesia
  • Laela Wulansari Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl Taman Kencana No. 3, Bogor 16128, Indonesia
  • Dewi Anggraini Septaningsih Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl Taman Kencana No. 3, Bogor 16128, Indonesia
  • Tsania Firqin Purnomo Chemical Analyst Program, Vocational School, IPB University, Jl Kumbang No. 14 Kampus IPB Lodaya, Bogor 16128, Indonesia
  • Reza Auliatifani Chemical Analyst Program, Vocational School, IPB University, Jl Kumbang No. 14 Kampus IPB Lodaya, Bogor 16128, Indonesia
  • Khaydanur Khaydanur Chemical Analyst Program, Vocational School, IPB University, Jl Kumbang No. 14 Kampus IPB Lodaya, Bogor 16128, Indonesia
  • Auliya Ilmiawati Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia
  • Wina Yulianti Chemical Analyst Program, Vocational School, IPB University, Jl Kumbang No. 14 Kampus IPB Lodaya, Bogor 16128, Indonesia
  • Nunuk Kurniati Nengsih Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl Taman Kencana No. 3, Bogor 16128, Indonesia
  • Irma Herawati Suparto Primate Research Center, Institute of Research and Community Services, IPB University, Jl Lodaya II No. 5 Kampus IPB Lodaya, Bogor 16128, Indonesia
  • Wisnu Ananta Kusuma Department of Computer Science, Faculty of Mathematics and Natural Sciences, IPB University, Jl Meranti Kampus IPB Dramaga, Bogor 16680, Indonesia

DOI:

https://doi.org/10.11594/jtls.11.03.14

Keywords:

Antioxidant, FTIR spectra, Punica granatum, TLC fingerprint analysis, Principal component analysis

Abstract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.

Author Biography

  • Dewi Anggraini Septaningsih, Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl Taman Kencana No. 3, Bogor 16128, Indonesia

    Advance Research Laboratory, Institute of Research and Community Services, IPB University, Jl Palem Raya
    Kampus IPB Dramaga, Bogor 16880, Indonesia

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Published

2021-09-30

Issue

Vol. 11 No. 3 (2021)

Section

Articles

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