In vitro antagonistic study of maize root colonizing fungal isolates against Fusarium moniliforme causing ear rot disease of maize
Keywords:
Root colonizing plant growth promoting fungi, Maize rhizosphere, Fusarium moniliforme, Ear rot disease of maize, In vitro antagonism- Competition/Antibiosis.Abstract
In the present study, different root colonizing fungal isolates were isolated from the rhizospheric soil of maize growing areas of Uttar Dinajpur, West Bengal. All the isolates including test pathogen were identified from Indian Type Culture Collection, New Delhi. Plant growth promoting maize root colonizing fungal isolates- Penicillium pinophilum (ITC NO. 11,201.19), Trichoderma harzianum (ITC NO.11,203.19), Trichoderma asperellum (ITC NO. 11,209.19), Aspergillus niger (ITC NO. 11,204.19) and Penicillum purpurogenum (ITC NO. 11,207.19) exhibited antagonistic activities against F. moniliforme (ITC NO. 11,208.19) in vitro. Two antagonistic isolates of T. harzianum and eleven strains of T. asperellum showed antibiosis mechanism for antagonism against F. moniliforme with the range of Percent Inhibition of Radial Growth from 62.41% to 88.57%. Competitive mode of antagonism against test pathogen by the isolates of P. pinophilum, P. purpurogenum and six isolates of A. niger were found. Percent of inhibition of radial growth ranged from 57.14% to 91.42%.
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References
References
BenÃtez T, Rincon AM, Limon MC, Codon AC (2004) Biocontrol mechanisms of Trichoderma strains. Int Microbiol 7: 249-260.
Chaube HS, Singh US (1991) Plant disease management: principles and practices. CRC Press, Boca Raton, U.S.A.
Chet I, Inbar, J (1994) Biological control of fungal pathogens. Appl Biochem Biotech 48: 37-43.
Chet I (1987) Trichoderma- application, mode of action and potential as a biocontrol agent of soilborne plant pathogenic fungi. In: Chet I, ed, Innovative approaches to plant disease control. John Wiley and Sons, New York, USA. 137-160.
Gajera H, Domadiya R, Patel S, Kapopara M, Golakiya B (2013) Molecular mechanism of Trichoderma as bio-control agents against phytopathogen system – a review. Curr Res Microbiol Biotechnol 1:133-142.
Ghorbanpour M, Omidvari M, Abbaszadeh-Dahaji P, Omidvar R, Kariman K (2018) Mechanisms underlying the protective effects of beneficial fungi against plant diseases. Biol Control 117: 147–157.
Gwa VI, Nwankiti AO (2017) In vitro antagonistic potential of Trichoderma harzianum for biological control of Fusarium moniliforme isolated from Dioscorea rotundata tubers. Virol Mycol 6: 1-8.
Harman GE (2000) Myths and dogms of biocontrol. Plant Dis 84:377–393.
Kohl J, Kolnaar R, Ravensberg WJ (2019) Mode of action of microbial biological control agents against plant diseases: relevance beyond efficacy. Front Plant Sci 10: 1-19.
Krassilnikov NA (1950) Actinomycetes-antagonists and antibiotic substances (in Russian). Acad Sci USSR Mascow, Leningrad.
Manczinger L, Rakhely G, Vagvolgyi C, Szekeres A (2012) Genetic and biochemical diversity among Trichoderma isolates in soil samples from winter wheat fields of the Pannonian Plain. Acta Biol Szegediensis 56:141-149.
Mathre DE, Cook RJ, Callan NW (1999) From discovery to use: traversing the world of commercializing biocontrol agents for plant disease control. Plant Dis 83: 972–983.
Mendez A, Perez C, Montanez JC, Martinez G, Aguilar CN (2011) Red pigment production by Penicillium purpurogenum GH2 is influenced by pH and temperature. J Zhejiang Univ Sci B 12: 961–968.
Nelson PE (1992) Taxonomy and biology of Fusarium moniliforme. Mycopathologia 117: 29-36.
Pandey KK, Upadhya JP (2000) Microbial population from rhizosphere and non-rhzosphere soil of pegion pea. J Mycol Pl Pathol 30: 7-10.
Piotrowski M, Volmer JJ (2006) Cyanide metabolism in higher plants: cyanoalanine hydratase is a NIT4 homolog. Plant Mol Biol 61:111- 122.
Prakasapandian RT, Raja M, Kumar A, Sharma P (2016) Morphological and molecular characterization of Trichoderma asperellum strain Ta13. Indian Phytopathol 69: 298-303.
Sarrocco S, Guidi L, Fambrini S, Del’Innocenti E, Vannacci G (2009) Competition for cellulose exploitation between Rhizoctonia solani and two Trichoderma isolates in the decomposition of wheat straw. J Plant Pathol 91:331-338.
Sharma M, Gupta SK (2003) Ecofrindly methods for the management of root rot and web blight of french bean. J Mycol Pl Pathol 33: 345-361.
Singh F, Hooda L, Sindhan GS (2004) Biological control of tomato wilt caused by Fusrium oxsporium f. sp. lycopersici. J Mycol Pl Pathol 34: 568-570.
Singh US, Mishra DS, Rohilla R, Singh A, Vishwanath (2001) Induced resistance: present status and future prospects as disease management strategy. In: Opender Kaul GS, Dhaliwal SS, Marwaha, Arora J, eds, Biopesticides and pest management, Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum. Cam pus Book International, New Delhi.
Skidmore AM, Dickinson CH (1976) Colony interaction and hyphal interference between Septoria nodorum and phylloplane fungi. Trans Br Mycol Soc 66: 57-64.
Tarafdar JC, Bareja M, Panwar J (2003) Efficiency of some phosphatase producing soil fungi. Ind J Microbiol 43:27-32.
Zeilinger S, Galhaup C, Payer K, Woo SL, Mach RL, Fekete C, Lorito M, Kubicek CP (1999) Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet Biol 26:131-140.
Zulkifli NA, Zakaria L (2017) Morphological and molecular diversity of Aspergillus from corn grain used as livestock feed. Hayati J Biosci 1-9.
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