Utilization of Various Organic Wastes as Liquid Biofertilizer Carrier Agents towards Viability of Bacteria and Green Bean Growth

Authors

DOI:

https://doi.org/10.11594/jtls.12.01.01

Keywords:

vermiwash, molasses, compost wash, seaweed, pathogenicity test

Abstract

The problem in the production of biofertilizers is that raw materials are cheap, easy to get and apply. Another problem is determining the viability of the consortium microbes in a biofertilizer formulation. This study aims to determine the bacterial viability of various liquid media originating from organic waste as a liquid biofertilizer carrier. Three indigenous bacterial strains under consortium for phosphate soluble (Pantoea ananatis strain 53 (BC32)), non-symbiostic Nitrogen fixation (Bacillus licheniformis strain S45) and stabilizing soil aggregate (Pseudomonas plecoglossida
strain PR19) were added to liquid biofertilizer. The study evaluated 10 treatments using a randomized design with three replicates. The treatments are as follows: Peptone,
molasses, Compost wash from seaweed waste, Vermiwash, molasses + glycerol, compost wash from seaweed waste + glycerol, vermiwash + glycerol, molasses + PEG (PolyEthylene Glycols)1%, compost wash seaweed waste + PEG 1%, and Vermiwash + PEG 1%. This biofertilizer formulation (liquid) was kept for 16 weeks at optimum
pH 5.5. VP3 (Vermiwash made from vermicompost + PEG 1%) treatment showed the
best viability of bacterial strains during the 16-week storage period. The pathogenicity
test using green bean seeds Vima-1 showed that all liquid formulations of biological fertilizers with the three consortium bacterial isolates did not show signs of diseases and demonstrated better growth than the control treatment. Compared to other treatments, the best growth of bacterial strains was detected with MP2 (Molasses + glycerol) treatment. Formulations using vermiwash and PEG appear to maintain bacterial viability in the formulation effectively. However, the formulation of molasses and glycerol exerts a stimulating effect on sprouts growth.

References

Odlare M, Lindmark J, Ericsson A, Pell M (2015) Use of organic wastes in agriculture. Energy Procedia 75: 2472–2476. doi: 10.1016/j.egypro.2015.07.225

Jeni H, Aris I (2010) Viability and effectiveness of biofertlizer from several dried technique and period of storage. In Proceeding National Seminar Sains III, Biological Science: 2010; Bogor.

Utami SR, Ismunandar S (2009) Basic of soil science. Malang. Faculty of Agriculture, University of Brawijaya.

Sinha RK (2009) Earthworm vermicompost: A powerful crop nutrient over the conventional compost & protective soil conditioner agains the destructive chemical fertilizers for food safety and security, Am-Euras. Journal of Agriculture and Enviromental Science 5: 1-55.

Cocozza C, Parente A, Zaccone C, Mininni C, Santamaria P, Miano T (2011) Comparative management of offshore posidonia residues: composting vs. energy recovery. Waste Management (Oxford) 31: 78–84. doi: 10.1016/j.wasman.2010.08.016.

Fornes F, Sanchez PM, Guadiola JL (2002) Effect of a seaweed extract on the productivity of ‘de Nules’ clementinem danarin navelina orange. Botanica Marina 45: 486–489. doi: 10.1515/BOT.2002.051.

Prithiviraj B (2009) Seaweed extracts as biostimulants of plant growth dan development. Plant Growth Regulation 28: 386–399. doi: 10.1007/s00344-009-9103-x

Arfarita N, Lestari MW, Murwani I, Higuchi T (2019) Isolation of indigenous phosphate solubilizing bacteria from green bean rhizospheres. Journal of Degraded and Mining Lands Management 4(3): 845-851. doi: 10.15243/jdmlm.2017.043.845

Arfarita N, Muhibuddin A, Imai T (2019) Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna ertili for agricultural land treatment. Journal of Degraded and Mining Lands Management 6(2): 1617-1623. doi: 10.15243/jdmlm.2019.062.1617.

Pelczar MJ, Chan ECS (2005) Basic of microbiology. Translator: Hadioetomo. Jakarta. University of Indonesia.

Dastager SG, Deepa CK, Puneet SC, Nautiyal CS, Pandey A (2009) Isolation and characterization of plant growth-promoting strain Pantoea NII-186. From Western Ghat Forest Soil, India. Society for Applied Microbiology 49(1): 20-25. doi: 10.1111/j.1472-765X.2009.02616.x.

da Silva JF, Barbosa RR, de Souza AN, da Motta OV, Teixeira GN, Carvalho VS, de Souza ALSR, de Souza Filho GA (2015) Isolation of Pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion. Genetics and Molecular Research 14(4): 15301-15311. doi: 10.4238/2015.november.30.6.

Yang JH, Liu HX, Zhu GM, Pan YL (2007) Diversity analysis of antagonists from rice-associated bacteria and their application in biocontrol of rice diseases. J.Appl.Microbiol 104(1): 91-104. doi: 10.1111/j.1365-2672.2007.03534.x.

Amellal N, Burtin G, Bartoli L, Heulin T (1998) Colonization of wheat roots by an exopolysaccharide producing Pantoea agglomerans strain and its effect on rhizosphere soil aggregation. Appl. Environt. Microbiol 64(10): 3740-3747.

Bezzate S, Aymerich S, Chambert R, Czarnes S, Berge O, Heulin T (2000) Disruption of the Paenibacillus polymyxa levansucrase gene impairs ability to aggregate soil in the wheat rhizosphere. Environ Microbiol 2(3): 333–342. doi: 10.1046/j.1462-2920.2000.00114.x

Supriatin L (2008) Potency of antagonistic indigenous bacteria of peat land for controlling falling down disease (Sclerotium rolfsii) on soybean. PhD thesis. University of Brawijaya, Malang.

Arif M, Tariq M, Khan MU, Munir I (2008) Effect of seed priming on growth parameters of soybean. J. Bot 43(4): 2803-2812.

Simanungkalit RDM, Husen E, Saraswati (2008) Standart of biofertlizer and it’s controlling system. In: Organik ertilizer and Biofertilizer. Balai Besar Litbang Sumberdaya lahan, Bogor, 245-264.

USU (Universitas Sumatera Utara) (2014) Evaluation of bacterial content in the air using Media Plate Count Agar (PCA) based vertical height at the Departemen of mouth surgery at RSGMP FKG USU with Total Plate Count Methods (TPC). University of North Sumatera, Medan.

Giyanto A, Rustam S (2009) The study of culturing kitinolitik bacteria of Pseudomonas fluorescens and Bacillus sp. On organic waste and it’s formulation as biopesticide (BIOPesticide). Proceeding of Final Result Seminar, IPB, Bogor.

Martin AJ, Swarbrick, Cammarata N (1993) Physical fharmation: Basic of physical fharmacy on farmacetic science, Third edition. Transleter: Yoshita. Jakarta, UI Press. 1176-1182.

Morkunas I, Marzak J, Stachowiak, Stabiecki M (2005) Sucrose-induced lupine defense against Fusarium oxysporum: sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to Fusarium oxysporum. Plant Physiology and Biochemistry 43(4): 363-373. doi: 10.1016/j.plaphy.2005.02.011.

Park HG, Jeong MH, Ahn YS (2017a) Inoculation with Bacillus licheniformis MH48 to improve Camellia japonica seedling development in coastal lands. Turk. J. Agric 41: 381–388. doi: 10.3906/tar-1703-147.

Jae WS, Jun HW, Dong HK, Young SA (2019) The effect of Bacillus lichenisformis MH48 on control of foliar fungal diseases and growth promotion of Camellia oleifera seedlings in the coastal reclaimed land of Korea. MDPI 8(1): 6-19. doi: 10.3390/pathogens8010006.

Glick BR (2012) Plant growth-promoting bacteria: mechanisms and applications. Scientifica 1-15.doi: 10.6064/2012/963401.

Park HG, Lee YS, Kim KY, Park YS, Park KH, Han TH, Park CM, Ahn YS (2017b) Inoculation with Bacillus licheniformis MH48 promotes nutrient uptake in seedlings of the ornamental plant Camellia japonica grown in Korean reclaimed coastal lands. Hortic. Sci. Technol 35(1): 11–20. doi: 10.12972/kjhst.20170002.

Nishimori E, Kita T, Wakanbshi H (2000) Pseudomonas plecoglossicida the causative agent of bacterial haemorrhagic ascites of ayu (Plecoglossus altivelis). International Journal of System and Evolutionary Microbiology 50: 83-89. doi: 10.1099/00207713-50-1-83.

Silva HAS, Romeiro RSR, Macagnan D, Vieira BAH, Pereira MCB, Mounteer A (2015) Rhizobacterial induction of systemic resistance in tomato plants: non-specific protection and increase in enzyme activities. Biol Control 29: 288-295. doi: 10.1016/S1049-9644(03)00163-4.

Hugh Mc, D.J (2003) A Guide to Seaweed Industry. Food and Agriculture Oganization of the UN, 88.

Soeka YS, Rahayu SH, Setianingrum N, Naiola E (2011) The ability of Bacillus licheniformis in production of protease enzyme alkaline and thermophilic. Media Litbang Kesehatan 21(2): 89-95.

Wardhika CM, Suryanti, Joko T (2014) Eksploration of natural biological controlling bacteria Fusarium solani and Meloidogyne incognita in pepper. Journal of Indonesian Plant Protection 18: 90-95. doi: 10.22146/jpti.15608

Boricha H, Fulekar MH (2009) Pseudomonas plecoglossicida as a novel organism for the bioremediation of cypermethrin. Biology and Medicine 4(1): 1-10.

Rahmani S, Forozandeh M, Mosavp M, Rezaeej A (2006) Detection of bacteria by amplifying the 16S rRNA gene with universal primers and RFLP. Medical Journal of the Islamic Republic of Iran 19(4): 333-338.

Arfarita N, Hidayati N, Rosyidah A, Machfudz M, Higuchi T (2016) Exploration of indigenous soil bacteria producing-exopolysaccharides for stabilizing of aggregates land potential as biofertilizer. Journal of Degraded and Mining Lands Management 4(1): 697-702, 2016. doi: 10.15243/jdmlm.2016.041.697

Antastia W, Safni I, Siregar AZ (2019) Effectiveness test of several types of Plant Growth Promoting Rhizobacteria (PGPR) to control sprout disease (Athelia rolfsii (Curzi)) on Soybean (Glycine max (L.) Merril) plants. Jurnal Online Agroekoteknologi 7(2): 273-281.

Moghaddam FR, Aminpanah (2015) Green bean (Phaseolus vulgaris L.) growth and yield as affected by chemical phosphorus fertilizer and phosphate bio-fertilizer. IDESIA (Chile) 33(2).

Matloob AHA, Kamil SJ (2013) Biological control of bean root rot disease caused by Rhizoctonia solani under green house and field conditions. Agriculture & Biology Journal of North America 4: 512-519.

Cox DR (2009) Randomization in the Design of Experiments. International Statistical Review / Revue Internationale de Statistique 77(3): 415-429.

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2022-02-17 — Updated on 2022-02-17

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