Radiation sterilization of Honey and Honey-Alginate Wound Dressing from Stingless Bee (Tetragonula biroi) Collected from Sta. Maria, Laguna, Philippines

Authors

  • Davison Torres Baldos Department of Science and Technology - Philippine Nuclear Research Institute
  • Joseph Mallari Puno Department of Science and Technology - Philippine Nuclear Research Institute
  • Levelyn Mitos Mesa Tolentino Department of Science and Technology - Philippine Nuclear Research Institute
  • Djowel Recto Villanueva Montefalcon Department of Science and Technology - Philippine Nuclear Research Institute
  • Gilberto Tura Diano Department of Science and Technology - Philippine Nuclear Research Institute
  • Celia Oprenario Asaad Department of Science and Technology - Philippine Nuclear Research Institute

DOI:

https://doi.org/10.11594/jtls.11.02.11

Keywords:

alginate wound dressing, stingless bee honey, radiation sterilization

Abstract

This study was conducted to determine the effect of radiation sterilization on alginate wound dressing containing honey from the Philippine stingless bee, Tetragonula biroi. Our results show that a radiation dose of 30 kGy did not affect the antibacterial property of honey against Staphylococcus aureus. Electron-beam irradiation did not produce significant alterations in the physicochemical properties (pH, total soluble solids, and flavonoids); however, the total phenolics was significantly increased in honey with higher irradiation doses. Demonstrating that irradiation can be applied to honey with negligible physicochemical effects, honey was incorporated in alginate and exposed to a sterilization dose of 25 kGy using an electron beam facility.  Irradiation did not affect the physicochemical properties (pH, moisture content, gel fraction, moisture vapor transmission rate (MVTR), and fluid handling capacity) of the honey alginate wound dressing (HAWD). The perspectives for the potential use of irradiated HAWD as a natural product-based substitute for commercial wound care products may be considered.

Author Biographies

  • Davison Torres Baldos, Department of Science and Technology - Philippine Nuclear Research Institute
    Science Research Specialist I, Biomedical Research Section, Atomic Resarch Division
  • Joseph Mallari Puno, Department of Science and Technology - Philippine Nuclear Research Institute
    Science Research Analyst, Biomedical Research Section, Atomic Resarch Division
  • Levelyn Mitos Mesa Tolentino, Department of Science and Technology - Philippine Nuclear Research Institute
    Senior Science Research Specialist, Biomedical Research Section, Atomic Resarch Division
  • Djowel Recto Villanueva Montefalcon, Department of Science and Technology - Philippine Nuclear Research Institute
    Science Research Specialist I, Biomedical Research Section, Atomic Resarch Division
  • Gilberto Tura Diano, Department of Science and Technology - Philippine Nuclear Research Institute
    Science Research Specialist II, Biomedical Research Section, Atomic Resarch Division
  • Celia Oprenario Asaad, Department of Science and Technology - Philippine Nuclear Research Institute
    Supervising Science Research Specialist, Biomedical Research Section, Atomic Resarch Division

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Published

2021-06-09

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