Effect of alkaline protease produced from fish waste as substrate by Bacillus clausii on destaining of blood stained fabric
DOI:
https://doi.org/10.11594/jtls.11.01.08Keywords:
Alkaline protease, mass production, optimization, fish waste media, immobilization, Destaining.Abstract
Alkaline protease or peptidases are the largest group of enzymes in biological industry with variety of application in various industries to process of raw material, dehairing, diagnosis, extraction, food production and processing, destaining, etc., where the conditions prevails above neutral pH. Because of the wider applications the demand also increases for alkaline proteases, so required to produce in large scale cost effectively. An alkaline tolerant bacterial strain Bacillus clausii was isolated from fish waste and used for mass production of alkaline protease using fish waste homogenate as media. Preliminary study on optimization of conditions for the mass production carried out. The optimum temperature for protease production ranges between 25oC and 35oC and pH determined as 9. Along with fish waste homogenate, the mass production of extracellular alkaline protease from mobilized and immobilized cells of B. clausii carried out in production media, mixture of production media and fish waste homogenate and nutrient broth as standards. The recorded results showed that the maximum enzyme production obtained immobilized cells in nutrient broth media and followed by fish waste homogenate of 8900 U/ml and 8600 U/ml respectively. Purified enzyme yield was maximum obtained from production media 0.35 g/ml. Blood stained cloth treated with immobilized enzyme completely removed stain compared to treatment with non-immobilized enzyme and commercially used detergent. So the current study suggests the usage of microbial alkaline protease in house hold detergent to replace usage of chemicals and save the environment from chemical pollutants.
References
Singhal P, Nigam VK, Vidyarthi AS (2012) Studies on
production, characterization and applications of microbial alkaline proteases. International Journal of Advanced Biotechnology and Research 3 (3): 653 – 669.
Robinson PK (2015) Enzymes: principles and biotechnological applications. Essays in Biochemistry 59: 1–41.
doi: 10.1042/bse0590001.
Raveendran S, Parameswaran B, Ummalyma SB et al.
(2018) Applications of Microbial Enzymes in Food Industry. Food technology and biotechnology 56 (1): 16–
doi: 10.17113/ftb.56.01.18.5491
Razzaq A, Shamsi S, Ali A et al. (2019) Microbial proteases Applications. Frontiers in Bioengineering and Biotechnology 7: 110. doi: 10.3389/fbioe.2019.00110
Sasidharan A, Baiju KK, Mathew S (2013) Seafood processing waste management and its impact on local community in Cochin Corporation, India. International Journal of Environment and Waste Management 12 (4): 422-
doi: 10.1504/IJEWM.2013.056656
Zynudheen (2010) Utilisation of fishery waste in India.
Annual report of the Central Institute of Fisheries Technology. Kochi, CIFT Publication. 96 – 109.
Vazquez JA, Docasal SF, Miron J et al. (2006) Proteases
production by two Vibrio species on residuals marine
media. Journal of Industrial Microbiology and Biotechnology 33: 661– 668. doi: 10.1007/s10295-006-0096-1
Wang SL, Yeh PY (2006) Production of a surfactant- and
solvent-stable alkaliphilic protease by bioconversion of
shrimp shell wastes fermented by Bacillus subtilis
TKU007. Process Biochemistry 41: 1545–1552. doi:
1016/j.procbio.2006.02.018
Haddar A, Fakhfakh-Zouari N, Hmidet N et al. (2010)
Low- cost fermentation medium for alkaline protease
production by Bacillus mojavensis A21 using hulled
grain of wheat and Sardinella peptone. J Biosc Bioeng
: 288–294. doi: 10.1016/j.jbiosc.2010.03.015
Souissi N, Ellouz-Triki Y, Bougatef A et al. (2008) Preparation and use of media for protease-producing bacterial
strains based on by-products from Cuttlefish (Sepia officinalis) and wastewaters from marine-products processing factories. Microbiology Research 163: 473–480.
doi: 10.1016/j.micres.2006.07.013
Schallmey M, Singh A, Ward OP (2004) Developments
in the use of Bacillus species for industrial production. Canadian Journal of Microbiology 50: 1–17. doi:
1139/w03-076
Ramakrishna DPN, Reddy GN, Rajagopal SV (2010) Purification and properties of an extra Cellular Alkaline
protease produced by Bacillus Subtilis (MTTC N0-
. International Journal of Biochemistry and Biotechnology 6 (4): 493–504.
Wattiau P., Renard M, Ledent P et al. (2001) A PCR test
to identify Bacillus subtilis and closely related species
and its application to the monitoring of wastewater biotreatment. Appl Microbiol Biotechnol 56: 816–819. doi:
1007/s002530100691
Liu ZY, Wang Z, Zhang J (2008) An acidic protease
from grass carp intestine (Ctenopharyngodon idellus).
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 149: 83-90.
Geethanjali S (2016) Fish visceral protease – an alternative source for recovery of silver from waste x ray photographic films. Asian J Animal Sciences 11(2): 159-
doi: 10.15740/HAS/TAJAS/11.2/159-162.
Kageyama Y, Takaki Y, Shimamura S (2007) Intragenomic diversity of the V1 regions of 16S rRNA genes
in high-alkaline protease-producing Bacillus clausiis.
Extremophiles 11 (4): 597-603. doi: 10.1007/s00792-
-0074-1
Celandroni F, Vecchione A, Cara A et al. (2019) Identification of Bacillus species: Implication on the quality of
PS Kumari, R Reshma, 2021 / Effect of alkaline protease produced from fish waste
JTLS | Journal of Tropical Life Science 66 Volume 11 | Number 1 | January | 2021
probiotic formulations. PloSONE 14 (5): e0217021. doi:
1371/journal.pone.0217021
Kumaran E, Mahalakshmipriya A, Rajan S (2013) Effect
of fish waste based Bacillus protease in silver recovery
from waste X-ray films. International Journal of Current
Microbiology and Applied Sciences 2 (3): 49- 56.
Jabalia N, Mishra PC, Chaudhary N (2014) Applications,
Challenges and Future Prospects of Proteases: An Overview. Journal of Agroecology and Natural Resource
Management 1 (3): 179-183.
Geethanjali S, Subash A (2013) Optimization and Immobilization of Purified Labeo rohita Visceral Protease by
Entrapment Method. Enzyme Research Article
ID 874050. doi: 10.1155/2013/ 874050.
Kotwal SM, Shankar V (2009) Immobilized invertase.
Biotechnology Advances 27: 311-322.
Tanksale A, Chandra PM, Rao M, Deshpande V (2001)
Immobilization of alkaline protease from Conidiobolus
macrosporus for reuse and improved thermal stability.
Biotechnology Letters 23 (1): 51–54.
Kumar CG, Takagi H (1999) Microbial alkaline proteases from a bioindustrial viewpoint. Biotechnol Adv 17:
–594. doi: 10.1016/s0734-9750(99)00027-0.
Ellaiah P, Srinivasulu B, Adinarayana K (2002) A review on microbial alkaline proteases. J Sci Ind Res 61:
–704. doi: 123456789/26375
Guleria S, Walia A, Chauhan A, Shirkot CK (2016) Immobilization of Bacillus amyloliquefaciens SP1 and its
alkaline protease in various matrices for effective hydrolysis of casein. 3 Biotech 6: 208.
Annamalai N, Kumar A, Saravanakumar A et al. (2011)
Characterization of protease from Alcaligens faecalis
and its antibacterial activity on fish pathogens. Journal of
Environmental Biology 32: 781-786.
Anwar A, Saleemuddin M (1998) Alkaline proteases. A
Review. Bioresource Technology 6: 175-183. doi:
1016/S0960-8524(97)00182-X.
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