ISOLATION OF THERMOPHILIC LIPASE PRODUCING BACTERIUM FROM HOT SPRINGS AT THE EAST COAST OF PENINSULAR MALAYSIA

Authors

  • Tengku Haziyamin Tengku Abdul Hamid Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid, Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, MALAYSIA Tel: +609-570-4000 ext 5041 Fax: +609-571-6781 email: [email protected]
  • Nur Asimah Zainal Abidin Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid, Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, MALAYSIA Tel: +609-570-4000 ext 5041 Fax: +609-571-6781 email: [email protected]
  • Nurmusfirah Hasan Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid, Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, MALAYSIA Tel: +609-570-4000 ext 5041 Fax: +609-571-6781 email: [email protected]

DOI:

https://doi.org/10.11594/jtls.11.01.01

Keywords:

thermophilic organism, Geobacillus sp., thermophilic Bacillus, hot spring, thermostable lipases.

Abstract

Hot spring is a natural habitat for thermophilic bacteria and is the major source of thermostable enzymes useful in industrial application. In Malaysia, the search for thermophilic organisms have been focused on hot springs especially on the west coast of the peninsular. In this work, lipase producing thermophilic microorganisms were isolated from east coast hot springs in the states of Pahang and Terengganu. All of the 7 isolates showed the ability to grow at 45°C and formed lipolytic zones on tributyrin agar plates. However, only isolate B2B2 and S1B4 were able thrive at higher temperatures of up to 65°C. Bacillus and Geobacillus species was found to be the dominant bacterium isolated from these hot springs. From La hot spring, isolate L1 (MT 645486), LH2, (MT645483), LH3 (MT645484), LH4 (MT 645485) and LH5 (MT 645487) were all closely related to Bacillus sp. (94 to 97%). Meanwhile, from Bentong and Sungai Lembing hot springs, isolates B2B2 (MT668631) and S1B4 (MT668632) were closely related to Geobacillus kaustophilus (98.5%) and Geobacillus thermoleovorans (98%), respectively. Strains from Geobacillus sp. were able to thrive at higher temperature and their thermostable lipases have properties useful for biotechnological applications.

Author Biographies

  • Tengku Haziyamin Tengku Abdul Hamid, Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid, Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, MALAYSIA Tel: +609-570-4000 ext 5041 Fax: +609-571-6781 email: [email protected]
    Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid,
    Department of Biotechnology,
    Kulliyyah of Science,
    International Islamic University Malaysia,
    Kuantan Campus,
    Jalan Istana, Bandar Indera Mahkota,
    25200, Kuantan, Pahang,
    MALAYSIA
    Tel: +609-570-4000 ext 5041 
    Fax: +609-571-6781
    email: [email protected]
  • Nur Asimah Zainal Abidin, Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid, Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, MALAYSIA Tel: +609-570-4000 ext 5041 Fax: +609-571-6781 email: [email protected]
    Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid,
    Department of Biotechnology,
    Kulliyyah of Science,
    International Islamic University Malaysia,
    Kuantan Campus,
    Jalan Istana, Bandar Indera Mahkota,
    25200, Kuantan, Pahang,
    MALAYSIA
    Tel: +609-570-4000 ext 5041 
    Fax: +609-571-6781
    email: [email protected]
  • Nurmusfirah Hasan, Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid, Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia, Kuantan Campus, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, MALAYSIA Tel: +609-570-4000 ext 5041 Fax: +609-571-6781 email: [email protected]
    Assoc. Prof. Ts. Dr. Tengku Haziyamin Tengku Abdul Hamid,
    Department of Biotechnology,
    Kulliyyah of Science,
    International Islamic University Malaysia,
    Kuantan Campus,
    Jalan Istana, Bandar Indera Mahkota,
    25200, Kuantan, Pahang,
    MALAYSIA
    Tel: +609-570-4000 ext 5041 
    Fax: +609-571-6781
    email: [email protected]

References

Mehta R, Singhal P, Singh H, Damle D, and Sharma AK. (2016) Insight into thermophiles and their wide-spectrum applications. 3 Biotech,, 6(1): 81.

Salihu A and Alam M. (2015) Thermostable Lipases: an Overview of Production, Purification and Characterization. Biosciences Biotechnology Research Asia, 11: 1095-1107. DOI: 10.13005/bbra/1494.

Konwar BK and K. S, (2018), Lipase, in Lipase: an insdustrial enzyme through metagenomic. 2018, Apple Acedemic Press Inc.: Toronto. p. 12-13.

Sharma A, Sharma V, and Saxena J. (2016) A Review on Applications of Microbial Lipases. International Journal of Biotech Trends and Technology, 19: 1-5. DOI: 10.14445/22490183/IJBTT-V19P601.

Mazhar H, Abbas N, Ali S, Sohail A, Hussain Z, and Ali SS. (2017) Optimized production of lipase from Bacillus subtilis PCSIRNL-39. African Journal of Biotechnology, 16(19): 1106-1115.

BT M, HI AD, A J, A-L S, and C K. (2017) Isolation and Characterization of Thermophilic Bacteria from Jordanian Hot Springs: Bacillus licheniformis and Thermomonas hydrothermalis Isolates as Potential Producers of Thermostable Enzymes. International Journal of Microbiology, 2017: 6943952. DOI: 10.1155/2017/6943952.

Hassan B, Mohd N, Karl W, and Hariri AM. (2015) Geochemistry and geothermometry of non-volcanic hot springs in West Malaysia. Journal of Volcanology and Geothermal Research, 290: 12-22.

Samsudin AR, Hamzah U, Rahman RA, Siwar C, Jani MFM, and Othman R. (1997) Thermal springs of Malaysia and their potential development. Journal of Asian Earth Sciences, 15(2-3): 215-284.

Zuridah H, Norazwin N, Siti Aisyah M, Fakhruzzaman MNA, and Zeenathul NA. (2011) Identification of lipase producing thermophilic bacteria from Malaysian hot springs. African Journal of Microbiology Research 5(21): 3569-3573.

Chan CS, Chan K-G, Tay Y-L, Chua Y-H, and Goh KM. (2015) Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing. Frontiers in microbiology, 6: 177-177. DOI: 10.3389/fmicb.2015.00177.

Abdul Halim M, Rosli N, Dzulkifli F, Najimudin N, and Zarkasi K. (2020) Discovering the thermophiles microbial diversity of Malaysian hot spring in Ulu Slim, Perak. Malaysian Journal of Microbiology, 16: 184-192. DOI: 10.21161/mjm.190564.

Akanbi TO, Kamaruzaman AL, Bakar FA, Hamid N, Radu S, Manap MY, and Saari N. (2010) Highly thermostable extracellular lipase-producing Bacillus strain isolated from a Malaysian hotspring and identified using 16S rRNA gene sequencing. International Food Research Journal, 17: 45-53.

Yusuf MA and Hamid THT. (2013) Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus. Journal of Agrobiology, 30(1): 33-42.

Lemoine F, Correia D, Lefort V, Doppelt-Azeroual O, Mareuil F, Cohen-Boulakia S, and Gascuel O. (2019) NGPhylogeny.fr: new generation phylogenetic services for non-specialists. Nucleic Acids Research, 47(W1): W260-W265.

Zahoor S, Javed MM, and Babarl ME (2016). Brazilian Archives of Biology and Technology. 59, DOI: https://doi.org/10.1590/1678-4324-2016150662.

Gupta G, Srivastava S, Khare SK, and Prakash V. (2014) Extremophiles: an overview of microorganism from extreme environment. International Journal of Agriculture, Environment and Biotechnology, 7(2): 371-380.

Novik G, Savich V, and Meerovskaya O, (2019). Geobacillus Bacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy in Growing and Handling of Bacterial Cultures, M. Mishra, Editor IntechOpen: London, UK.

Kauldhar BS and Sooch BS. (2016) Tailoring nutritional and process variables for hyperproduction of catalase from a novel isolated bacterium Geobacillus sp. BSS-7. Microbial Cell Factories, 15(1): 7. DOI: 10.1186/s12934-016-0410-1.

Hriush Adhikari SG, Binod Khatri, Yuvraj, K.C. Polypasic analysis of two thermotolerant and exozymes producing Geobacillus species from hot spring of Nepal. Journal of Microbiology, Biotechnology and Food Sciences, 6(4): 1059-1064.

Rossi-Tamisier M, Benamar S, Raoult D, and Fournier PE. (2015) Cautionary tale of using 16S rRNA gene sequence similarity values in identification of human-associated bacterial species. International Journal of Systematic and Evolutionary Microbiology, 65(Pt 6): 1929-1934. DOI: https://doi.org/10.1099/ijs.0.000161.

Franco-Duarte R, Černáková L, Kadam S, Kaushik KS, Salehi B, Bevilacqua A, Corbo MR, Antolak H, Dybka-Stępień K, Leszczewicz M, Relison Tintino S, Alexandrino de Souza VC, Sharifi-Rad J, Coutinho HDM, Martins N, and Rodrigues CF. (2019) Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present. Microorganisms, 7(5): 130. DOI: 10.3390/microorganisms7050130.

Burgess SA, Flint SH, Lindsay D, Cox MP, and Biggs PJ. (2017) Insights into the Geobacillus stearothermophilus species based on phylogenomic principles. BMC Microbiology, 17(1): 140. DOI: 10.1186/s12866-017-1047-x.

DeFlaun MF, Fredrickson JK, Dong H, Pfiffner SM, Onstott TC, Balkwill DL, Streger SH, Stackebrandt E, Knoessen S, and van Heerden E. (2007) Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine. Systematic and Applied Microbiology, 30(2): 152-164. DOI: https://doi.org/10.1016/j.syapm.2006.04.003.

Kuisiene N, Raugalas J, Stuknyte M, and Chitavichius D. (2007) Identification of the genus Geobacillus using genus-specific primers, based on the 16S–23S rRNA gene internal transcribed spacer. FEMS Microbiology Letters, 277(2): 165-172. DOI: 10.1111/j.1574-6968.2007.00954.x.

Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA, Ivanova AE, Lysenko AM, Petrunyaka VV, Osipov GA, Belyaev SS, and Ivanov MV. (2001) Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th. International Journal of Systematic and Evolutionary Microbiology, 51(2): 433-446. DOI: https://doi.org/10.1099/00207713-51-2-433.

Norashirene MJ, Umi Sarah H, Siti Khairiyah MH, and Nurdiana S. (2013) Biochemical Characterization and 16S rDNA Sequencing of Lipolytic Thermophiles from Selayang Hot Spring, Malaysia,. IERI Procedia, 5: 258-264.

Chai YY, Kahar UM, Salleh MM, Illias RM, and Goh KM. (2012) Isolation and characterization of pullulan-degrading Anoxybacillus species isolated from Malaysian hot springs. Environmental Technology, 33(11): 1231-1238.

Sheikh Abdul Hamid N, Zen HB, Tein OB, Halifah YM, Saari N, and Bakar FA. (2003) Screening and identification of extracellular lipase-producing thermophilic bacteria from a Malaysian hot spring. World Journal of Microbiology and Biotechnology, 19(9): 961-968. DOI: 10.1023/B:WIBI.0000007330.84569.39.

Sakaff MKLM, Rahman AYA, Saito JA, Hou S, and Alam M. (2012) Complete genome sequence of the thermophilic bacterium Geobacillus thermoleovorans CCB_US3_UF5. Journal of Bacteriology: 1239.

Shariff FM, Rahman RNZRA, Basri M, and Salleh AB. (2011) A Newly Isolated Thermostable Lipase From Bacillus Sp. International Journal of Molecular Sciences, 12(5): 2917-2934.

Gandhi S, Salleh AB, Rahman RNZRA, Chor TL, and Oslan SN. (2015) Expression and Characterization of Geobacillus stearothermophilus SR74 Recombinant α-Amylase in Pichia pastoris. BioMed Research International, 2015: 529059. DOI: 10.1155/2015/529059.

Norazila Yusoff aNAI, (2012) (2012) Themostable alkaline serine protease from thermophilic bacillus species. International Research Journal of Biological Sciences, 2(2): 29-33.

Anuradha B, Darah I, Rashidah AR, Rashid A, and Azzahra F. (2012) Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra. Enzyme Research, 2012: 987523. DOI: 10.1155/2012/987523.

Ghazali SAM and Hamid THTA. (2015) New Lipase Producing b-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. Isolated from a Malaysian Hot Springs. Sains Malaysiana, 44(5): 701-706. DOI: 10.17576/jsm-2015-4405-09.

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Published

2021-02-03

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Vol. 11 No. 1 (2021)

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