Biosorption of Cadmium by fungi isolated from Bharalu river, Assam

Dipannita Deka, Pinky Bora, Nameirakpam Nirjanta Devi, Mayuri Chabukdhara

Abstract


Heavy metal pollution in the aquatic ecosystem poses a serious threat to the human health and aquatic biodiversity around. There is urgent need to remediate heavy metals contaminated wastewater through eco friendly ways. Fungi and other microorganisms have been reported to have promising potential to remove heavy metals from wastewater through biosorption. Thus the present work aimed to isolate tolerant fungal species from different sites of a polluted river Bharalu in Assam. After preliminary screening, out of a total of 15 fungal isolates obtained, 4 isolates Aspergillus niger, Aspergillus flavus, Aspergillus fumigates and Colletotrichum gloeosporioides were selected for further study. Tolerance limit of the isolates for Cadmium (Cd) was carried out at concentrations ranging from 100 ppm to 1000 ppm. Results showed that the maximum tolerance was observed in Colletotrichum gloeosporioides (approx. 8.8 mm), followed by Aspergillus flavus (approx. 5.8 mm) at 400ppm. The biosorption efficiency at 400ppm revealed that the maximum Cd concentration was absorbed by fungal biomass of Colletotrichum gloeosporioides (12.83 mg/g) and the minimum by Aspergillus niger (3.91 mg/g). Molecular identification was carried out followed by PCR amplification of the fungal biomass showing highest absorption. The sequence obtained was submitted in the GenBank using BANkIt with accession number-MN714368 which showed high similarities with Colletotrichum gloeosporioides. This results indicated that fungus are promising and potential sources for metal biosorption and detoxification.


Keywords


Bioremediatin;heavy metals;fungi

Full Text:

PDF

References


Wu. R.S.S., Lau T.C., Fung W.K.M., Koa P.H., Leung K.M.Y (2007). An artificial mussel for monitoring heavy metals in a marine environments. Environ. Pollut. 145, 104–110.

Kibria G., Lau T.C, Wu. R (2012) Innovative ‘Artificial Mussels’ technology for assessing

spatial and temporal distribution of metals in Goulburn-Murray catchments waterways, Victoria, Australia: Effects of climate variability (dry vs. wet years). Environ. Int. 50C, 38–46.

Wang G., Wang L, Ma F, You Y, Wang Y, Yang D (2019) Integration of earthworms and arbuscular mycorrhizal fungi into phytoremediation of cadmium-contaminated soil by Solanum nigrum L. Journal of Hazardous Materials. https://doi.org/10.1016/j.jhazmat.2019.121873.

Fazli M.M, Soleimani N, Mehrasbi M, Darabian S, Mohammadi J, Ramazani A ( 2015) Highly cadmium tolerant fungi: their tolerance and removal potential. Journal of Environmental Health

Science & Engineering 13:19. Doi 10.1186/s40201-015-0176-0.

Roy S, Kalita J C (2011) Identification of Estrogenic Heavy metals in Water Bodies Around Guwahati City, Assam, India. International Journal of ChemTech Research 3(2) 699-702. ISSN : 0974-4290 .

Bai SR, Abrahim TE (2003) Studies on chromium (VI) Adsorption–desorption using immobilized fungal biomass. Bioresour Technol 87:17–26

Joshi P.K, Swarup Anand, Maheshwari Sonu, Kumar Raman, Singh Namita (2011). Bioremediation of Heavy Metals in Liquid Media Through Fungi Isolated from Contaminated Sources. Indian J Microbiol 51(4):482–487. Doi 10.1007/s12088-011-0110-9

Manguilimotan L.C and Bitacura J.G (2018) Biosorption of Cadmium by Filamentous Fungi Isolated from Coastal Water and Sediments. Journal of Toxicology6. https://doi.org/10.1155/2018/7170510

Kornerup, Andreas and Wansher, Johan H (1978).Methuen Handbook of Colour, Eyre Methuen 3:252 .

Silva D.M, Batista L.R, Rezende E.F, Fungaro M.H.P, Sartori D, Alves E (2011) Identification Of Fungi Of The Genus Aspergillus Section Nigri Using Polyphasic Taxonomy. Brazilian Journal of Microbiology 42: 761-773.

Shamly V, Kali A, Srirangaraj S, Umadevi S (2014) Comparison of Microscopic Morphology of Fungi Using Lactophenol Cotton Blue (LPCB), Iodine Glycerol and Congo Red Formaldehyde Staining. Journal of Clinical and Diagnostic Research 8(7). DOI: 10.7860/JCDR/2014/8521.4535.

Oladipo O.G, Awotoye O.O , Olayinka A, Bezuidenhout C.C, Maboeta M.S (2018) Heavy metal tolerance traits of filamentous fungi isolated from gold and gemstone mining sites. Brazilian journal of microbiology 49. 29–37. ht t p : / /www.bjmicrobiol.com.br/ .

EPA, U., (1996) Method 3050B: acid digestion of sediments, sludges, and soils. Environ. Prot. Agency 2, 3e5.

Chabukdhara M, Nema AK (2012) Assessment of heavy metal contamination in Hindon River sediments: A chemometric and geochemical approach. Chemosphere 87 : 945cont.

Doyle J J, Doyle J L (1987) A rapid DNA isolation procedure for small quatities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.

Tripathy S.K, Maharana M, Ithape D.M, Lenka D, Mishra D, Prusti A, Swain D, Mohanty M.R and K.R. Reshmi Raj (2017) Exploring Rapid and Efficient Protocol for Isolation of Fungal DNA. Int.J.Curr.Microbiol.App.Sci. 6(3): 951-960. http://www.ijcmas.com.

Umesha S, Manukumar H.M, Raghava S (2016) A rapid method for isolation of genomic DNA from food-borne fungal pathogens 6:123. DOI 10.1007/s13205-016-0436-4.

White T J, Bruns T, Lee S, Taylor J L (1990) Amplification and Direct Sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols:a guide to methods and applications 18: 315-322.

Ezzouhri L, Castro E, Moya M, Espinola F and Lairini K (2009) Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco. African Journal of Microbiology Research 3(2):035-048 . http://www.academicjournals.org/ajmr .

Rodriguez I.A , Cardenas-Gonzalez J.F, Perez Adriana S. R, Oviedo J.T and Martinez-Juarez Victor M (2018) Bioremoval of Different Heavy Metals by the Resistant Fungal Strain Aspergillus niger. Bioinorganic Chemistry and Applications 7. https://doi.org/10.1155/2018/3457196 .

Dwivedi S, Mishra A, Saini D (2012) Removal of Heavy Metals in Liquid Media through Fungi Isolated from Waste Water. International Journal of Science and Research (IJSR) 1( 3) .

Sharma, G.D, Hijri Mohamed (2014) Isolation and Screening of Some Filamentous Fungi with Various Trace Metals. Microbiology 4(2). ISSN - 2249-555X.

Veglio F, Beolcmi F (1997) Removal of metals by biosorption: a review. J Hydrometall 74:301–316.

Joo Jin Hoo and Hussein Khalid A (2012) Heavy Metal Tolerance of Fungi Isolated from Contaminated Soil. Korean J. Soil Sci. Fert. 45(4):565-571. http://dx.doi.org/10.7745/KJSSF.




DOI: http://dx.doi.org/10.11594/jtls.11.03.04

Copyright (c) 2021 Journal of Tropical Life Science