A Case Study of the Phenotypic Variations in Barilius Bendelisis (Hamilton) from a Perennial Stream and a Fish Pond of Garhwal Himalayan Region of Uttarakhand, India

Pranav Singh, Shivani Nautiyal, Navneet Swami, Jitendra Singh Rana, Manju Prakash Gusain, Om Prakash Gusain


A comparative study on the morphometric and meristic variables of Barilius bendelisis (Ham.) from two different water bodies, i.e., Khanda Gad, a perennial spring fed stream and fish pond in the Garhwal Himalayan region of Uttarakhand was made during the present study. A total of 100 specimens were analysed for 26 different morphometric variables and 14 meristic counts. The majority of morphometric variables showed linear relationship when expressed in relation to total length and head length, whereas meristic counts remained constant with increasing body length. Standard length was found to be the highly correlated character in samples from both sites. Principal Component Analysis of 10 significant morphometric variables yielded three components accounting for 73.38% of the total variation. Principal Component Analysis of 3 meristic variables yielded single component accounting for 62.3% of total variation. Discriminant Function Analysis for morphometric and meristic variables showed that 98% and 83% of individuals were allocated into their original populations respectively. The cluster analysis for morphometric characters showed of fish populations from both sites formed two major clades, thus significantly differentiating the two stocks of fish population.


Discriminant Function Analysis; Morphometric Measurement; Phenotypic characters; Principal Component Analysis

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Murta AG (2000) Morphological variation of horse mackerel (Trachurus trachurus) in the Iberian and North African Atlantic: implications for stock identification. ICES Journal of Marine Science 57 (4): 1240–1248. doi: 10.1006/jmsc.2000.0810.

Pinheiro A, Teixeira CM, Rego AL, Marques JF, Cabral HN (2005) Genetic and morphological variation of Solea lascaris (Risso, 1810) along the Portuguese coast. Fisheries Research 73 (1–2): 67–78. doi: 10.1016/j.fishres.2005.01.004.

Buj I, Podnar M, Mrakovčić M, Ćaleta M, Mustafić P, Zanella D, Marčić Z (2008) Morphological and genetic diversity of Sabanejewia balcanica in Croatia. Folia Zoologica 57 (1–2): 100–10.

Torres R, Gonzalez P, Pena S (2010) Anatomical, histological and ultraestructural description of the gills and liver of the Tilapia (Oreochromis niloticus). International Journal of Morphology 28 (3): 703–712. doi: 10.4067/S0717-95022010000300008.

Jayaram KC (2002) Fundamentals of fish taxonomy. Narendra Publishing House, Delhi.

Elliott NG, Haskard K, Koslow JA (1995) Morphometric analysis of orange roughy (Hoplostethus atlanticus) off the continental slope of southern Australia. Journal of Fish Biology 46 (2): 202–220. doi: 10.1111/j.1095-8649.1995.tb05962.x.

Johal MS, Kaur A (2005) Morphometry and Barilius bendelisis (Ham., 1822) from hillstreams of Himachal Pradesh, India. In Proceedings of the national seminar on New trends in fishery development in India. Punjab University, Chandigarh. 23–28.

Johal MS, Tandon KK, Sandhu GS (1994) Morphometry of Tor putitora (Mahseer) in lacustrine waters Gobind Sagar reservoir. In: Nautiyal P, eds. Mahseer the game fish. Jagdamba Prakashan Publishers, Dehradun, Rachna, Srinagar, Garhwal, B. 67–85.

Hard JJ, Berejikian BA, Tezak EP, Schroder SL, Knudsen CM, Parker LT (2000) Evidence for morphometric differentiation of wild and captively reared adult Coho salmon: a geometric analysis. Environmental Biology of Fishes 58 (1): 61–73. doi: 10.1023/A:1007646332666.

Kim SK, Yoon SC, Youn SH, Park SU, Corpus LS, Jang IK (2013) Morphometric changes in the cultured starry flounder, Platichthys stellatus, in open marine ranching areas. Journal of Environmental Biology 34: 197–204.

Patiyal RS, Mir JI, Sharma RC, Chandra S, Mahanta PC (2014) Pattern of meristic and morphometric variations between wild and captive stocks of endangered Tor putitora (Hamilton, 1822) using multivariate statistical analysis methods. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 84 (1): 123–129. doi: 10.1007/s40011-013-0206-6.

Hanson KC, Hasler CT, Suski, CD, Cooke SJ (2007) Morphological correlates of swimming activity in wild largemouth bass (Micropterus salmoides) in their natural environment. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology 148 (4): 913–920. doi: 10.1016/j.cbpa.2007.09.013.

Basaran F, Ozbilgin H, Ozbilgin YD (2007) Comparison of the swimming performance of farmed and wild gilthead sea bream, Sparus aurata. Aquaculture Research 38 (5): 452–456. doi: 10.1111/j.1365-2109.2007.01670.x.

Rogdakis YG, Koukou KK, Ramfos A, Dimitriou E, Katselis GN (2011) Comparative morphology of wild, farmed and hatchery–released gilthead sea bream (Sparus aurata) in western Greece. International Journal of Fisheries and Aquaculture 3 (1): 1–9.

Ahmad MF, Iqbal MJ, Chandra S (2014) Detection of morphometric differentiation in Sattar snowtrout, Schizothorax curvifrons (Cypriniformes: Cyprinidae) from Kashmir Himalaya using a truss network system. Revista de Biologia Tropical 62 (1): 132–141.

Turan C (1999) A note on the examination of morphometric differentiation among fish populations: the truss system. Turkish Journal of Zoology 23 (3): 259–264.

Swain DP, Foote CJ (1999) Stocks and chameleons: the use of phenotypic variation in stock identification. Fisheries Research 43 (1–3): 113–128. doi: 10.1016/S0165-7836(99)00069-7.

Keast A (1978) Trophic and spatial interrelationships in the fish species of an Ontario temperate lake. Environmental Biology of Fishes 3 (1): 7–31. doi: 10.1007/BF00006306.

DOI: http://dx.doi.org/10.11594/jtls.10.03.02

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