Application of Atmospheric Pressure Argon Plasma Jet (APAPJ) in Biomedical Science and Engineering

Jyoti Prakash Gurung, Deepak Prasad Subedi, Rajendra Shrestha, Bhupal Govinda Shrestha

Abstract


In this paper, we demonstrated the application of atmospheric pressure argon plasma jet (APAPJ) in biomedical science such as cancer therapy and biomedical engineering such as surface modification of polystyrene Petri dishes for animal cell culture. We observed that APAPJ, when exposed to breast cancer cell line (MDA-MB-231) for 60 seconds at a distance of ~ 2.5 cm, reduced the cancer cells by 57%. This reduction in cancer cell concentration demonstrated the cancer-killing property of APAPJ. Further optimization of APAPJ's various operational parameters and in-depth biochemical study is required to figure out the mechanism responsible for killing cancer cells. Also, we investigated cell adhesion and proliferation due to APAPJ treatment by Crystal Violet (CV) staining and MTT Assay. We observed that APAPJ treatment of polystyrene Petri dishes for 60 - 240 s at a distance of ~ 3.5 cm exhibited enhancement in adhesion of breast cancer cell line (MDA-MB-231). We also demonstrated the increase in hydrophilicity by a reduction in contact angle to 30.3°±3 after 240 seconds of APAPJ treatment of polystyrene samples. The hydrophilicity of the substrate has proven to enhance cell adhesion. Consequently, APAPJ could be employed as a simple, inexpensive and environmentally friendly method for surface modification of polystyrene plates for animal cell culture.


Keywords


APAPJ; breast cancer cells; MTT assay; contact angle

Full Text:

PDF

References


Shrestha R, Subedi DP, Adhikari S et al. (2017) Exper-imental study of atmospheric pressure argon plasma jet-induced strand breakage in large DNA molecules. Plas-ma Medicine 7 (1): 65 – 76. doi: 10.1615/PlasmaMed.2017019986.

Shrestha R,Gurung JP, Subedi DP et al. (2016) Genera-tion, characterization and application of atmospheric pressure plasma jet. Sains Malaysiana 45 (11): 1689-1696.

Dubuc A, Monsarrat P, Virard F et al. (2018) Use of cold-atmospheric plasma in oncology: a concise system-atic review. Therapeutic Advances in Medical Oncology 10: 1758835918786475. doi: 10.1177/17588359187864

Tanaka H, Mizuno M, Ishikawa K et al. (2018) Molecu-lar mechanisms of non-thermal plasma-induced effects in cancer cells. Biological Chemistry 400 (1): 87–91. doi: 10.1515/hsz-2018-0199.

Lerman MJ, Lembong J, Muramoto S et al. (2018) The Evolution of Polystyrene as a Cell Culture Material. Tis

sue Engineering Part B: Reviews 24 (5): 359–372. doi: 10.1089/ten.teb.2018.0056.

Bilek MMM (2014) Biofunctionalization of surfaces by energetic ion implantation: Review of progress on applications in implantable biomedical devices and antibody microarrays. Applied Surface Science 310: 3 – 10. doi:10.1016/j.apsusc.2014.03.097.

Yonson S, Coulombe S, Léveillé V, Leask RL (2006) Cell treatment and surface functionalization using a miniature atmospheric pressure glow discharge plasma torch. Journal of Physics D: Applied Physics 39 (16): 3508 – 3513. doi: 10.1088/0022-3727/39/16/S08

Napoli C, Marcotrigiano V, Montagna MT (2012) Air sampling procedures to evaluate microbial contamination: a comparison between active and passive methods in operating theatres. BMC Public Health 12: 594. doi: 10.1186/1471-2458-12-594

Srinivasan S, McKinley GH, Cohen RE (2011) Assessing the Accuracy of Contact Angle Measurements for Sessile Drops on Liquid-Repellent Surfaces. Lang-muir 27 (22): 13582 – 13589. doi: 10.1021/la2031208.

Arima Y, Iwata H (2007) Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayers. Journal of Materials Chemistry 17 (38): 4079 – 4087. doi: 10.1039/B708099A.




DOI: http://dx.doi.org/10.11594/jtls.10.02.07

Copyright (c) 2020 Journal of Tropical Life Science