Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume)
DOI:
https://doi.org/10.11594/jtls.10.02.02Keywords:
Amorphophallus muelleri, pathogen bacteria, tuber rot, porangAbstract
Tuber rot has been reported as one of the limiting factors to porang (Amorphophallus muelleri Blume) tuber production. Bacterial infection is one of the main causes of tuber rot during storage. In this study, a total of seven bacterial isolates obtained from porang tubers were tested for their potency to cause rotting on porang tubers. Porang tubers were immersed in bacterial suspension and incubated for 14 days. The physical properties of the tubers such as texture and color, and the chemical properties such as reducing sugar and ethanol content were determined at the end of incubation period. All infected tubers were soft, slimy and darker. Two isolates, T4 and T9A, had the highest potency to cause tuber rot indicated by the significantly higher reducing sugar content (14.38 and 15.32 mg/ml) and higher ethanol content (32.17 and 35.05%) compared to control tubers which were not infected with bacteria. Both isolates were identified as Bacillus altitudinis and Pseudomonas stutzeri, respectively, based on 16S rDNA sequence analysis.
References
Mekkerdchoo O, Borompichaichartkul C, Perrigo AL, Srzednicki G, Prakitchaiwattana C, Antonelli A (2016) Tracing the evolution and economic potential of konjac glucomannan in Amorphophallus species (Araceae) using molecular phylogeny and RAPD markers. Phytotaxa 28 (2): 81 – 106. doi : 10.11646/phytotaxa.282.2.1.
Santosa E, Susila AD, Lontoh AP, Mine Y, Sugiyama N (2016) NPK levels and application methods on productivity of Amorphophallus muelleri Blume in intercropping system. Jurnal Jamu Indonesia 1 (2): 1 – 8. doi: 10.29244/jji.v1i2.12.
Dwiyono K, Sunarti TC, Suparno O, Haditjaroko L (2014) Penanganan pascapanen umbi iles-iles (Amorphophallus muelleri Blume) studi kasus di Madiun, Jawa Timur. Jurnal Teknologi Industri Pertanian 24 (3): 179 – 188.
Nourian F, Kushalappa AC, Ramaswamy HS (2002) Physical, physiological and chemical changes in potato as influenced by Erwinia carotovora infection. Journal of Food Processing and Preservation 26 (5): 339 – 359. doi: 10.1111/j.1745-4549.2002.tb00489.x.
Wu J, Yang C, Jiao Z, Hu Z, Zili D, Qiu Z (2015). Genetic relationships of soft rot bacteria isolated from konjac in China by Amplified Fragment Length Polymorphism (AFLP) and 16S rDNA gene sequences. Agricultural Sciences 6 (7): 717 – 723. doi: 10.4236/as.2015.67069.
Feia HE, Duan JL, Luo BF, Zhang ZL, Liu LP, Zhang XM (2013) Pathogenic bacteria biodiversity of Amorphophallus infected with soft rot disease in Langao, Shaanxi. Journal of Northwest Agriculture University 41 (7): 91 – 98.
Pérombelon MCM (2002) Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathology 51 (1): 1 – 12. doi: 10.1046/j.0032-0862.2001.Shorttitle.doc.x.
Waterer DR, Pritchard MK (2009) Production of volatile metabolites in potatoes infected by Erwinia carotovora var. carotovora and E. carotovora var. atroseptica. Canadian Journal of Plant Pathology 7 (1): 47 – 51. https://doi.org/10.1080/07060668509501513.
Keusalya H, Baharuddin, Zakaria B, Syaiful SA (2015) Isolation and physiological characterization of PGPR from potato plant rhizosphere in medium land of Buru Island. Procedia Food Sciences 3: 190 – 199. https://doi.org/10.1016/j.profoo.2015.01.021.
Arfani N, Azrianingasih R, Suharjono (2017) Isolation and identification of antagonistic bacterium against pathogens of bacterial tuber rot of Amorpophallus muelleri. Journal of Experimental Life Science 8 (3): 165 – 172. http://dx.doi.org/10.21776/ub.jels.2018.008.03.06.
Sakaroni R, Suharjono, Azrianingsih R (2019) Identification of potential pathogen fungi which cause rotten on porang (Amorphophallus muelleri Blume) tubers. AIP Conference Proceedings 2120, 080010. https://doi.org/10.1063/1.5115748.
Day RA, Underwood AL (1999) Qualitative Analysis. New Delhi, Prentice-Hall of India Pvt. Ltd.
Ikanone CEO, Oyekan PO (2014) Effect of boiling and frying on the total carbohydrate, vitamin C and mineral contents of Irish (Solanum tuberosum) and sweet (Ipomea batatas) potato tubers. Nigerian Food Journal 32 (2): 33 – 39. https://doi.org/10.1016/S0189-7241(15)30115-6.
Sayyad SF, Chaudhari SR, Panda BP (2015) Quantitative determination of ethanol in arishta by using UV-visible spectrophotometer. Pharmaceutical and Biological Evaluations 2 (5): 204 – 207.
Naveed M, Mubeen S, Khan S, Khalid IAN, Seleria HAR, Bano A, Mumtaz AS (2014) Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing. Brazilian Journal of Microbiology 45 (3): 985 – 993. doi: 10.1590/s1517-83822014000300031.
Mohkam M, Nezafat N, Berenjian A, Mobasher MA, Gashemi Y (2016) Identification of Bacillus probiotics isolated from soil rhizosphere using 16S rRNA, recA, rpoB gene sequencing and RAPD-PCR. Probiotics Antimicrobial Protocol 8 (1): 8 – 18. doi: 10.1007/s12602-016-9208-z.
Wakil, Monilola S, Abiola OK (2011) Diversity of pectinolytic bacteria causing soft rot disease of vegetables in Ibadan, Nigeria. Journal of Applied Biosciences 38: 2540 – 2550.
Benada M, Boumazza B, Boudalia S, Khaladi O, Guessas B (2018) Variability of aggressiveness and virulence of Erwinia carotovora subsp. carotovorum causing the soft rot on potato tubers in the western of Algeria. International Journal of Plant Biology 9 (1): 52 – 58. https://doi.org/10.4081/pb.2018.7568.
Tournas VH (2005) Spoilage of vegetable crops by bacteria and fungi and related health hazards. Critical Reviews in Microbiology 31: 33 – 44. https://doi.org/10.1080/10408410590886024.
Rokhmah DN, Supriadi H (2015) Prospek pengembangan iles-iles (Amorphophallus muelleri Blume) sebagai upaya diversifikasi pangan di Indonesia. SIRINOV 3 (1): 1 – 10.
Claassens MMJ, Verhees J, van der Plas LHW, van der Krol AR, Vreugdenhii D (2005) Ethanol breaks dormancy of potato tuber apical bud. Journal of Experimental Botany 56 (419): 2515 – 2525. doi: 10.1093/jxb/eri245.
Kõiv V, Roosaare M, Vedler E, Kivistik PA, Toppi K, Schryer DW, Remm M, Tenson T, Mäe A (2015) Microbial population dynamics in response to Pectobacterium atrosepticum infection in potato tubers. Scientific Reports 5, 11606. doi: 10.1038/srep11606.
Peng Q, Yuan Y, Gao M (2013) Bacillus pumilus, a novel ginger rhizome rot pathogen in China. Plant Disease 97 (10): 1308 – 1315. doi: 10.1094/PDIS-12-12-1178-RE.
Muslim DSN, Hasan AM, Mahdi DNZ (2016) Antibiofilm and antiadhesive properties of pectinase purified from Pseudomonas stutzeri isolated from spoilt orange. Advances in Environmental Biology 10 (11): 91 – 98.
Schmidt J, John M (1979) Starch metabolism in Pseudomonas stutzeri. I. Studies on maltotetraose-forming amylase. Biochimica et Biophysica Acta (BBA) – Enzymology. 566 (1): 88 – 99. doi: 10.1016/0005-2744(79)90252-3.
Downloads
Published
Issue
Section
License
The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Journal of Tropical Life Science is licensed under Creative Commons Attribution-NonCommercial 4.0 International License