Level of mRNA Insulin Gene and Blood Glucose STZ-Induced Diabetic Rat are Improved by Glucomannan of Amorphophallus muelleri Blume from East Java Forest Indonesia


  • Fatchiyah Fatchiyah Universitas Brawijaya http://orcid.org/0000-0001-6241-9665
  • Damai Aulia Nurmasari
  • Nuri Masruro
  • Rista Nikmatu Rohmah
  • Lidwina Faraline Triprisila
  • Mulyati Mulyati
  • Takahisa Yamada
  • Takashi Ohta




Beta pancreatic cells, glucomannan, mRNA of insulin gene, stz- induced diabetic rat


Diabetes mellitus is one of metabolic disorder with high level of blood sugar glucose and reduces pancreatic-insulin levels that are altered by unhealthy food and lifestyle. To manage the blood glucose and insulin level, we need the diabetes dietary management related with insulin gene cascade. The purpose of this study was to investigate the influence glucomannan fiber to mRNA level of the insulin gene and genes cascade expression of target cells of type 1 diabetic rat. Three-month-old male (Rattus norvegicus strain Wistar) were fed with fiber of glucomannan of Amorphophallus muelleri Blume with dosage 0,06g/kg BW and 0.12g/kg BW every day during one month. RNA dot blotting with specific cDNA probe was performed mRNA level of insulin gene. The result of this study showed that glucomannan could reduce of body weight and blood glucose level significantly. The mRNA level of insulin gene of diabetic rats-treated 0.12g/kg BW glucomannan fiber dosage was the highest significance level among of all groups. In histopathological analysis, glucomannan was increasing significantly in IRS-1 level expression proliferate properly and consistently increasing of the PI3-K expression level in treated diabetic rats. This study indicates that the 0.12g/kg BW glucomannan was optimum dosage that effectively as role for alternative high-fiber therapy of type 1 diabetic through insulin receptor tyrosine kinase pathway.


Author Biography

Fatchiyah Fatchiyah, Universitas Brawijaya



Hsu I R, Kim SP, Kabir M, Bergman RN (2007) Metabolic syndrome, hyperinsulinemia and cancer. American Journal of Clinical Nutrition 86 (3): 867 – 871. doi: 10.1093/ajcn/86.3.867S.

World Health Organization (WHO) 2017 Diabetes 15. In Diabetes (Vol. 2014, p. http://www.who.int/mediacentre/factsheets/fs312/en). Accessed date: September 2017.

Gavin JR (2001) Pathophysiologic mechanisms of post-prandial hyperglycemia. The American Journal of Cardiology 88 (6): 4 – 8. doi: 10.1016/S00029149(01)01830-6.

Pollock RF, Erny-Albrecht KM, Kalsekar A et al. (2011) Long-acting insulin analogs: a review of “real-world†effectiveness in patients with type 2 diabetes. Current Diabetes Reviews 7 (1): 61 – 74. doi: 10.2174/157339911794273892

Boucher J, Kleinridders A, Kahn, CR (2014) Insulin receptor signaling in normal and insulin resistant. Cold Spring Harbor Perspectives in Biology 6: a009191. doi: 10.1101/cshperspect.a009191

Khanna S, Tester RF (2006) Influence of purified konjac glucomannan on the gelatinisation and retrogradation properties of maize and potato starches. Food Hydrocolloids 20: 567–576. doi: 10.1016/j.foodhyd.2005.05.004

Katsuraya K, Okuyama K, Hatanaka K et al. (2003) Con-stitution of konjac glucomannan: Chemical analysis and 13C NMR spectroscopy. Carbohydrate Polymers 53 (2): 183–189. doi: 10.1016/S0144-8617(03)00039-0

Chandalia M, Garg A, Lutjohann D et al. (2000) Beneficial effects of high dietary fiber intake in patients with type 2 diabetes mellitus. New England Journal of Medicine 342 (19): 1392–1398. doi: 10.1056/NEJM200005113421903.

Yoshida M, Vanstone CA, Parsons WD et al. (2006) Effect of plant sterols and glucomannan on lipids in individuals with and without type II diabetes. European Journal of Clinical Nutrition 60: 529 – 537. doi: 10.1038/ sj.ejcn.1602347.

Fatchiyah F, Zubair M, Shima Y et al. (2006) Differential gene dosage effects of Ad4BP/SF-1 on target tissue development 341 (4): 1036 – 1045. doi: 10.1016/j.bbrc.2006.01.058

Aoe S, Kudo H, Sakurai, S (2015) Effects of liquid konjac on parameters related to obesity in diet-induced obese mice. Bioscience, Biotechnology and Biochemistry 79 (7): 1141 – 1146. doi: 10.1080/09168451.2015.1018119

Morinaga H, Ohta T, Matsui K et al. (2009) Effect of food restriction on adipose tissue in spontaneously diabetic torii fatty rats. Experimental Diabetes Research 2009: 715057. doi: 10. 1155/2009 /715057.

Ohta T, Toriniwa Y, Ryumon N et al. (2017) Maternal high-fat diet promotes onset of diabetes in rat offspring. Animal Science Journal 149–155. doi: 10.1111/asj.12606.

Miyajima K, Teoh SH, Yamashiro H et al. (2018) Effects on glycemic control in impaired wound healing in spontaneously Diabetic Torii (SDT) fatty rats. Med Arch. 72 (1): 4-8. doi: 10.5455/medarh.2018.72.4-8.

Fang W, Wu P (2004) Variations of Konjac glucomannan (KGM) from Amorphophallus konjac and its refined powder in China. Food Hydrocolloids 18 (1): 167 – 170. doi: 10.1016/S0268-005X(03)00044-4.

Shah AD, Langenberg C, Rapsomaniki E et al. (2015) Type 2 diabetes and incidence of cardiovascular diseases: A cohort study in 1·9 million people. The Lancet Diabetes & Endocrinology 3 (2): 105 – 113. doi: 10.1016/ S2213-8587(14)70219-0.

Wang CH, Lai P, Chen ME, Chen HL (2008) Antioxidative capacity produced by Bifidobacterium- and Lactobacillus acidophilus-mediated fermentations of konjac glucomannan and glucomannan oligosaccharides. Journal of the Science of Food and Agriculture 88 (7): 1294 – 1300. doi: 10.1002/jsfa.3226.

Fatchiyah F, Christian N, Soeatmadji D (2013) Reducing IRS-1 activation cause mutation of Tyrosine Kinase Domain hINSR gene on type-2 diabetes mellitus patients. Bioinformation 9 (17): 853 – 857.

Kong W, Zhang H, Song D et al. (2009) Berberine reduces insulin resistance through protein kinase C – dependent up-regulation of insulin receptor expression. Metabolism 58 (1): 109–119. doi: 10. 1016/j. metabol. 2008.08.013

Saltiel AR, Kahn CR (2001) Insulin signaling and the regulation of glucose and lipid metabolism. Nature 414 (6865): 799 – 806. doi: 10.1038/414799a

Martin S, Millar CA, Lyttle CT et al. (2000) Effects of insulin on intracellular GLUT4 vesicles in adipocytes: Evidence for a secretory mode of regulation. Journal of Cell Science 113: 3427 – 3438. doi: 10.1074/jbc.270.50.3019.