Ovitrap Index and Transovarial Transmission Rate of Dengue Virus of Male and Female Aedes aegypti Mosquitoes in Makassar, South Sulawesi, Indonesia
DOI:
https://doi.org/10.11594/jtls.09.01.13Keywords:
transovarial transmission, dengue virus, Aedes aegypti, RT-PCRAbstract
The incidence rate (IR) of dengue hemorrhagic fever (DHF) in Indonesia has increased in recent years and still causes a health problem. Makassar, as one of the big cities in Indonesia, is also experiencing of DHF cases. According to the Health Office of Makassar City, DHF cases during 2014 – 2016 has increased from 139 to 250 cases. The defense mechanism of dengue virus in transovarial transmission from female mosquitoes to eggs is estimated to be one of the factors that increase the rate of DHF. In addition, the circulation of the Aedes aegypti mosquitoes can also affect the increase of the rate. This study aims are to identify ovitrap index and to find out how much the vertical transmission of dengue virus by measuring the minimum infection rate (MIR) and the distribution of four dengue virus serotype in Ae. aegypti mosquitoes in the city of Makassar, South Sulawesi, as well as their association with the incidence rate (IR) in the area. This observational research uses ecological study with cross-sectional approach. The samples of the study are male and female mosquitoes from Ae.aegypti eggs obtained from the installation of ovitrap in 30 urban villages in Makassar. Dengue virus identification was performed using a reverse transcriptase polymerase chain reaction (RT-PCR). These results showed that the ovitrap index (IO) was in the range of 0 - 66.7%. The average value of the ovitrap index outside the house (44.44%) was greater than inside house (37.22%). The results of identification of dengue virus using RT-PCR showed negative results as indicated by the non-formation of a band on 75 pools male and 78 pools female Ae. aegypti mosquitoes after electrophoresis. The results of this study indicated that the Ae. aegypti mosquito in Makassar City showed a tendency to lay eggs outside the house due to the habit of the people who put rain water shelters outside the house which could become breeding place for Ae. aegypti mosquitoes. This study also showed that there was no transovarial transmission of DENV found in male and female Ae. aegypti mosquitoes in Makassar City during November 2017 until January 2018.References
Atique S, Abdul SS, Hsu CY, Chuang TW (2016) Mete-orological influences on dengue transmission in Pakistan. Asian Pacific Journal of Tropical Medicine 9 (10): 954–961. doi: 10.1016/j.apjtm.2016.07.033.
Bangkele EY, Safriyanti N (2016) Hubungan suhu dan kelembapan dengan kejadian demam berdarah dengue (DBD) di Kota Palu Tahun 2010 – 2014. Medika Tadu-lako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan 3 (2): 40 – 50.
Bhatt S, Gething PW, Brady OJ et al. (2013) The global distribution and burden of dengue. Nature 496 (7446): 504–507. doi: 10.1038/nature12060.
Brady OJ, Gething PW, Bhatt S et al. (2012) Refining the global Spatial Limits of Dengue Virus Transmission by Evidence-Based Consensus. PLoS Neglected Tropical Diseases 6 (8): e1760. doi: 10.1371/journal.pntd.0001760.
Budiyanto A (2010) Pengaruh perbedaan warna ovitrap terhadap jumlah telur nyamuk Aedes spp. yang terperangkap. Aspirator 2 (2): 99 – 102.
Cheong Y, Burkart K, Leitão P, Lakes T (2013) Assessing weather effects on dengue disease in Malaysia. International Journal of Environmental Research and Public Health 10 (12): 6319 – 6334. doi: 10.3390/ijerph10126319.
Dinas Kesehatan Kota Makassar (2017) Profil kesehatan Kota Makassar tahun 2016. Makassar, Pemerintah Kota Makassar.
Dwinata I, Satoto TUB, Indriani C (2015) Autocidal ovitrap aktraktan rendaman jerami. The Indonesian Journal of Public Health 11 (2): 125 – 131.
Edillo FE, Sarcos JR, Sayson SL (2015) Natural vertical transmission of dengue viruses in Aedes aegypti in selected sites in Cebu City, Philippines. Journal of Vector Ecology 40 (2): 282–291. doi: 10.1111/jvec.12166.
Espinosa M, Giamperetti S, Abril M, Seijo A (2014) Vertical transmission of dengue virus in Aedes aegypti collected in Puerto Iguazu, Misiones, Argentina. Revista do Instituto de Medicina Tropical de São Paulo 56 (2): 165–167. doi: 10.1590/S0036-46652014000200013.
Gu H, Leung R, Jing Q et al. (2016) Meteorological factors for dengue fever control and prevention in South China. International Journal of Environmental Research and Public Health 13 (9): 867. doi: 10.3390/ijerph13090867.
Ibrahim E, Hadju V, Nurdin A, Ishak H (2016) Effectiveness of abatezation and fogging intervention to the larva density of aedes aegypti dengue in endemic areas of Makassar City. International Journal of Sciences 30 (3): 255 – 264.
Lanciotti RS, Calisher CH, Gubler DJ et al. (1992) Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. Journal of Clinical Microbiology 30 (3): 545 – 551.
Martins VEP, Alencar CH, Kamimura MT et al. (2012) Occurrence of natural vertical transmission of Dengue-2 and Dengue-3 Viruses in Aedes aegypti and Aedes albopictus in Fortaleza, Ceará, Brazil. PLoS ONE 7 (7): 1 – 7. doi: 10.1371/journal.pone.0041386.
Mulyatno KC, Yamanaka A, Yotopranoto S, Konishi E (2012) Vertical transmission of dengue virus in Aedes aegypti collected in Surabaya, Indonesia, during 2008 – 2011. Japanese Journal of Infectious Diseases 65 (3): 274 – 276. doi: 10.7883/yoken.65.274
Polson KA, Curtis C, Seng CM et al. (2002) The Use of ovitraps baited with hay infusion as a surveillance tool for Aedes aegypti Mosquitoes in Cambodia. Dengue Bulletin 26: 178 – 184.
Promprou S, Jaroensutasinee M, Jaroensutasinee K (2005) Climatic factors affecting dengue haemorrhagic fever incidence in Southern Thailand. Dengue Bulletin 29: 41 – 48.
Sasmono RT, Wahid I, Trimarsanto H et al. (2015) Genomic analysis and growth characteristic of dengue viruses from Makassar, Indonesia. Infection, Genetics and Evolution 32 165–177. doi: 10.1016/j.meegid.2015.03.006.
Seran MD, Prasetyowati H (2014) Transmisi transovarial virus dengue pada telur nyamuk Aedes aegypti (L.). Aspirator 1 (2): 53–58.
Soedarto (2012) Demam berdarah. Jakarta, CV. Sagung Seto.
Sorisi AMH, Umniyati SR, Satoto TBU (2013) Transovarial transmission indeks of dengue virus on Aedes aegypti and Aedes albopictus mosquitoes in Malalayang district in Manado, North Sulawesi, Indonesia. Tropical Medical Journal 1 (2): 87 – 95.
Sucipto, Dani C, Umniyati SR (2009) Deteksi transmisi transovarial virus dengue pada nyamuk Aedes aegypti (Diptera: Culicidae) jantan dan betina serta hubungannya dengan incidence rate demam berdarah di Kota Pontianak. Doctoral Thesis. Universitas Gadjah Mada
Thenmozhi V, Hiriyan JG, Tewari SC et al. (2007) Natural vertical transmission of dengue virus in Aedes albopictus (Diptera: Culicidae) in Kerala, a Southern Indian State. Japanese Journal of Infectious Diseases 60: 245 – 249.
Tjahjasari AM (2009) Deteksi dan penentuan serotipe virus dengue tipe 4 dari nyamuk Aedes Aegypti dengan menggunakan metode Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) di Kota Medan. Master Thesis. Universitas Sumatera Utara
Wanti, Sila O, Sinaga E (2016) Transovarial transmission and dengue virus serotypes in Aedes aegypti in Kupang. Jurnal Kesehatan Masyarakat 12 (1): 131–138.
WHO (2011) Comprehensive guidelines for prevention and control of dengue hemorrhagic fever revised and expand edition, India. New Delhi, Searo Technical Publication.
WHO (2017) Dengue and severe dengue. https://www.who.int/. Accessed: September 2018.
Widiastuti D, Yunianto B, Umniyati SR, Wijayanti N (2011) Sensitivity and specitivity of immunocytochemical assay for detection of dengue virus 3 in mosquito. Health Science Indonesia 2 (2): 87 – 91.
Wijayanti SP, Anandari D, Magfirah F (2017) Vertical transmission of dengue virus on field mosquitoes in Banyumas Regency, Central Java. Indonesia. International Journal of Public Health and Clinical Sciences 4 (3): 109 – 119.
Yee LY, Heryaman H, Faridah L (2017) The relationship between frequency of fogging focus and incidence of dengue hemorrhagic fever cases in Bandung in year 2010 – 2015. International Journal of Community Medicine and Public Health 4 (2): 456. doi: 10.18203/2394-6040.ijcmph20170272.
Downloads
Published
Issue
Section
License
The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Journal of Tropical Life Science is licensed under Creative Commons Attribution-NonCommercial 4.0 International License
_.png){kind=logo}
