The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity

Syukria Ikhsan Zam, Anthoni Agustien, Syamsuardi Syamsuardi, Akmal Djamaan, Irfan Mustafa

Abstract


Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).


Keywords


Diversity, endophytic bacteria, traditional medicinal plants, anti- microbial activity, anti-phytopathogens activity

Full Text:

PDF

References


Medrano EG, Bel AA (2007) Role of Pantoea agglomerans in opportunistic bacterial seed and boll rot of cotton (Gossypium hirsutum) grown in field. Journal of Applied Microbiology 102 (1): 134 – 143. doi: 10.1111/j.1365-2672.2006.03055.x.

Roper MC (2011) Pantoea stewartii subsp. stewartii: Lessons learned from xylem-dwelling pathogen of sweet corn. Molecular Plant Pathology 12 (7): 628 – 637. doi: 10.1111/j.1364-3703.2010.00698.x.

Correa VR, Majerczak DR, Ammar E et al. (2012) The bacterium Pantoea stewartii uses two different type III secretion systems to colonize its plant host and insect vector. Applied and Environmental Microbiology 78 (17): 6327 – 6336. doi: 10.1128/AEM.00892-12.

Lamessa G, Zeller W (2007) Screening rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia. Biological Control 49 (3): 336 – 344. doi: 10.1016/j.biocontrol.2007.05.014.

Chaiharn M, Chunhaleuchanon S, Lumyong S (2009) Screening siderophore producing bacteria as potential biological control agent for fungal rice pathogens in Thailand. World Journal of Microbiology and Biotechnology 25 (11): 1919 – 1928. doi: 10.1007/s11274-009-0090-7.

Munif A, Hallman J, Sikora R (2012) Isolation of endophytic bacteria from tomato and their biocontrol activities against fungal diseases. Microbiology Indonesia 6 (4): 148 – 156. doi: 10.5454/mi.6.4.2.

Tian F, Dang YQ, Zhu H et al. (2009) Genetic diversity of siderophore-producing bacteria of tobacco rhizosphere. Brazilian Journal of Microbiology 40 (2): 276 – 284. doi: 10.1590/S1517-838220090002000013.

Duffy BK, Defago G (1999) Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas flourescens biocontrol strains. Applied and Environmental Microbiology 65 (6): 2429 – 2438.

Jacobsen BJ, Zidaek NK, Larson BJ (2004) The role of Bacillus-based biological control agents in integrated pest management systems: Plant diseases. Phytopathology 94 (11): 1272 – 1275. doi: 10.1094/PHYTO.2004.94.11.1272.

Zuber P, Nakano MM, Marahiel MA (1993) Peptide antibiotic. In: Sonenshein AL, Hoch J, Losick R, eds. Bacillus subtilis and other Gram-positive bacteria. Washington, American Society for Microbiology. pp 897 – 916.

Moyne AL, Cleveland TE, Tuzun S (2004) Molecular characterization and analysis of the operon encoding the antifungal lipopeptide bacillomycin. FEMS Microbiologi Letters 234 (1): 43 – 52. doi: 10.1111/j.1574-6968.2004.tb09511.x.

Carrim AJI, Barbosa EC, Viera JDG (2006) Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Corobinha-do-campo). Brazilian Archives of Biology and Technology 49 (3): 353 – 359.

Cho KM, Hong SY, Lee SM et al. (2007) Endophytic bacteria communities in ginseng and their antifungal activity against pathogens. Microbial Ecology 54 (2): 341 – 351. doi: 10.1007/s00248-007-9208-3.

Ma L, Cao YH, Cheng MH et al. (2013) Phylogenetic diversity of bacterial endophytes of Panax notoginseng with antagonistic characteristics towards pathogens of root-rot disease complex. Antonie van Leeuwenhoek 103 (2): 299 – 312. doi: 10.1007/s10482-021-9810-3.

Sun H, He Y, Xiao Q et al. (2013) Isolation, characterization, and antimicrobial activity of endophytic bacteria from Polygonum cuspidatum. African Journal of Microbiology Research 7 (16): 1496 – 1504. doi: 10.5897/AJMR12.899.

Souza SA, Xavier AA, Costa MR et al. (2013) Endophytic bacteria diversity in banana 'Prata Ana' (Musa spp.) roots. Genetics and Molecular Biology 36 (2): 252 – 264. doi: 10.1590/S1415-47572013000200016.

Moat AG, Foster JW, Spector MP (2002) Microbial physiology. 4th Edition. New York, John Wiley and Sons, Inc. Publication.

Magnani GS, Didonet CM, Cruz LM et al. (2010) Diversity of

endophytic bacteria in Brazilian sugarcane. Genetics and Molecular Research 9 (1): 250 – 258. doi: 10.4238/vol9-1gmr.

de Melo Pereira GV, Magalhaes KT, Lorenzetti ER et al. (2012) A multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. Microbial Ecology 63 (2): 405 – 417. doi: 10.1007/s00248-011-9919-3.

Thomas P, Soly TA (2009) Endophytic bacteria associated with growing shoot tips of banana (Musa sp.) cv. Grand Naine and the affinity of endophytes to the host. Microbial Ecology 58 (4): 952 – 964. doi: 10.1007/s00248-009-9559-z.

Xia Y, DeBolt S, Dreyer J et al. (2015) Characterization of culturable bacterial endophytes and their capacity to promote plant growth from plants grown using organic or conventional practices. Frontier in Plant Science 6: 490. doi: 10.3389/fpls.2015.00490.

Triana E (2005) Phylogenetic analyses of rhizobia isolated from Aeschynomene spp. Biodiversitas Journal of Biological Diversity 6 (4): 233 – 237.




DOI: http://dx.doi.org/10.11594/jtls.09.01.8

Copyright (c) 2019 Journal of Tropical Life Science