Sequence Analysis of Rifampicin Resistance Determining Region (RRDR) of Mycobacterium tuberculosis

Authors

  • Mitesh Shrestha Central Department of Biotechnology, Tribhuvan University Molecular Biotechnology Unit, Nepal Academy of Science and Technology http://orcid.org/0000-0002-2156-0416
  • Hemanta Kumari Chaudhary Central Department of Biotechnology, Tribhuvan University, Nepal Center for Molecular Dynamics Nepal, Nepal
  • Pradip Kumar Chaudhary Central Department of Biotechnology, Tribhuvan University, Nepal
  • Bal Hari Poudel Central Department of Biotechnology, Tribhuvan University, Nepal

DOI:

https://doi.org/10.11594/jtls.08.01.15

Keywords:

Mycobacterium tuberculosis, Rifampicin, rpoB, RRDR, Sequence

Abstract

Mycobacterium tuberculosis has become the cause for one of the most dreadful disease which the mankind has ever known i.e. Tuberculosis. The organism holds the ability to infect multiple organs at a time resulting in multiple symptomatic presentations in pathogenic condition while in non-pathogenic condition, it can lay dormant and remain asymptomatic. The research work presented here aimed at sequencing of Rifampicin Resistance Determining Region (RRDR) of the rpoB gene present in phenotypically multidrug resistant M. tuberculosis. The findings showed that the major point of mutations to be present within this region was at codon 516, 526, and 531. Early diagnosis of multidrug resistance in any pathogen has become a pre – requisite for proper treatment and efficient elimination of pathogenic organisms from the host with minimal toxicity. Similarly, understanding the mutation dynamics of target genes also help in novel drug design and discovery.

Author Biographies

Mitesh Shrestha, Central Department of Biotechnology, Tribhuvan University Molecular Biotechnology Unit, Nepal Academy of Science and Technology

Molecular Biotechnology Unit, Nepal Academy of Science and Technology, Assistant Research Fellow. 

 

Hemanta Kumari Chaudhary, Central Department of Biotechnology, Tribhuvan University, Nepal Center for Molecular Dynamics Nepal, Nepal

Center for Molecular Dynamics Nepal, Research Associate.

Bal Hari Poudel, Central Department of Biotechnology, Tribhuvan University, Nepal

Central Department of Biotechnology, Assistant Professor.

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Published

2018-01-05

Issue

Vol. 8 No. 1 (2018)

Section

Articles

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