Bioactivity of Citrus hystrix D.C. Leaf Extract Against Cigarette Beetle Lasioderma serricorne (F.)

Silvi Ikawati, Moh. Syamsu Dhuha, Toto Himawan

Abstract


The major control of pest stored Lasioderma serricorne for using synthetic pesticides, look like phosphine. Although quite effective, synthetic pesticides have a negative effect on the environment, such as pest re-sistance, deadly natural enemies, residues that are harmful to the environment and human health. The use of botanical pesticides as an alternative control be the focus this study. Botanical pesticides are selective to the target, safe for non-target insects and the environment. This research studies the repellent and fumigant activi-ty of the leaf extract of Citrus hystrix against all L. serricorne life stage. The results of GC-MS analysis of leaf crude extracts of C. hystrix with N-hexane solvent showed the highest percentage in the citronellal compound (86.43%). C. hystrix leaf extract showed stronger fumigant against pupae and eggs, compared adults, and larvae. The toxicity of the leaf extract of C. hystrix increased with increasing concentration. At a concentration of 60 ppm, fumigant activity showed the toxicity of 98.75% (pupae), 93.75% (eggs), 86.25% (adults) and 76.25% (larvae). Sequentially the LC50 value of fumigant activity from the highest to the lowest as follows; larvae 47.56 ppm, adults 43.42 ppm, eggs 31.61 and pupae 29.63 ppm. Extract of leaves of C. hystrix, have character repellent against L. serricorne. At a concentration of 60 ppm the IR value of 66% including repellent class IV, which means strong repellent level. Based on the results of the research, extracts of leaves of C. hystrix has a fumigant activity and repellent for controlling L. serricorne.


Keywords


Lasioderma serricorne, botanical insecticide, Citrus hystrix, fumigant, repellent, life stage

Full Text:

PDF

References


Dimetry NZ, Barakat AA, El-Metwaly HE et al. (2004) Assessment of damage and losses in some medicinal plants by the cigarette beetle (Lasioderma serricorne F.). Bulletin of the national Research Centre Egypt 29: 325 – 333.

Ashworth JR (1993) The biology of Lasioderma serricorne. Journal of Stored Products Research 29 (4): 291 – 303. doi: 0.1016/0022-474X(93)90044-5.

Lo PK (1986) Recognition and control of insect pests in stored grains. Post-harvest prevention of paddy/rice loss 171-186. Taipe, Taiwan Council of Agriculture - Asian Productivity Organization.

Gasch T (2014) The use of semiochemicals for stored product protection. In Conference of Siebentes Nachwuchswissenschaftlerforum: 26 – 28 November 2014; Quedlinburg, Germany.

Imai T, Harada H (2006) Low-temperature as an alterna-tive to fumigation to disinfect stored tobacco of the cigarette beetle, Lasioderma serricorne (F.) (Coleoptera: Anobiidae). J. Applied Entomology and Zoology 41: 87 – 91. doi: 10.1303/aez.2006.87.

Shaaya E, Kostjukovski M, Eilberg J, Sukprakarn C (1997) Plant oils as fumigants and contact insecticides for the control of stored-product insects. Journal of Stored Products Research 33 (1): 7 – 15. doi: 10.1016/S0022-474X(96)00032-X.

Rajendran S, Narasimhan KS (1994) Phosphine resistance in the cigarette beetle Lasioderma serricorne (Coleoptera: Anobiidae) and overcoming control failures during fumigation of stored tobacco. International Journal of Pest Management 40 (2): 207 – 210. doi: 10.1080/09670879409371883.

Ebadollahi A, Safaralizadeh MH, Pourmirza AA (2010) Fumigant toxicity of Lavandula stoechas L. oil against three insect pests attacking stored products. Journal of Plant Protection Research 50 (1): 56 – 60. doi: 10.2478/v10045-010-0010-8.

Rajendran S, Sriranjini V (2008) Plant products as fumigants for stored-product insect control. Journal of Stored Products Research 44 (2): 126 – 135. doi: 10.1016/j.jspr.2007.08.003.

Isman MB (2006) Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51: 45 – 66. doi: 10.1146/annurev.ento.51.110104.151146.

Papachristos DP, Stamopoulos DC (2002) Repellent, toxic and reproduction inhibitory effect of essential oil vapours on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Products Research 38 (2): 117 – 128. doi: 10.1016/S0022-474X(01)00007-8.

Weaver KD, Dunkel VF, Potter CR, Ntezurubanza L (1994) Contact and fumigant efficacy of powdered and in-tact Ocimum canum Sims (Lamiales: Lamiaceae) against Zabrotes subfasciatus (Boheman) adults (Coleoptera: Bruchidae). Journal of Stored Products Research 30 (3): 243 – 252. doi: 10.1016/0022-474X(94)90052-J.

Saxena RC, Dixit OP, Harshan V (1992) Insecticidal action of Lantana camara against Callosobruchus chinensis (Coleoptera: Bruchidae). Journal of Stored Products Research 28 (4): 279 – 281. doi: 10.1016/0022-474X(92)90009-F.

Huang Y, Tan JMWL, Kini RM, Ho SH (1997) Toxic and antifeedant action of nutmeg oil against Tribolium castaneum (Herbst) and Sitophilus zeamais Motsch. Journal of Stored Products Research 33 (4): 289 – 298. doi: 10.1016/S0022-474X(97)00009-X.

Ndungu M, Lwande W, Hassanali A et al. (1995) Cleome monophylla essential oil and its constituents as tick (Rhip-icephalus appendiculatus) and maize weevil (Sitophilus zeamais) repellents. Entomologia Experimentalis et Appli-cata 76 (3): 217 – 222. doi: 10.1111/j.1570-7458.1995.tb01965.x

Regnault-Roger C (1997) The potential of botanical essen-tial oils for insect pest control. Integrated Pest Management Reviews 2 (1): 25 – 34. doi: 10.1023/A:1018472227889.

Regnault-Roger C, Hamraoui A (1994) Inhibition of reproduction of Acanthoscelides obtectus Say (Coleoptera), a kidneybean (Phaseolus vulgaris) bruchid, by aromatic essential oils. Crop Protection 13 (8): 624 – 628. doi: 10.1016/0261-2194(94)90009-4.

Paranagama P, Abeysekera T, Nugaliyadde L, Abeywickrama K (2003) Effect of the essential oils of Cymbopogon citratus, Cymbopogon nardus and Cinnomomum zeylanicum on pest incidence and grain quality of rough rice (paddy) stored in an enclosed seed box. Journal: Food, Agriculture and Environment 1 (2): 134 – 136.

Kerdchoechuen O, Laohakunjit N, Singkornard S, Matta FB (2010) Essential oils from six herbal plants for biocontrol of the maize weevil. HortScience 45 (4): 592 – 598.

Abbott WS (1925) A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18 (2): 265 – 267. doi: 10.1093/jee/18.2.265a.

Pascual-Villalobods MJ, Robledo A (1998) Screening for anti-insect activity in Mediterranean plants. Industrial Crops and Products 8 (3): 183 – 194. doi: 10.1016/S0926-6690(98)00002-8.

Juliana G, Su HCF (1983) Laboratory studies on several plant materials as insect repellents for protection of cereal grains. Journal of Economic Entomology 76 (1): 165 – 175. doi: 10.1093/jee/76.1.154.

Chi H (1997) Probit analysis. Taiwan, National Chung Hsing University.

Stefanazzi N, Taedoro S, Andriana F (2011) Composition and toxic, repellent and feeding deterrent activity of essential oils against the stored-grain pests Tribolium castaneum (Coleoptera: Tenebrionidae) and Sitophilus oryzae (Coleoptera: Curculionidae). Pest Management Science 67 (6): 639 – 646. doi: 10.1002/ps.2102.

Koul O, Walia S, Dhaliwal GS (2008) Essential oils as green pesticides: potential and constraints. Biopesticides International 4 (1): 63 – 84.

Loh FS, Awang RM, Omar D, Rahmani M (2011) Insecticidal properties of Citrus hystrix DC leaves essential oil against Spodoptera litura Fabricius. Journal of Medicinal Plants Research 5: 3739 – 3744.

Sato A, Asano K, Sato T (1990) The chemical composition of Citrus hystrix DC (Swangi). Journal of Essential Oil Research 2 (4): 179 – 183. doi: 10.1080/10412905.1990.9697857.

Pratesa HT, Santosa JP, Waquila JM et al. (1998) Insecticidal activity of monoterpenes against Rhyzopertha domi-nica (F.) and Tribolium castaneum (Herbst). Journal of Stored Products Research 34 (4): 243 – 249. doi: 10.1016/S0022-474X(98)00005-8.

Dhar R, Dawar H, Garg S et al. (1996) Effect of volatiles from neem and other natural products on genotrophic cycle and oviposition of Anopheles stephensi and An. culicifacies (Diptera: Culicidae). Journal of Medical Entomology 33 (2): 195 – 201. doi: 10.1093/jmedent/33.2.195.

Michelraj S, Sharma RK (2006) Fumigant toxicity of neem formulations against Sitophilus oryzae and Rhyzopertha dominica. Journal of Agricultural Technology 2 (1): 1 – 16.

Henn T, Weinzieri R (1989) Botanical insecticides and insecticidal soaps. Illionis, University of Illinois at Urbana-Champaign, College of Agriculture, Cooperative Extension Service.

Lu JH, Su XH, Zhong JJ (2012) Fumigant acivity of Elsholtzia stauntonii extract againts Lasioderma serricorne. South African Journal of Science 108 (7/8): 103 – 106. doi: 10.4102/sajs.v108i7/8.556.

Gautam SG, Opit GP, Tebbets JS et al. (2014) Egg morphology of key stored-product insect pests of the United States. Annals of the Entomological Society of America 107 (1): 1 - 10. doi: 10.1603/AN13103.

Kucerova Z, Stejskal V (2010) External egg morphology of two stored-product anobiids, Stegobium paniceum and Lasioderma serricorne (Coleoptera: Anobiidae). Journal of Stored Products Research 46 (3): 202 – 205. doi: 10.1016/j.jspr.2010.05.002.

Cabrera BJ (2001) Cigarette Beetle, Lasioderma serricorne (F.) (Insecta: Coleoptera: Anobiidae). http://edis.ifas.ufl.edu/in384. Accessed: July 2017.

Tapondjou AL, Adler C, Fontem DA et al. (2005) Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. Journal of Stored Products Research 41 (1): 91 – 102. doi: 10.1016/j.jspr.2004.01.004.

Buatone S, Indrapichate K (2011) Protective effects of mintweed, kitchen mint and kaffir lime leaf extracts against rice weevils, Sitophilus oryzae, in stored, milled rice. International Journal of Agriculture Sciences 3 (3): 133 – 139.

Maia MF, Moore S J (2011) Plant-based insect repellents: A review of their efficacy, development and testing. Malar-ia Journal 10 (Suppl 1): S11. doi: 10.1186/1475-2875-10-S1-S11.




DOI: http://dx.doi.org/10.11594/jtls.07.03.01

Copyright (c) 2017 Journal of Tropical Life Science