Tetracera scandens as a Medicinal Plant: Secretory Structures, Histochemistry, and Antibacterial Activity

Evi Muliyah, Sulistijorini Sulistijorini, Yohana Cecilia Sulistyaningsih, Mohamad Rafi

Abstract


Tetrascera scandens, a member of Dilleniaceae, is used for traditional medicine; the stem is utilized by the Anak Dalam tribe of Jambi Province, Sumatera island, Indonesia, to treat diarrhea symptoms. The aims of this study were to identify the secretory structures, histochemical aspects, and the antibacterial potency of T. scandens stem. Histological study of the secretory structures of T. scandens stem was carried out. The species has idioblast cells and trichomes as its secretory structures. Histochemical analysis indicated the substance secreted by T. scandens idioblast cells mainly contains alkaloids, terpenoids, and phenols. Trichomes of T. scandens only contain flavonoids. The antibacterial activity of methanol extracts was tested against Staphyllococcus aureus and Escherichia coli. Different concentration of extracts was tested using the well diffusion method. According to the results, 100 mg/mL T. scandens extract showed the best inhibitory activity with a maximum inhibition zone of 17.7 mm against S. aureus and of 12.5 mm against E. coli. This study provides scientific evidence that the stem of T. scandens has antibacterial activity and justifies its use by the local community.


Keywords


Tetrascera scandens, diarrhea medicine, secretory structures, histochemistry, antibacterial activity

Full Text:

PDF

References


Nguyen MT, Nguyen NT (2013) A new lupane triterpene from Tetracera scandens L., xanthine oxidase inhibitor. Natural Product Research 27 (1): 61 – 67. doi: 10.1080/14786419.2011.652960.

Umar A, Ahmed QU, Muhammad BS et al. (2010) Anti-hyperglycemic activity of the leaves of Tetracera scandens Linn. Merr. (Dilleniaceae) in alloxan induced diabetic rats. Journal of Ethnopharmacology 131 (1): 140 – 145. doi: 10.1016/j.jep.2010.06.016.

Nguyen MT, Awale S, Tezuka Y et al. (2004) Xanthine oxidase inhibitory activity of Vietnamese medicinal plants. Biological and Pharmaceutical Bulletin 27 (9): 1414 – 1421. doi: 10.1248/bpb.27.1414.

Lee MS, Kim CH, Hoang DM et al. (2009) Genistein-derivatives from Tetracera scandens stimulate glucose-uptake in L6 myotubes. Biological and Pharmaceutical Bulletin 32 (3): 504 – 508. doi: 10.1248/bpb.32.504.

Kwon HS, Park JA, Kim JH, You JC (2011) Identification of anti-HIV and anti-Reverse Transcriptase activity from Tetracera scandens. Biochemistry and Molecular Biology Reports 45 (3): 165 – 170. doi: 10.5483/BMBRep.2012.45.3.165.

Yeshwante SB, Juvekar AR, Pimprikar RB et al. (2009) Anti-diarrheal activity of methanolic and aqueous extracts of Dillenia indica L. Research Journal of Pharmacology and

Pharmacodynamics 3: 140 – 142.

Dickison WC (2000) Integrative plant anatomy 1st ed. Cambridge, Academic Press.

Vermeer J, Peterson RL (1979) Glandular trichomes on the in florescence of Chrysanthemum morifolium cv. Dramatic (Compositae). II. Ultrastructure and histochemistry. Canadian Journal of Botany 57 (7): 714 – 729. doi: 10.1139/b79-091.

Ascensao L, Marques N, Pais MS (1995) Glandular trichomes on vegetative and reproductive organs of Leonotis leonurus (Lamiaceae). Annals of Botany 75 (6): 619 – 626. doi: 10.1006/anbo.1995.1067.

Kjaer A, Grevsen K, Jensen M (2012) Effect of external stress on density and size of glandular trichomes in full-grown Artemisia annua, the source of anti-malarial artemisinin. AoB PLANTS 2012: pls018. doi: 10.1093/aobpla/pls018.

Corsi G, Bottega S (1999) Glandular hairs of Salvia officinalis: New data on morphology, localization, and histo-chemistry in relation to function. Annals of Botany 84 (5): 657 – 664. 10.1006/anbo.1999.0961.

Willmer CM (1983) Stomata. London, Longman Group Limited.

Harborne JB (1987) Metode fitokimia: Penuntun cara modern menganalisis tumbuhan. Bandung, ITB Press.

Furr Y, Mahlberg PG (1981) Histochemical analysis of laticifers and glandular trichomes in Cannabis sativa. Journal of Natural Products 44 (2): 153 – 159. doi: 10.1021/np50014a002.

Johansen DA (1940) Plant microtechnique 1st ed. New York, McGraw-Hill.

Guerin HP, Delaveau PG, Paris RR (1971) Localizations histochimiques: Proceeds simples de localization de pigments flavoniques. Application á quelquesphanérogrames. Bulletin de la Societe Botanique de France 118 (1 – 2): 29 – 36. doi: 10.1080/00378941.1971.10838874.

Verpoorte R, Tsoi ATA, van Doorne H, Svendsen AB (1982) Medicinal plants of Surinam. I. Antimicrobial activ-ity of some medicinal plants. Journal of Ethnopharmacolo-gy 5 (2): 221 – 226. doi: 10.1016/0378-8741(82)90046-0.

Khafagi IK (2007) Generation of alkaloids-containing idioblasts during cellular morphogenesis of Peganum harmala L. cell suspension cultures. American Journal of Plant Physiology 2 (1): 17 – 26. doi: 10.3923/ajpp.2007.17.26.

Iranbaskh A, Oshagi MA, Majd M (2006) Distribution atropine and scopolamine in different organs and stages of development in Datura stramonium L. (Solanaceae). Structure and ultrastructure of biosynthesizing cells. Acta Biologica Cracoviensia Series Botanica 48 (1): 13 – 18.

Serrato-Valenti G, Bisio A, Cornara L, Ciarallo G (1997) Structural and histochemical investigation of the glandular trichomes of Salvia aurea L. leaves, and chemical analysis of the essential oil. Annals of Botany 79 (3): 329 – 336. doi: 10.1006/anbo.1996.0348.

Monteiro WR, Castro MDM, Cristina S et al. (2001) De-velopment and some histochemical aspects of foliar glandular trichomes of Stevia rebaudiana (Bert.) Bert.-Asteraceae. Brazilian Journal of Botany 24 (3): 349 – 357. doi: 10.1590/S0100-84042001000300013.

Kulip J, Fan LN, Manshoor N et al. (2010) Medicinal plants in Maliau Basin, Sabah, Malaysia. Journal of Tropical Biology and Conservation 6: 21 – 33.

Facchini PJ (2001) Alkaloids biosynthesis in plants: Biochemistry, biology cell, molecular regulation and metabol-ic engineering application. Annual Review of Plant Physi-ology and Plant Molecular Biology 52 (1): 29 – 66. doi: 10.1146/annurev.arplant.52.1.29.

Zobel AM (1985) Localizations of phenolic compounds in tannin secreting cells from Sambucus racemosa shoots. Annals of Botany 57 (6): 801 – 810. doi: 10.1093/oxfordjournals.aob.a087163.

Gersbach PV, Wyllie SG, Sarafis V (2001) A new histo-chemical method for localization of the site of monoter-pene phenol accumulation in plant secretory structures. Annals of Botany 88 (4): 521 – 525. doi: 10.1006/anbo.2001.1480.

Lima CC, Lemos RPL, Conserva LM (2014) Dilleniaceae family: An overview of its ethnomedicinal uses, biological and phytochemical profile. Journal of Pharmacognosy and Phytochemistry 3 (2): 181 – 204.

Srivastava SD (1981) Flavonoids from the stem of Dillenia pentagyna. Phytochemistry 20 (10): 2445. doi: 10.1016/S0031-9422(00)82693-X.

Aneja KR, Sharma C, Joshi R (2012) Antibacterial activity of Terminalia arjuna Wight & Arn.: An ethnomedicinal plant against pathogens causing ear infection. Brazilian Journal of Otorhinolaryngology 78 (1): 68 – 74. doi: 10.1590/S1808-86942012000100011.

Khan JA, Kumar N (2011) Evaluation of antibacterial properties of extracts of Piper betel leaf. Journal of Pharmceutical and Biomedical Science 11 (1): 1 – 3.

Decad GM, Nikaido H (1976) Outer membrane of gram-negative bacteria. XII: Molecular-sieving function of cell wall. Journal of Bacteriology 128 (1): 325 – 336.

Payne JW, Gilvarg C (1968) Size restriction on peptide utilisation in Escherichia coli. Journal of Biological Chemistry 243 (23): 6291 – 6299.

Scherrer R, Gerhardt P (1971) Molecular sieving by the Bacillus megaterium cell wall and protoplast. Journal of Bacteriology 107 (3): 718 – 735.

Fardiaz S, Suliantri, Dewanti R (1988) Senyawa Anti-mikroba. Bogor, Laboratorium Pangan, Pusat Antar Pangan dan Gizi - Institut Pertanian Bogor.

Pelczar MJ, Chan ECS (1988) Dasar-dasar mikrobiologi Jilid

Jakarta, UI Press.

Wiart C, Mogana S, Khalifah S et al. (2004) Antibacterial screening of plants used for traditional medicine in the state of Perak, Peninsular Malaysia. Fitoterapia 75 (1): 68 – 73. doi: 10.1016/j.fitote.2003.07.013.

Kushima H, Nishijima CM, Rodrigues CM et al. (2009) Davilla elliptica and Davilla nitida: Gastroprotective, anti-inflammatory, immunomodulatory and anti-Helicobacter pylori action. Journal of Ethnopharmacology 123 (3): 430 – 438. doi: 10.1016/j.jep.2009.03.031.

Ahmad F, Emrizal, Sirat HM et al. (2014) Antimicrobial and anti-inflammatory activities of Piper porphyrophyllum (Fam. Piperaceae). Arabian Journal of Chemistry 7 (6): 1031 – 1033. doi: 10.1016/j.arabjc.2010.12.032.

Djoukeng JD, Abou-Mansour E, Tabacchi R et al. (2005) Antibacterial triterpenes from Syzygium guineense (Myrtaceae). Journal of Ethnopharmacology 101 (1 – 3): 283 – 286. doi: 10.1016/j.jep.2005.05.008.

Bone K, Mills S (2013) Principles and practice of phyto-theraphy: Modern herbal medicine. New York, Elsevier.




DOI: http://dx.doi.org/10.11594/jtls.08.01.12

Copyright (c) 2018 Journal of Tropical Life Science