Pollen Viability of Aeschynanthus tricolor Hook.

Vitri Garvita Gandadikusumah, Hary Wawangningrum, Sri Rahayu

Abstract


Aeschynanthus (Gesneriaceae) is climbing or trailing epiphytes from the tropical forests of South-East Asia. Several species are widely cultivated for their attractive flowers, which usually scarlet with long corolla tubes and probably bird pollinated.This study was carried out on Aeschynanthus tricolor Hook. to determine in vitro germinability of (1) pollen collected from flowers at anthesis, on the day before anthesis, or up to five days after anthesis and (2) pollen collected on the day of anthesis and then stored at -20oC for 127 days. Germinability tests were carried out on pollen grains after stored for four hours in 10% sucrose solution. The highest in vitro germination percentage observed was pollen collected on the day of anthesis (96.3%) and the lowest was pollen collected on the fifth day after anthesis (5.6%). The germination percentage of pollen after stored at -20oC was 98% at beginning and 22% after 127 days. From this research we can conclude that pollen of A. tricolor can be successfully collected and then stored for some time, will be practical benefit for plant breeding and conservation purposes.

Keywords


Aeschynanthus tricolor, pollen germinability, storage, in vitro, sucrose

Full Text:

PDF

References


Denduangboripant J, Cronk QCB (2000) High intraindividual variation in internal transcribed spacer sequences in Aeschynanthus (Gesneriaceae): implications for phylogenetics. Proceedingsof the Royal Society Biological Sciences 267 (1451): 1407-1415. doi: 10.1098/rspb.2000.1157.

Middleton DJ (2007) A revision of Aeschynanthus (Gesneriaceae) in Thailand. Edinburgh Journal of Botany 64 (3): 363-429. doi: 10.1017/S0960428607004878.

Rahayu S, Wawangningrum H.and Garvita R.V. (2015) Morphological characteristic and flower development of Aeschynathus tricolor Hook. (Gesneriaceae). Berita Biologi 14 (3): 203-211.

Singapore Garden Society. 2015. Aeschynanthus : A Taxonomic Study. http://www.gardensingapore.org/. Accessed: December 23, 2015.

Stanley RG, Linskens HF (1974) Pollen, Biology Biochemistry Management. Berlin, Spriger-Verlag.

Ahmad S, Rana A, Sharma R, Agnihotri RK (2012) Effect of different media and boric acid on pollen germination and tube of Tribulus terrestris – a traditional medicinal plant. International Journal of Pharmaceutical Sciences Review and research 13 (2): 77-79.

Volk GM (1995) Collecting pollen for genetic resources conservation. In: Guarino L, Rao VR, Reid R, eds. (1995) Collecting plant genetic diversity: Technical guidelines.CAB International. Rome, Bioversity International. pp: 1-10.

Bellusci F, Musacchio A, Stabile R, Pellegrino G (2010) Differences in pollen viability in relation to different deceptive pollination strategies in Mediterranean orchids. Annals of Botany 106: 769-774. doi:10.1093/aob/mcq164.

Sulusoglu M, Cavusoglu A (2014) In vitro pollen viability and pollen germination in Cherry Laurel (Prunus laurocerasus L.). The Scientific World Journal 2014 (2014): 1–7. doi: 10.1155/2014/657123.

Kelly JK, Rasch A, Kalisz S (2002) Brief communication: A method to estimate pollen viability from pollen size variation. American Journal of Botany 89 (6): 1021–1023. doi: 10.3732/ajb.89.6.1021.

Baloch MJ, Lakho AR, Bhutto H, Solangi MY (2001) Impact of sucrose concentration on in vitro pollen germination of Okra, Hibiscus esculentus. Pakistan Journal of Biological Sciences 4 (4): 402–403. doi: 10.3923/pjbs.2001.402.403.

Rihova L, Hrabetova E, Tupy J (1996) Optimization of conditions for in vitro pollen germination and tube growth in potatoes. International Journal of Plant Sciences 157 (5): 561-566.

Kamrani R (2012) Study on pollen germination and pollen tube growth of five Iranian Apricot cultivars on in vitro conditions. International Conference on Applied Life Sciences (ICALS), Nejadkoorki F (Ed). pp: 299-302.

Mudi MD, Mondal S (2014) Influence of some nutrients on in vitro pollen germination of Ricinus communis L. Cibtech Journal of Bio-Protocols 3 (3): 15-20.

Kakani VG, Prasad PVV, Craufurd PQ, Wheeler TR (2002) Response of in vitro pollen germination and pollen tube growth of groundnut (Arachis hypogaea L.) genotypes to temperature. Plant, Cell and Environment 25: 1651-1661.

Vasil IK (1987) Physiology and culture of pollen. In: Giles KL, Prakash J eds. (1987) International review of cytology (Vol. 107) Pollen: Cytology and development. Florida, Academic Press.

Ali T, Ali SI (1996) Effect of sugar concentration on pollinium germination in some members of Asclepiadaceae. Pakistan Journal of Botany 28 (2): 161-165.

Bolat I, Pirlak L (1999) An investigation on pollen viability, germination and tube growth in some stone fruits. Journal of Agriculture and Forestry 23: 383–388.

Nambudiri EMV, Thomas MK (1974) Effects of chemicals on the germination of pollen grains of Torenia asiatica Linn. The Great Basin Naturalist 34 (1): 71–81.

Mondal S, Ghanta R (2012) Studies on in vitro pollen germination of Lawsonia inermis Linn. Advances in Bioresearch 3 (3): 63–66.

Soares TL, de Jesus ON, Santos-Serejo JAD, de Oliveira AJ (2013) In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.). Revista Brasileira de Fruticultura 35 (4): 1116-1126. doi: 10.1590/S0100-29452013000400023.

Mondal S, Ghanta R (2012) Studies on in vitro pollen germination of Lawsonia inermis Linn. Advances in Bioresearch 3 (3): 63–66.

Gudadhe SP, Dhoran VS (2012) Observations on pollen viability, in vitro pollen germination and pollen tube growth in Chlorophytum comosum (Thunb) Jacq. and Asparagus officinalis L. International Journal of Current Research 4 (7): 22–27.

Towil LE (1985) Low temperature and freeze-/vacuum-drying preservation of pollen. In Cryopreservation of plant cells and organs (KK Kartha Eds). United States, CRC Press.

Borghezan M, Clauman AD, Steinmacher DA, Gueraa MP, Orth AI (2011) In vitro viability and preservation of pollen grain of Kiwi (Actinidia chinensis var. Deliciosa (A. Chev.) A. Chev). Crop Breeding and Applied Biotechnology 11: 338-344. doi: 10.1590/S1984-70332011000400007.

Patel RG, Mankad AU (2014) In vitro pollen germination-a review. International Journal of Science and Research 3 (5): 304-307.

Stone LM, Seaton KA, Kuo J, McComb JA (2004) Fast pollen tube growth in Conospermum species. Annals of Botany 93: 369-378. doi:10.1093/aob/mch050.




DOI: http://dx.doi.org/10.11594/jtls.07.01.09

Copyright (c) 2017 Journal of Tropical Life Science