The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3

Liswara Neneng, Yohanes Edy Gunawan

Abstract


Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia.  These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates.  This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory.  Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control.  All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3.  Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.


Keywords


Coenzymes, Mercury Bioremediation, Pseudomonas aeruginosa KHY2, Klebsiella pneumonia KHY3

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References


Nascimento AMA, Chartone-Souza EC (2003) Operon mer: Bacterial resistance to mercury and potential for bio-remediation of contaminated environments. Genetics and Molecular Research 2 (1): 92 – 101.

Nies DH (1999) Microbial heavy-metal resistance. Applied Microbiology and Biotechnology 51 (6): 730 – 750.

Beard RR (1987) Treating gold ores by amalgamation. Arizona, Department of Mines and Mineral Resources.

Suhendrayatna (2001) Bioremoval logam berat dengan menggunakan mikroorganisme: Suatu kajian kepustakaan. In Proceedings on-Air Bioteknologi untuk Indonesia Abad 21, Collaboration between Sinergy Forum and PPI Tokyo Istitute of Technology: 1 – 14 Feb-ruary 2001.

Rajendran P, Muthukrishnan J, Gunasekaran P (2003) Microbes in heavy metal remediation. Indian Journal of Experimental Biology 41 (9): 935 – 944.

Wagner- Döbler I, von Canstein H, Li Y et al. (2000) Removal of mercury from chemical wastewater by micro-organisms in technical scale. Environmental Science and Technology 34 (21): 4628 – 4634. doi: 10.1021/es0000652.

Essa AMM, Macaskie LE, Brown NL (2002) Mechanisms of mercury bioremediation. Biochemical Society Transactions 30 (4): 672 – 674. doi: 10.1042/bst0300672

Barkay T, Susan MM, Summers AO (2003) Bacterial mercury resistance from atoms to ecosystem. Journal FEMS Microbiology Reviews 27 (2 – 3): 355 – 384.

Neneng L (2008) Exploration of potential isolates bacterial for mercury (Hg2+) bioremediation from gold mining area in Kahayan River, Central Kalimantan. Agritek Journal 16: 189 – 194.

Karigar CS, Rao SS (2011) Role of microbial enzymes in the bioremediation of pollutants: A review. Enzyme Research 2011. doi: 10.4061/2011/805187.

Berg JM, Tymoczko JL, Stryer L (2002) Biochemistry, 5th ed. New York, W.H Freeman.

Burns RG, Dick RP (2002) Enzymes in the environment: Activity, ecology, and application. Boca Raton, CRC Press.

De J, Sarkar A, Ramaiah N (2006) Bioremediation of toxic substances by mercury resistant marine bacteria. Ecotoxicology 15 (4): 385 – 389. doi: 10.1007/s10646-006-0066-4.

De J, Leonhauser J, Vardanyan L (2014) Removal of mercury in fixed-bed continuous upflow reactors by mercury-resistant bacteria and effect of sodium chloride on their performance. QScience Connect 2014: 1 – 9. doi: 10.5339/connect.2014.17.

Jan AT, Murtaza I, Ali A, Haq Q (2009) Mercury pollution: An emerging problem and potential bacterial remediation strategies. World Journal of Microbiology and Biotechnology 25 (9): 1529 – 1537. doi: 10.1007/s11274-009-0050-2.




DOI: http://dx.doi.org/10.11594/jtls.08.01.04

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