Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest

Ni Putu Ratna Ayu Krishanti, Deni Zulfina, Bramantyo Wikantyoso, Arpiwi Zulfitri, Sulaeman Yusuf

Abstract


Actinomycetes are Gram positive bacteria that have been detected in diverse ecological niches. Their member species are known to be a main source of various bioactive compounds. The discovery of Actinomycetes from diverse and unexplored resources has also been linked to increased opportunities to obtain novel bioactive compounds. Insect nest material is being investigated as a new source of novel antimicrobial producing Actinomycetes, which could be harnessed for therapeutic potential. A total of 10 Actinomycetes isolates were collected from the nest of Nasutitermes sp. in Pananjung Pangandaran Nature Reserve. These isolates were evaluated for antimicrobial activity against the challenge bacteria (Eschericia coli, Staphylococcus aureus, Bacillus subtilis, Serratia Marcescens) and fungi (Fomitopsis palustris, Fusarium oxysporum, Trichoderma viridae) by dual culture method. The result revealed that several isolate were active against fungi and bacteria. Isolate Pn-TN2 showed the highest level of antibacterial inhibition and the highest antifungal inhibition with Inhibition Rate value more than 80%. By morphological and 16S rRNA gene sequence analysis strongly suggested that the isolate Pn-TN2 belonged to Streptomyces prasinopilosus. We suggested that termite nest is a potential source of bioactive strains of cultivable Actinomycetes for future biotechnological needs.

Keywords


Actinomycetes, antimicrobial, Nasutitermes sp., Streptomyces prasinopilosus, termite nest

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References


Gupte MD, Kulkarni PR (2002). A study of antifungal antibiotic production by Streptomyces chattanoogensis MTCC 3423 using full factorial design. Lett. Appl. Microbiol. 35: 22–26.

Newman DJ, Cragg MG (2007) Natural products as sources of new drugs over the last 25 years. J. Nat. Prod. 70: 461–477.

Kumar PS, Duraipandiyan V, Ignacimuthu S (2014) Isolation, screening, and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA 7 Kaohsiung. Journal of Medical Science 30: 435–446.

Watve MG, Tickoo R, Jog MM, Bhole BD (2001). How many antibiotics are produced by the genus Streptomyces? Arch Microbiol. 176: 386-390.

Subramani R, Aalbersberg W (2012) Marine Actinomycetes: an ongoing source of novel bioactive metabolites. Microbiol. Res. 167: 571–580.

Dettner K (2011) Potential pharmaceuticals from insects and their cooccurring microorganisms. In: Vilcinskas A (ed) Insect Biotechnology. New York, Springer Press. pp 95–119.

Berlanga M, Paster BJ, Guerrero R (2009) The taxophysiological paradox: changes in the intestinal microbiota of the xylophagous cockroach Cryptocercus punctulatus depending on the physiological state of the host. Int. Microbiol. 12: 227–236.

Robert OE, Frank UO, Agbonsalo OU (2007) Influence of activities of termites on some physical and chemical properties of soils under different land use patterns: a review. Int. J. Soil Sci. 2: 1–14.

Verma M, Sharma S, Prasad R (2009) Biological alternatives for termite control: a review. Int. Biodet. Biodeg. 63: 959–972.

Khucharoenphaisan K, Sripairoj N, Sinma K (2012) Isolation and identification of Actinomycetes from termite’s gut against human pathogen. Asian J. Anim. Vet. Adv. 7 (21): 68–73.

Ramin M, Alimon AR, Sijam K, Abdullah N (2008) Filter paper degradation by bacteria isolated from local termite gut. Res. J. Microbiol. 3: 565–568.

Brauman A (2000) Effect of gut transit and mound deposit on soil organic matter transformations in the soil feeding termite: a review. Eur. J. Soil Biol. 36: 117–125.

Sujada N, Sungthong R, Lumyong S (2014) Termite nests as an abundant source of cultivable Actinobacteria for biotechnological purposes. Microbes Environ. 29 (2): 211-219.

Holt JA, Lepage M (2000) Termites and soil properties. In: Abe T, Bignell DE, Higashi M (eds) Termites: evolution, sociality, symbioses, ecology, vol 2. Dordrecht, Kluwer Academic.

Garnier‑Sillam E, Toutain F, Renoux J (1988) Comparaison de I’influence de deux termitie`res (humivore et champignonniste) surla stabilite´ struc‑ turale des sols forestiers tropicaux. Pedobiology 32: 89–97.

Harry M, Jusseaume N, Gambier B, Garnier‑Sillam E (2001) Use of RAPD mark‑ ers for the study of microbial community similarity from termite mounds and tropical soils. Soil Biol. Biochem. 33: 417–427.

Pasti MB, Pometto AL, Nuti MP, Crowford DL (1990) Lignin-solubilizing ability of Actinomycetes isolated from termite (Termitidae) gut. Appl. Environ. Microbiol. 56: 2213–2218.

Matsui T, Tanaka J, Namihira T, Shinzato N (2012) Antibiotics production by an Actinomycetes isolated from the termite gut. J. Basic Microbiol. 52: 1–5.

Schafer A, Konrad R, Kuhnigk T, Kampfer P, Hertel H, Konig H (1996) Hemicellulose-degrading bacteria and yeasts from the termite gut. J. Appl. Microbiol. 80 (33): 471–478.

Sinma K, Ishida Y, Tamura T, Kitpreechavanich V, Tokuyama, S (2011) Saccharopolyspora pathumthaniensis sp. nov., a novel Actinomycetes isolated from termite guts (Speculitermes sp.). J. Gen. Appl. Microbiol. 57: 93–100.

Hyun Kim K, Ramadhar TR, Beemelmanns C, Poulsen M, Currie CR, Clardy J (2014) Natalamycin A, an ansamycin from a termite-associated Streptomyces sp.. Chem. Sci. 5(11): 4333-4338.

Padilla MA, Rodrigues RAF, Bastos JCS, Martini MC, Barnabe ACS, Kohn LK, Uetanabaro APT, Bomfilm GF, Afonso RS, Garboggini FF, Arns CW (2015) Actinobacteria from termite mounds show antiviral activity against bovine viral diarrhea virus, a surrogate model for hepatitis C virus. Evidence-Based Complementary and Alternative Medicines 2015, article ID 745754: 1-9.

Ahmad M (1958) Key to the Indomalayan Termites. Department of Zoology University of the Punjab. Lahore.

Syaukani (2010) A Guide to the Nasus Termites (Nasutitermitinae, Termitidae) of Kerinci Seblat National Park Sumatra. Nagao Natural Environmental Foundation, Tokyo.

Kang MJ, Strap JL, Crawford DL (2010) Isolation and characterization of potent antifungal strains of the Streptomyces violaceusniger clade active against Candida albicans. J. Ind. Microbiol Biotechnol. 37: 35-41.

Hayakawa M, Yoshida Y, Iimura Y (2004) Selective isolation of bioactive soil Actinomycetes belonging to the Streptomyces violaceusniger phenotypic cluster. J. Appl. Microbiol. 96: 973–981.

Hayakawa M, and Nomura S (1987) Humic acid-vitamin agar. A new medium for the selective isolation of soil Actinomycetes. J. Ferment. Technol. 65: 501–509.

Quintana ET, Gill-Rivera DA, Vlderique AA, Villegas OL, Maldonado LA (2015) Evaluation of the antifungal and antiyeast activities from recently isolated Streptomyces. J. Pharm. Biomed. Sci. 5(11) : 867-876.

Islam MR, Jeong YT, Ryu YJ, Song CH, Lee YS (2009) Isolation, identification and optimal culture conditions of Streptomyces albidoflavus C247 producing antifungal agents against Rhizoctonia solani. Mycobiology 37(2): 114-120.

Kellner RLL (2002) Molecular identification of an endosymbiotic bacterium associated with pederin biosynthesis in Paederus sabaeus (Coleoptera: Staphylinidae). J. Insect Biochem. Molec. 32: 389–395.

Sunaryanto R, Mahsunah AH (2013) Isolation, purification, and characterization of antimicrobial substances from endophytic Actinomycetes. Makara J. Sci. 17(3) : 87-92.

Kroiss J, Kaltenpoth M, Schneider B, Schwinger M, Hertweck C, Maddula RK, Strohm E, Svatos A (2010) Symbiotic Streptomycetes provide antibiotic combination prophylaxis for wasp offspring. Nat. Chem. Biol. 6: 261-263.

Prapagdee B, Kuekulvong C, Mongkolsuk S (2008) Antifungal potential of extracellular metabolites produced by Streptomyces hygroscopicus against phytopathogenic fungi. International Journal of Biological Sciences 4(5) : 330-337.

Vicente MF, Basilio A, Cabello A, Pelaez F (2002) Microbial natural products as a source of antifungals. Clin. Microbiol. Infect. 9: 15-32.

Ettlinger L, Corbaz R, Hutter R (1958) Zur systematik der Actinomyceten 4. Eine Artenienteilung der Gattung Streptomyces Waksman and Henrici. Arch. Mikrobiol. 31: 326-358.

Hopwood DA (1999) Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico. Microbiol. 145: 2183-2202.

Nakayama M, Takahashi Y, Itoh H, Kamiya K, Shiratsuchi M, Otani G (1989) Novel antifungal antibiotics maniwamycins A and B. The Journals of Antibiotics XLII (11): 1535-1989.

Fukumoto A, Murakami C, Anzai Y, Kato F (2016) Maniwamycins: new quorum-sensing inhibitors against Chromobacterium violaceum CV026 were isolated from Streptomyces sp. TOHO-M025. The Journal of Antibiotics 69: 395-399.

Lee KE, Wood TG (1971) Termites and Soil. London, Academic Press. Pp 27, 41, 147-148.

Souza JLP, Moura CAR (2008) Predation of ants and termites by army ants, Nomamyrmex esenbeckii (Formicidae, Ecitoninae) in the Brazilian Amazon. Sociobiology 52(2): 399-402.

Noirot C, Darlington JPEC (2000) Termite nests: architecture, regulation, and defence. In: Abe T, Bignell DE, Higashi M. (eds) Termites: Evolution, Sociality, Symbioses, Ecology. Dordrecht, Kluwer Academic Publishers.




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